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Articles by issue : Vol 9, No 3 (2015): September 2015
6
Articles
Genotype of Hepatitis B Virus Coinfection in Typhoid Patients

NURTJAHYANI, SUPIANA DIAN, HANDAJANI, RETNO

Microbiology Indonesia Vol 9, No 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

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Abstract

Typhoid fever can cause liver disorder and may result in complication. Studies revealed hepatic involvement in thypoid known as typhoid hepatitis. Our previous paper reported the existance of hepatitis B virus (HBV) coinfection in the serum of patients with typhoid using nested-polymerase chain reaction (PCR). Based on sequence divergence, HBV has been classified into 10 genotypes (A-J), which in prediction response and correlates with clinical outcome of chronic HBV infection. This study was conducted to determine the genotypes of HBV in typhoid patients coinfected with HBV in Tuban. Sera obtained from 5 typhoid patients positive HBV by nested PCR). Study was performed by direct sequencing BigDye V1.1 Terminator Cycle Sequencing kit and ABI Prism 310 Genetic Analyzer. Analys had been using the Genetix version 10 software to create the phylogenetic tree. Phylogenetic analysis showed 3 samples as genotype B and 2 two samples as genotype C.

Isolation and Identification of Bacteria from Raw Materials Contaminated by Rope-producing Bacteria

WIDYASARI, ANASTASIA ASRI ( Atma Jaya Catholic University of Indonesia ) , MAGDALENA, STELLA, LAY, BIBIANA WIDIYATI

Microbiology Indonesia Vol 9, No 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

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Abstract

Ropey bread is a bacterial spoilage condition of bread. The spoilage involved in ropey bread is primarily due to Bacillus subtilis. Several studies have shown that rope spoilage can be controlled using antimicrobial agents such as acetic acid, lactic acid, and quaternary ammonium cations (QACs/quats). This research consisted of five main steps: isolation, identification, confirmation, and molecular characterization of bacteria from raw materials contaminated by rope-producing bacteria, and antimicrobial test against rope-producing bacteria. The confirmation test was done in order to determine the rope-producing ability of isolates suspected as Bacillus sp. There were two different treatments in this test. In the first treatment, the inoculums were mixed with bread dough. In the second treatment, each slice of loaf was placed into a petri dish, uniformly soaked with inoculums. The first treatment did not show rope spoilage for all of the loaves, however, 6 of 11 loaves in the second treatment developed rope. The largest inhibition halos for antimicrobial test were produced by quats. This means quats is the strongest antimicrobial agent against rope-producing bacteria. The molecular characterization showed that all of the suspected isolates had 98–99% similarity to Bacillus subtilis.

Insecticidal Activities of Ethyl Acetate Extract of Indonesian Mangrove Fungus Emericella nidulans BPPTCC 6038 on Spodoptera litura

ABRAHAM, SILVA, BASUKRIADI, ADI, PAWIROHARSONO, SUYANTO, SJAMSURIDZAL, WELLYZAR

Microbiology Indonesia Vol 9, No 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

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Abstract

Mangrove fungi are known as sources of biological active compounds. The study and the report of secondary metabolites of mangrove fungi as insecticides is very limited in Indonesia. This study assess the insecticidal activities of ethyl acetate extract of Indonesian mangrove fungus Emericella nidulans BPPTCC 6038 against Spodoptera litura (Lepidoptera, Noctuidae) neonate larvae and pupae. The fungus E. nidulans BPPTCC 6038 was isolated from leaves of mangrove Rhizophora mucronata and identified based on ITS rDNA sequence data, with the GenBank accession number KP165435, and confirmed with morphological observation. This fungus strain was grown on malt extract broth for 14 days on rotary shaker at 65 rpm, and incubated at room temperature. Mortalities of S. litura were observed on larvae fed on artificial diet containing ethyl acetate extract of E. nidulans at concentrations of 625 – 5000 ppm. The lethal concentration of the extract which causes 50% mortality of larvae (LC50 value) was 1102.27 ppm. The other effects of fungus extract on S. litura were decrease in growth rate, longer larval period, inhibition on pupal development and absence in adult emergence. The HPLC analysis of extract showed that the crude extract contained three major compounds. This study provides evidence that the extract of E. nidulans possesses insecticidal activities against S. litura.

Determination of blaVIM and blaIMP Resistant Genes againts Meropenem of Pseudomonas aeruginosa Isolated from HCU Bronkopneumona Inpatients at Internal Medicine RSUP dr M Djamil Padang

NOVELNI, RINGGA, MARLINA, MARLINA, RAVEINAL, RAVEINAL

Microbiology Indonesia Vol 9, No 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

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Abstract

In this research, we aimed to detect blaVIM and blaIMP the resistant genes against Meropenem in Pseudomonas aeruginosa from sample of hospitalization patients at the Internal Medicine HCU of RSUP DR. M. Djamil Padang. Firstly, bacterial isolate of P. aeruginosa were isolated from the sputum samples of patients who suffered bronkopneumonia. The isolation were started with samples cultivation to the Cetrimide Agar media which was a selective media for P. aeruginosa. To determine the species of the bacteria, the identification using Gram staining, Triple Sugar Iron Agar (TSIA) test, citric test, urease test, Methyl Red/ Voges–Proskauer (MR/VP) test, and molecular marker of 16S rRNA genes have been conducted. The isolation and identification result showed that from 20 sputum samples of the patients there were just 10 (50%) samples were positively containing P. aeruginosa. From the P. aeruginosa isolates, the resistant genes against meropenem blaVIM and blaIMP were amplified using PCR. The result showed that all these P. aeruginosa isolates have positively genes encoding for Metallo-β-Lactamase (MBLs).

Optimization of Surfactin Production by Bacillus amyloliquefaciens MD4-12 using Response Surface Methodology

WIBISANA, AHMAD, SUMARYONO, WAHONO, SUDIRO, MIRAWATI, SUDARMONO, PRATIWI PUDJILESTARI

Microbiology Indonesia Vol 9, No 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

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Abstract

Surfactin is a lipopeptide biosurfactant that show potential biomedical application due to its activities such as antiviral, antibacterial, antifungi, anticancer, and antimycoplasma. Bacillus amyloliquefaciens MD4-12, isolated from oil-contaminated soil, produced promising yield of surfactin in McKeen medium. The production of surfactin was influenced by many fermentation process parameters such as carbon, nitrogen, minerals and also environmental conditions such as pH and agitation. Therefore, to obtain high yield of surfactin by Bacillus amyloliquefaciens MD4-12, optimization of process production was conducted in shake flask fermentation using response surface methodology. McKeen medium composition was used as basal medium.  Screening of the best carbon and nitrogen source were selected in preliminary experiments followed by selection of the influencing significant parameters on surfactin production using Plackett-Burman design. Selected parameters were optimized by central composite design and for the data analysis was used response surface methodology. The result showed that the optimum medium composition contained (g/L) 45.0 glucose, 6.33 urea, 1.0 monosodium glutamate, 1.85 MgSO4.7H2O, 0.4 KCl, 0.5 K2HPO4 and 0.5 mL trace elements. The surfactin yield at optimal condition was 1.25 g/L, increased 2.4 times compared to condition prior to optimization. 

Biosorption of Lithium Using Biofilm Matrix of Natural Microbial Consortium

KURNIAWAN, ANDI ( Brawijaya University ) , YAMAMOTO, TATSUYA

Microbiology Indonesia Vol 9, No 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

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Abstract

This study examined the biosorption of lithium using biofilm matrices of natural microbial consortiums collected from Lake Biwa, Japan. The characterization of the biofilm polymer as a suggested binding site of biofilm was also revealed in this study. The followings were observed as results of  this study: 1) biofilm has both negatively and positively charged sites; 2) lithium adsorption by biofilm matrix is a physicochemical process mainly promoted by the electrostatic interaction between the ion and the charged sites of biofilm polymers; 3) the adsorbing lithium ion promote the desorption of ions from biofilms through ion exchange mechanism; 4) biofilms components changed seasonally and seems to affect the ability of biofilm to adsorb ions. According the results of this study, natural biofilm may become a promising biosorbent in the biosorption of lithium ion.