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Jurnal Teknologi Laboratorium
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52
Articles
Pengembangan Prekultur Oxgall sebagai Sampel Klinis untuk Deteksi Salmonella typhi dengan Metode Real-time PCR

Gunawan, Annisa Pratiwi, Djuminar, Ai, Ernawati, Ernawati, Chaidir, Lidya

Jurnal Teknologi Laboratorium Vol 7 No 2 (2018): Tahun 2018 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Demam tifoid merupakan beban kesehatan masyarakat yang signifikan di negara berpenghasilan rendah yang disebabkan oleh Salmonella enterica serotype typhi (S.typhi). Manifestasi klinis demam tifoid bervariasi dan tidak spesifik, sehingga membuat diagnosis menjadi sulit. Dengan menggunakan oxgall untuk pra-inkubasi sebagai media kultur selektif sebelum amplifikasi Real-time PCR (RT-PCR) pada wholeblood menghasilkan diagnostic yang cepat dan sensitif. Tujuan dari penelitian ini adalah untuk mengetahui kinerja oxgall sebelum amplifikasi  RT-PCR untuk deteksi Salmonella sp . Sebelum proses pengambilan sampel, dilakukan optimasi spike sampel untuk mengetahui bahwa reagen yang  digunakan baik untuk spesimen klinis. Dalam proses sampel, sampel wholeblood diambil dari  30 pasien dengan positif Widal tes. Sampel darah vena dari pasien demam tifoid diambil pada hari diagnosis; 5 ml untuk kultur darah, dan 5 ml untuk RT-PCR. Bakteri ditanam di oxgall 10% (standar mikrobiologi laboratorium klinik) dan diinkubasi selama 6 jam (37 ° C) sebelum DNA bakteri diisolasi untuk deteksi RT-PCR. Hasil penelitian menunjukkan bahwa reagen RT-PCR baik digunakan untuk sampel klinis dan kultur darah lebih baik daripada RT-PCR dengan menggunakan oxgall (hasil kultur darah positif lebih dari 24 jam). Hal ini menunjukkan bahwa harus ada penelitian lebih lanjut mengenai durasi inkubasi dan konsentrasi oxgall pada RT-PCR dan pemilihan sampel klinis.

Aktivitas Antibakteri Kulit Buah Karika Dieng terhadap Shigella flexneri dan Escherichia coli

Novalina, Dhiah, Sugiyarto, Sugiyarto, Susilowati, Ari

Jurnal Teknologi Laboratorium Vol 7 No 2 (2018): Tahun 2018 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Karika merupakan tanaman endemik Dataran Tinggi Dieng. Daun Karika telah diteliti memiliki aktivitas antibakteri terhadap bakteri penyebab diare, sedangkan kulit buah dibuang atau dijadikan campuran pakan ternak. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri kulit buah Karika terhadap bakteri penyebab keracunan, Shigella flexneri dan Escherichia coli. Sampel difraksinasi untuk memperoleh fraksi n-heksan dan etil asetat. Fraksi diuji aktivitas antibakteri nya terhadap bakteri Shigella flexneri dan Escherichia coli dengan metode sumuran. Hasil penelitian menunjukkan bahwa fraksi etil asetat dengan konsentrasi 50% memiliki daya hambat tertinggi terhadap Shigella flexneri dan Escherichia coli dibandingkan dengan konsentrasi lainnya. Berdasarkan hasil penelitian disimpulkan bahwa kulit buah Karika memiliki aktivitas antibakteri terhadap bakteri Shigella flexneri dan Escherichia coli.

Potensi Ekstrak Daun Carica Pubescens Sebagai Alternatif Antidiare Bakteri Vibrio cholerae dan Shigella dysentriae

Astuti, Tri Dyah, Hadi, Wahid Syamsul

Jurnal Teknologi Laboratorium Vol 7 No 2 (2018): Tahun 2018 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Diare akut merupakan salah satu penyebab utama morbiditas dan mortalitas. Masyarakat mulai memilih obat tradisional atau obat herbal sebagai pengobatan alternatif. Obat tradisional atau obat herbal dinilai lebih aman dan tidak memberikan efek samping seperti obat kimia. Tujuan dari penelitian ini adalah untuk mengetahui efek anti diare ekstrak daun Carica pubescens terhadap bakteri Vibrio cholerae dan Shigella dysentriae. Penelitian ini dilakukan dengan menguji aktivitas bakteri Vibrio cholerae dan Shigella dysentriae terhadap ekstrak daun Carica pubescens dengan metode sumuran yang hasilnya dapat dilihat dari terbentuknya zona hambat. Data yang diperoleh diolah menggunakan statistik uji Two Way ANOVA. Hasil penelitian menunjukkan ekstrak daun Caricap pubescens konsentrasi 100% mempunyai efek terapi terbaik karena mempunyai daya hambat terbesar terhadap bakteri Vibrio cholerae maupun Shigella dysentriae.

The Presence of Methanol In Alcoholic Beverages Analyzed Using Qualitative Method

Navianti, Diah, Tarmizi, Muhammad Ihsan, Holifah, Sinta Nur

Jurnal Teknologi Laboratorium Vol 7 No 2 (2018): Tahun 2018 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

An alcoholic beverage contains ethyl alcohol or ethanol (C2H5OH)that is produced from fermentation or distillation of sugars. In many cases, either producers or consumers add ethanol with denaturated alcohol containing methanol (CH3OH) as an additive. Methanol can cause blindness and induce comas, and it is deadly in high doses. This study aimed at investigating the presence of methanol or methyl alcohol in alcoholic beverages sold in Palembang, Indonesia. Seventeen samples collected from small shops and supermarkets were taken by accidental sampling. A chromotropic acid method was used to examine the presence of methanol. The results showed that there were 18% of the samples was positive, and 82% was negative. Based on alcohol content, the research showed that all (100%) samples of group A were negative; 33% of group B was positive, and 33% of group C was positive. The study indicated that methanol was still present in alcoholic drinks sold in markets. The government should inform the society that denatured alcohol contains methanol and, therefore, should not be feasible to consume.

Antibiotic Resistant and Plasmid Conjugative Study of Salmonella typhi

Haji Saeed Akreyi, Waleed, Younis Yousif, Samira, Assafi, Mahde

Jurnal Teknologi Laboratorium Vol 7 No 2 (2018): Tahun 2018 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

The emergence of multi-drug resistant (MDR) bacteria has endangered the efficacy of antibiotics treatment of pathogenic bacteria worldwide. The aim of this research was to investigate the incidence of Salmonella enterica serovar Typhi in Duhok city, Iraq. Specimens of blood and stool were recruited from 267 patients. S. Typhi isolates were diagnosed depending on morphology, biochemical and serological tests. S. Typhi isolates were tested for their antibiotic resistance. Multi-drug resistant S. Typhi isolates were conjugated with E. coli HB101. The plasmid profile of transconjugants was investigated. 15/267 (5.6%) S. Typhi isolates were identified. Based on their biochemical tests, S. Typhi isolates were categorized into two biotypes (I, 26.66% and II, 73.33%). Four resistance patterns were observed. The resistant pattern to ampicillin and tetracycline was the higher (46.6%). Conjugation experiment showed that all antibiotic markers were transferred from S. Typhi to E. coli HB101 with a conjugation frequency of (0.38×10-5). 13.3% of the S. Typhi isolates were multi-drug-resistant resistant and had two small plasmids. Transconjugants E. coli acquired the resistance from the multi-drug resistant S. Typhi. Antibiotics treatment of the pathogens could be hindered by the constant rise of multi-drug-resistant. Further studies are needed to study the mobile genetic elements and their contribution to antibiotics resistance.

Potensi Antibakteri Isolat Actinomycetes terhadap Aktivitas Proteolitik dan Amilolitik Escherichia Coli ATTC 25922

Bahar, Meiskha, Zulfa, Fajriati

Jurnal Teknologi Laboratorium Vol 7 No 1 (2018): Tahun 2018 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Occurs E. coli resistance to class 3 cephalosporin class antibiotics and fluoroquinolone groups. The antibiotic resistance that occurs has narrowed the choice of therapy. This study aims to determine the effect of Actinomycetes isolates on proteolytic and amylolytic enzymes E. coli ATCC 25922. This research is experimental research, qualitative tests of protease and amylase enzymes from E. coli ATCC 25922 shown by clear zones around the growing colonies. The result of ANOVA One-Way test showed a significant difference in the width of clear zone, colony zone and PER and AER score with p-value < 0,05. This indicates that Actinomycetes isolates contain compounds that can act as inhibitors of protease and amylase enzymes from E.coli ATCC 25922. It is hoped that there will be research about the identification of Actinomycetes species isolates in Bogor Botanical Garden so that later can be cultivated and produced as an antibiotics alternative.

Isolasi Candida albicans Dari Swab Mukosa Mulut Penderita Diabetes Melitus Tipe 2

Kadek Sri Jayanti, Ni, Jirna, I Nyoman

Jurnal Teknologi Laboratorium Vol 7 No 1 (2018): Tahun 2018 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Candida pathogen when immunity had decreased and changes in physiological functions, such as in diabetes mellitus. This study aims to isolation Candida albicans that collected from oral cavity of diabetic type 2 patients. This descriptive study was determined by observing the presence of Candida albicans in 30 samples of diabetic patients, which grows on Potato Dextrose Agar, then the microscopic observation by LPCB staining of yeasts, blastospores, pseudohyphae, chlamydospores and germ tubes in human serum suspension that incubated at 370C for 2-3 hours, from 30 DM patients studied, it was found positive result of Candida albicans fungus on mucosa mouth swab patient type 2 diabetes, that is 14 people with percentage 46,7%.

Imunomodulator Ekstrak Etanol Daun Mimba (Azadirachta indica) terhadap Jumlah Sel Makrofag Peritoneal pada Mencit yang Diinduksi Vaksin BCG

Abror, Yogi Khoirul, Woelansari, Evy Diah, Suhariyadi, Suhariyadi

Jurnal Teknologi Laboratorium Vol 7 No 1 (2018): Tahun 2018 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

This research was conducted to determine the immunomodulatory effect of ethanol extract of neem leaves (Azadirachta indica) to the number of peritoneal macrophages in mice wich induced by BCG vaccine. Bacillus Calmette-Guerin (BCG) vaccine contained an attenuated Mycobacterium bovis. Mycobacterium bovis belongs to the Mycobacterium Tuberculosis Complex (MTC) group that has a similar phenotype characteristic with Mycobacterium tuberculosis and similar clinical manifestations of tuberculosis.The type of the research that used in this study is laboratory experimental research with Post Test Design Design Only Control Group Design. The research was conducted at the Faculty of Veterinary Medicine of Airlangga University in July 2017 using 25 male mice divided into five groups. The dosage of ethanol extract of the neem leaves given was 200 mg / Kg BW with variation for two days, four days, and six days are given.In the result of statistical data analysis using Kruskal-walis test, it is known that the significance value p = 0,03 (p <0,05), that means immunomodulatory of ethanol extract of neem leaves (Azadirachta indica) give an effect to peritoneal macrophage cell number in mice wich induced by BCG vaccine, so that neem leaves ethanol extract can be applied to tuberculosis patients.

Optimasi Waktu Produksi Metabolit Sekunder dan Skrining Aktivitas Antibakteri Isolat Actinomycetes Rizosfer Tanaman Tin (Ficus carica)

Warsi, Warsi, Sulistyani, Nanik

Jurnal Teknologi Laboratorium Vol 7 No 1 (2018): Tahun 2018 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Some Actinomycetes isolates of a tin plant (Ficus carica L.) have been obtained, namely T24M, T18, T19, T24, T25, T34, T37, T41, and T43. The aim of this study was to optimize the production of secondary metabolites (antibiotics) and screening antibacterial activity against Methicillin-Resistant Staphylococcus aureus (MRSA) from the Actinomycetes isolate of the tin rhizosphere. The study was performed with test an activity of the culture fluid from Actinomycetes isolate against MRSA by the well method. The result of optimization secondary metabolite production showed that the second day was the best incubation time to harvest antibiotics. The results showed that bacterial isolates of T24M produced antibiotics that could inhibit MRSA growth.

Variasi Konsentrasi Alfa Siklodekstrin dan Waktu Sentrifugasi Dalam Preparasi Serum Lipemik Pada Pemeriksaan Glukosa Metode GOD-PAP

Izzati, Arfa, Riyani, Ani

Jurnal Teknologi Laboratorium Vol 7 No 1 (2018): Tahun 2018 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Examination of glucose levels of the GOD-PAP method in serum may be disrupted by the presence of turbidity caused by lipemic serum, thus causing high false serum glucose levels to result. The addition of alpha-cyclodextrin (α-CD) may bind the lipemic in serum. This study aims to find out how the concentration and time of optimal centrifugation with the addition of alpha-cyclodextrin. Added variation alpha-cyclodextrin concentration 0.5%, 1%, and 1.5% in pooled serum with variation concentration of triglycerides ± 1000 mg/dL, ± 1500 mg/dL and ± 2000 mg/dL incubated for 30 minute at 4°C. Centrifuged at 3000 rpm with variation of centrifugation time for 5 minutes, 10 minutes and 15 minutes at 4°C to form precipitate and supernatant. Then the supernatant measured the glucose GOD-PAP and triglycerides GPO-PAP using a photometer. The result were no significant differences in variations centrifugation time and variations alpha-cyclodextrin concentrations after comparison with serum glucose pooled levels (base line) in the ANOVA test, Kruskall Wallis Test and Mann Whitney test. There was no significant difference with pooled serum (base line) at the time of triglyceride concentration ± 1000 mg/dL with alpha-cyclodextrin concentration 0.5% and centrifugation time at 10 minute, triglyceride concentration ± 1500 mg/dL and ± 2000 mg/dL with alpha-cyclodextrin concentration 1%, centrifugation time at 5 minute.