Indonesian Journal of Cancer Chemoprevention
Articles by issue : Vol 2, No 1 (2011)
10
Articles
Taraxacum officinale Leaves Ethanolic Extract as Immunostimulatory Agent For Reducing Side Effect of Doxorubicin in Sprague Dawley Rats

Kasianningsih, Sri ( Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta ) , Rivanti, Erlina ( Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta ) , Pratama, Ratih Hardika ( Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta ) , Pratama, Nanda Resa ( Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta ) , Ikawati, Muthi ( Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta ) , Meiyanto, Edy ( Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta )

Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Doxorubicin as chemotherapeutic agent causes immunosuppresive. The aim  for this study to determine the effect of ethanolic extract of Taraxacum oficinale (ETO) in immunity system of Sprague Dawley  rat that induced  by doxorubicin to observe the profile of immunity cells. Sprague Dawley rats were divided into five groups each groups contain five rats : control doxorubicin group, doxorubicin dose 4,67 mg/kgBW+ ETO dose 1000 mg/kgBW, doxorubicin dose 4,67 mg/kgBW+ ETO dose 500 mg/kgBW, control extract group, and without treatment. Then the number of leukocytes, lymphocytes and neutrophils were analyzed  by hematology analyzer, whereas CD8+ T lymphocytes by flowcytometry. Results showed groups of  doxorubicin combined with ETO dose 1000 mg/kgBW and 500 mg/kgBW increased the number of leukocytes, lymphocytes, neutrophils,  cytotoxic CD8 + T cells  T cells compared  to control doxorubicin group. These data presents that etanolic extract of Jombang leaves has  immunostimulatory activity and potential as co-chemotherapy agents. Molecullar mechanism underlaying it’s immune activity need to be explored in detail.

Free Radical Scavenging Activity of Ethanolic Leaves Extract and Its Different Solvent Fractions of Piper betle L. In Vitro

Risdian, Chandra ( Research Center for Chemistry, Indonesian Institute of Sciences, Jl. Cisitu-Sangkuriang, Bandung 40135, Indonesia ) , Widowati, Wahyu ( Faculty of Medicine, Maranatha Christian University, Jl. Prof drg. Suria Sumantri No.65, Bandung 40164, Indonesia ) , Mozef, Tjandrawati ( Research Center for Chemistry, Indonesian Institute of Sciences, Jl. Cisitu-Sangkuriang, Bandung 40135, Indonesia ) , Wargaseti, Teresa Liliana ( Faculty of Medicine, Maranatha Christian University, Jl. Prof drg. Suria Sumantri No.65, Bandung 40164, Indonesia ) , Khiong, Khie ( Faculty of Medicine, Maranatha Christian University, Jl. Prof drg. Suria Sumantri No.65, Bandung 40164, Indonesia )

Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Various diseases including cancer and cardiovascular diseasesare induced by free radicals via lipid peroxidation, protein peroxidation, DNA damage, and cellular degeneration in thecells.Piper betle L.,commonly called betel,belongs to thePiperaceae family.It originated from South and South Eas tAsia,and has been used as a traditional medicine by people theredue to its medicinal properties.In the present study, free radicalscavenging activity of ethanolic leaves extract of Piper betle L.together with its different solvent fractions(hexane,ethylacetate,butanol,and water)were evaluated by DPPH freeradical scavenging assay.The ethanolic extract and the ethylacetate fraction had shown very significant DPPH (1,1)diphenyl)2)picryl)hydrazyl) radical scavenging activitycompared to the other fractions.In DPPH free radical scavengingassay,the IC50 value of the ethanolic extract and the ethyl acetate fraction were found to be 17.431g/mL and 11.53 1g/mL, respectively. The free radical scavenging activity was assumed due to the total phenolic content.The total phenoliccontent was found to be highest in the ethanol extract(277.68µgEGCG/mg)and the ethyl acetate fraction(559.38µgEGCG/mg).The results concluded that the ethanolic leaves extract of Piper  betle L. and its ethyl acetate fraction have a consider ably freeradical scavenging activity.

Antioxidant-Free Radical Scavenging of Some Euphorbiaceae Herbs

Sunarni, Titik ( Faculty of Pharmacy, Setia Budi University ) , Leviana, Fransiska ( Faculty of Pharmacy, Setia Budi University )

Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Abstract

In order to screen natural antioxidant, the research about antioxidant of some Euphobiaceae herbs,have been conducted.The air-dried herbs of Euphorbia heterophyllaL,Phyllanthus  acidus  (L.) Skeels, and Phyllanthus  buxifolius  Muell Arg were extracted with metanol.The obtained extract was concentratedand then suspended to producen-hexane, ethyl acetat and aqueous fractions. Free radical scavenger activity against DPPH (1,1diphenyl2pycrylhydrazyl) measured by spectrophotometric method and the IC50 value was determined.The compounds of active fraction had been identified by TLC method. All of theherbs showed activity as DPPH scavenger.Among these herbs,Euphorbia heterophylla L. and Phyllanthus buxifolius Muell, Arg. exhibited a strong free radical scavenging of ethyl acetatfraction with IC50 value 5,883g/ml and 4,643g/ml.The result ofTLC by mobile phase n-buthanol acetic acid water(4:1:5) andacetic acid 15% showed flavonoid compound.

Banana Peels (Musa paradisiaca L.) Extract as Phytoestrogen on Ovariectomized Mice Mammary Gland Development by Inducing c-Myc Expression

Pratama, Nanda Resa ( Cancer Chemoprevention Research Center (CCRC) Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta ) , Gilang, Yurista ( Cancer Chemoprevention Research Center (CCRC) Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta ) , Riata, Rita ( Cancer Chemoprevention Research Center (CCRC) Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta ) , Hermawan, Adam ( Cancer Chemoprevention Research Center (CCRC) Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta ) , Ikawati, Muthi ( Cancer Chemoprevention Research Center (CCRC) Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta ) , Meiyanto, Edy ( Cancer Chemoprevention Research Center (CCRC) Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta )

Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Hormone Replacement Therapy (HRT) is therapy for estrogen deficiency and post menopausal  syndromes,  but  high  cost  and  unwell-secured  therapy.  One  of  alternative therapy  is  the  usage  of  phytoestrogens.  The  banana  peel  contains  flavones,  flavonol, flavanone and polimethoxyflavone which are potential as phytoestrogen. The purpose of this study was to examine the estrogenic effect of banana peel extract (BPE) development of mammary gland of ovariectomized rats. Estrogenic effects was examined based on in vivo and in silico experiment. For in vivo experiment, female Sprague-dawley rats aged 50 days were ovariectomized. At 70 days of age, 12 rats were treated with BPE 500 mg/kgBB and 1000mg/kgBB, 5 rats were treated with estradiol 2g/day while others served as control were treated with CMC-Na 0.5% and sacrificed 2 weeks later. The base line ovariectomized rats and base line non-ovariectomized rats were sacrificed at 70 days of age. The in silico experiment examined by molecular docking between myricetin and estrogen receptor alpha (ER-α). The result of in vivo experiment showed that 1000 mg/kgBW BPE induced c-Myc expression  and  enhance  ovariectomized  rat  mammary  gland  development  significantly. Meanwhile, molecular docking showed that there are hydrogen bond interaction between bioactive compound in BPE and Estrogen Receptor (ER)-α but less powerfull than estrogen and ER-α interaction. In summary, BPE can act as an estrogen agonist,  resulting in the enhancement of c-Myc expression. 

Effect of Various Concentration of Vegetable Protein in Hair Mask on The Hair Texture

Helen, Ivony ( Fakultas Farmasi Unika Widya Mandala Surabaya ) , Hadinoto, Idajani ( Fakultas Farmasi Unika Widya Mandala Surabaya ) , Hadisoewignyo, Lannie ( Fakultas Farmasi Unika Widya Mandala Surabaya ) , Soegianto, Lisa ( Fakultas Farmasi Unika Widya Mandala Surabaya )

Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Effect of various concentrations of vegetable protein in hair mask on the hair texture including hair smoothness, hair shining, hair strength, and morphology of hair had been studied. Concentrations of vegetable protein are made 5%, 7.5%, and 10%. The hair mask was evaluated such as organoleptic, pH, viscosity, and allergy test. Hair mask used in six subjects that have damage hair for 8 times every 2 days. Then, the hair mask was evaluated such as hair smooth and hair shine that used tree trained panel and analyzed using non parametric method Q Cochran. Hair strength was evaluated using autograph  then  the  results  were    analyzes  using  ANOVA  test  (p<0.05),  while  hair morphology  was  evaluated  using  scanning  microscope  electron  (SEM).  The experimental  results  showed  that  vegetable  protein  in  hair  mask  increased  hair smoothness, hair strength, and morphology of damaged hair but did not give effect for hair shining. Various concentrations of vegetable protein (5%, 7.5%, and 10%) on hair mask given the same effect in hair texture (hair smoothness, hair strength, and hair morphology).

Anti-Inflammatory Activity of The Extract of Guava Leaves (Psidium guajava L) in The Rat (Rattus norvegicus L)

Weni, Linda ( Department of Biochemistry, Faculty of Medicine,YARSI University ) , Harliansyah, . ( Department of Biochemistry, Faculty of Medicine,YARSI University ) , Widayanti, . ( Department of Anatomy, Faculty of Medicine,YARSI University )

Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
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Abstract

Using of natural sources that have anti-inflammatory activity for the prevention and treatment of degenerative diseases began to be further explored. An investigation on the anti-inflammatory activity of the aqueous extract of guava leaves (Psidium guajava L.) from Sawangan, Depok on white male rats of Sprague-Dawley strain had been carried out on the carrageenan-induced  paw  edema  method.    To  examine  the  effect  of  guava  extract  on subcutan at different doses of 125, 250 and 500 mg/kg of body weight (BW).  Indometacine at dose of 10 mg/kg BW was used as a positive control. Observations were made during five hours with an interval of one hour. These results demonstrate that the percentage of inflammation or edema (% E) optimal at the 4th hour and then decreased at the 5th hour, while the percentage of optimal inhibition occurred at the 5th hour. Guava extract at 125, 250 and 500 mg/kg BW reduced inhibitory percentage activities by 40.81, 55.45 and 43.61% (p<  0.05)  respectively.  In  conclusion,  this  study  suggests  that  guava  extract  has  anti-inflammatory properties by decreasing edema level.

An Extract of Zingiber officinale and Piper retrofractum Combination and Its Effect to Cancer Cell Line

Ekowati, Heny ( Pharmacy Department, Medicine and Health Sciences Faculty, Universitas Jenderal Soedirman ) , Septiyaningsih, . ( Pharmacy Department, Medicine and Health Sciences Faculty, Universitas Jenderal Soedirman ) , Harwoko, . ( Pharmacy Department, Medicine and Health Sciences Faculty, Universitas Jenderal Soedirman )

Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
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Abstract

Chemotherapy may emerge sideeffect since it may treatinconveniently the synthesis of nucleic acids and proteins,both cancer cells or normal cells.Plants as a cancer therapy were expected to reduce this toxicityand side effects.Plants which used empirically for cancer therapy was Zingiber  officinale cv. Rubrum and Piper  retrofractum. This study was conducted to examine the cytotoxic activity of ethanolic extractc ombination of two plants in HeLa and T47D celllines.Zingiber  officinale cv.Rubrum,Piper  retro fractum and mixture (1:1)powdered then macerated with 96 % ethanol for 3x24 hours.Identification ofthe constituent that had potential anti cancer effect was usedTLC with silica GF254 as stationary phase,cytotoxic activity wasexamined by yellow MTT assay,the nanalyzed using probit.Apoptotic assay was performed by immunofluororescencemethod,using fluoro chromes ethidium bromide and acridineorange.The result showed that Zingiber officinalecv.Rubrumcontains terpenoids, while Piper  retrofractum contains alkaloids substance. The mixture showed cytotoxic activity against HeLa and T47D cell with IC50 33 and 53 6g/mL respectively.Theextract caused cytotoxic effect through apoptotic mechanism.

Cytotoxicity Assay From Fractions of Hedyotis corymbosa Extract Against Breast Cancer Cell Line T47D

Andriyani, Rina ( Research Center for Chemistry, Indonesian Institute of Sciences, ) , Risdian, Chandra ( Research Center for Chemistry, Indonesian Institute of Sciences, ) , Udin, Zalinar ( Research Center for Chemistry, Indonesian Institute of Sciences, )

Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Drug discovery for cancer medication is the most important effort that researcher do  at  this time. Indonesia bio diversities  have  possibility  as  a cancer medicine  sources. Finding a herbal medicine for cancer treatment is a first step to find a right cancer medicine in the future.This research has already completed for the earlier another research.Some fractions of Hedyotis corymbosa extract has been analyzed using Sulforhodamine B method with  UV wavelength  515 nm against T47D cell line, a human breast cancer. There are Hexane extract, Methylene chloride extract and Ethyl acetate extract,and give inhibitory concentration 50 (IC50) of 33.45 1g/ml, 54.59 1g/ml and 52.58 1g/ml, respectively. Ethanolic extract,  itself  has  IC50 of 61.57 1g/ml, whereas IC50 value of  Cisplatinum is 9.63 1g/ml. There is a difference between the ethanolic extracts with the other fraction.

Lymphocyte Proliferation Activity MTT -Test of Ethanolic Extract of Pasak Bumi Root (Eurycoma longifolia Jack) on Induced 7,12-Dimethylbenz[A] Antracene (DMBA) Female Sprague Dawley Rat

Falah, Lily Noor ( Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta ) , Yuliani, Sapto ( Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta )

Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Pasak bumi root (Eurycoma longifolia Jack) contains quasinoid. Quasinoid potentially has  antitumor/anticancer,  anti-parasitic,  and  immunostimulatory  activity.  The  aim  of  this research was to study the lymphocyte proliferation  activity MTT-test   of ethanol extract of  Eurycoma  longifolia  Jack  root  on  induced  by  7,12-dimethylbenz[a]  antracene  (DMBA) female Sprague Dawley rat. The test was done at 6 groups of 10  SD rat each. Each groups was  administered  orally  with  DMBA  (Group  I)    dose  of  20 mg/kg  bw,  corn oil  (Group  II), baseline (Group III), and  ethanol extract of pasak bumi root dose 12.6; 25.2; and 50.4 mg/kg bw (Group IV,V,and VI). Ethanol extract is given for 5 weeks. At the second week five rats were  sacrificed  to  isolate  the  lymphocytes  of  spleen  then  rats  are  injected  orally  with DMBA 2 times a week for 5 weeks. At the eight until twenty fourth week all groups were just  administered  by  aquades.  Measurement  of  spleen  relative  weight  and  isolation  of  the lymphocyte  spleen  were  done  at  second  week  and  the  last  of  experiment.  Lymphocyte proliferation  activity  were  measured  by  MTT-reduction  method.  Data  were  analyzed statistically  by  analyzes  of  variance  (anova)  continued  by      LSD  test  and  Kruskall  Wallis continued by Mann Whitney test. The result showed that limphocyte proliferation activity MTT of ethanol extract of E. longifolia root doses of 12.6; 25.2; and 50.4 mg/kg bw for 14 days prior to DMBA-induced are  339.35 ± 8.43;61.20 ± 6.27;  310.28 ±  6.81   and  dose 50,4 mg/kg bw after  DMBA-induced is 122.108 ±  11.426

Phytochemical Screening and Toxicological Evaluation Using Brine Shrimp Lethality Test (BSLT) of Some Fractions of Prasman Leaves (Eupatorium triplinerve V) Extract

Kumala, Shirly ( Faculty of Pharmacy, Pancasila University ) , Sapitri, Dwi Windi ( Faculty of Pharmacy, Pancasila University )

Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Prasman leaves (Eupatororium triplinerve V) was well documented to have anti cancer benefit in Indonesian traditional medicine history. However, there were noscientific studies including toxicological assessment on the planextract.The purpose of this study was to investigate thetoxicological effect of some fractions of Prasman leavesmethanol extract. Phytochemical screening by the Farnsworth method on powder and some fraction of the methanol extractwere conducted followed by toxicitytest using the “Brine Shrimp Lethality” test (BSLT) method. In the current study results, the phytochemical screening showed the presence of flavonoid, saponin, coumarin, tannin, steroidandvolatileoil.LC50ofthen-hexanefraction238.660g/mL,ethylacetatefraction 24.420g/mL,andn3butanol64.100g/mL.