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Articles by issue : Vol 7, No 1 (2013): March 2013
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Articles
Analysis of Bacterial Community Associated with Aaptos sp. from Rote and Seribu Islands

CHASANAH, EKOWATI ( Research and Development Center for Marine and Fisheries Product Processing and Biotechnology, Jalan KS Tubun, Petamburan 6, Jakarta, Indonesia ) , PATANTIS, GINTUNG ( Research and Development Center for Marine and Fisheries Product Processing and Biotechnology, Jalan KS Tubun, Petamburan 6, Jakarta, Indonesia ) , DEWI, ARIYANTI SUHITA ( Research and Development Center for Marine and Fisheries Product Processing and Biotechnology, Jalan KS Tubun, Petamburan 6, Jakarta, Indonesia ) , MARRASKURANTO, ENDAR ( Research and Development Center for Marine and Fisheries Product Processing and Biotechnology, Jalan KS Tubun, Petamburan 6, Jakarta, Indonesia ) , JANUAR, HEDI INDRA ( Research and Development Center for Marine and Fisheries Product Processing and Biotechnology, Jalan KS Tubun, Petamburan 6, Jakarta, Indonesia ) , STELLA, STELLA ( Faculty of Biotechnology, Universitas Katolik Atmajaya, Jalan Jenderal Sudirman 51, Jakarta 12930, Indonesia ) , SOKA, SUSAN ( Faculty of Biotechnology, Universitas Katolik Atmajaya, Jalan Jenderal Sudirman 51, Jakarta 12930, Indonesia ) , YOGIARA, YOGIARA ( Faculty of Biotechnology, Universitas Katolik Atmajaya, Jalan Jenderal Sudirman 51, Jakarta 12930, Indonesia )

Microbiology Indonesia Vol 7, No 1 (2013): March 2013
Publisher : Indonesian Society for microbiology

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Abstract

Aaptos sp. is a marine sponge that could produce bioactive compounds such as aaptamin, aaptosin, and isoaaptamin which have activities as antitumor, antimicrobial, and antiviral. Community of bacteria associated with the sponge might correlate with production of those bioactive compounds and be affected  by  water environment where the sponge grow. The presence of anthropogenic stressor such as pollutans might become a burden to the waters where the biota grown and could affect the microbial biodiversity in the sponge and its active metabolite produced. The objective of this research was to analyze bacterial community associated with Aaptos sp. from Rote Island and Seribu Islands, using T-RFLP method. The results showed that bacterial community associated with Aaptos sp. from both sampling sites shared 40.81% similarity in which they were dominated by the same bacteria class of Actinobacteria, Flavobacteria, α-proteobacteria, δ-proteobacteria, and γ–proteobacteria. The bacteria collected from Rote island  were more highly distributed and diverse than those from Seribu Islands. A total of 23 classes of microorganism were identified in Rote Island waters, while in Seribu Islands was 14 classes of microorganism. The presence of Actinobacteria and Proteobacteria in Aaptos sp., is allegedly involved in the production of secondary metabolites.

16S rDNA Typing of Salmonella Typhi Strains from Different Geographical Locations in Sumba Island East Nusa Tenggara Indonesia

AMARANTINI, CHARIS ( Biology Department, Universitas Kristen Duta Wacana, Jalan dr Wahidin Sudirohusodo 5-19, Yogyakarta 55224, Indonesia ) , BUDIARSO, TRI YAHYA ( Biology Department, Universitas Kristen Duta Wacana, Jalan dr Wahidin Sudirohusodo 5-19, Yogyakarta 55224, Indonesia )

Microbiology Indonesia Vol 7, No 1 (2013): March 2013
Publisher : Indonesian Society for microbiology

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Abstract

A total of thirteen isolates representative of Salmonella Typhi from different geographical locations in Sumba Island, East Nusa Tenggara, Indonesia were identified by 16S rDNA gene sequences. Bacterial DNA extraction was prepared by using a PurelinkTM Genomic DNA kit. The bacterial DNA and control were amplified using the specific primers for S. Typhi. These 16S rDNA gene sequence data were aligned with the corresponding available S. Typhi sequence and the reference organisms from the family Enterobacteriaceae from NCBI database by using the CLUSTAL X software. Phylogenetic trees were generated using the PHYLIP software package and the matrix of nucleotide similarity and nucleotide difference were generated by using the PHYDIT software. The results from the 16S rDNA analysis showed that the degree of similarity within these strains ranged from 99.13-100%. The percentage of sequence similarity between S. Typhi strains was very high (>99 %). Molecular phylogenetic analysis showed that all of the isolates formed a new center of diversity with S. Typhi ATCC 19430T as a reference strain. Based on these results, all of the tested strains belonged to species of S. Typhi suggested by their relatedness with the type strain of S. Typhi ATCC 19430T.

Bacterial Response after Exposure with Pure Metabolite Produced by Streptomyces sp. BL225 Isolated from Batanta Islands Leaf Litter

NURKANTO, ARIF ( Research Center for Biology Lembaga Ilmu Pengetahuan Indonesia ) , AGUSTA, ANDRIA ( Research Center for Biology Lembaga Ilmu Pengetahuan Indonesia ) , SJAMSURIDZAL, WELLYZAR ( Universitas Indonesia ) , JULISTIONO, HEDDY ( Research Center for Biology Lembaga Ilmu Pengetahuan Indonesia )

Microbiology Indonesia Vol 7, No 1 (2013): March 2013
Publisher : Indonesian Society for microbiology

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Abstract

The objective of this research was to investigate bacterial response after treatment with active metabolite produced by Streptomyces sp. isolated from Batanta Island. Minimum inhibitory concentration (MIC) values of four clinically tested bacteria (Escherichia coli, Bacillus subtilis, Staphylococcus aureus, and Micrococcus luteus) were successfully determined in this research using microdilution method. Leakages of nucleic acids and proteins from the tested microbes were detected using UV/VIS spectrophotometry method at 260 and 280 nm. Uracil leakage was analyzed using HPLC. Morphological changes of the bacterial cells were observed using scanning electron microscope (SEM). A Streptomyces isolate BL225 was identified based on the 16S rRNA gene sequence (1500 bp). When tested agains microbes, the MICs values of this compound were between 16-64 µg mL-1. The results indicated leakages of protein, nucleic acid and uracil from E. coli and B. subtilis cells after treatment with pure metabolite isolated from BL225. Treatment using metabolite from BL225 also caused morphological changes and damages of the target bacterial cell. BL225 had been identified as a strain that has closed relation to Streptomyces badius (98.9%).

Optimization Modeling of Ethanol Production from Shorgum bicolor grain: Comparison between Separate Hydrolysis Fermentation and Simultaneous Saccharification Fermentation

CHRISNASARI, RUTH ( Department of Biology, Faculty of Biotechnology, Universitas Surabaya, Jalan Raya Kalirungkut, Surabaya 60292, Indonesia ) , SUSETYO, DAMIATI HARTINI ( Department of Biology, Faculty of Biotechnology, Universitas Surabaya, Jalan Raya Kalirungkut, Surabaya 60292, Indonesia ) , SUGIANTO, ADRIAN PRATAMA ( Department of Biology, Faculty of Biotechnology, Universitas Surabaya, Jalan Raya Kalirungkut, Surabaya 60292, Indonesia ) , PANTJAJANI, TJANDRA ( Department of Biology, Faculty of Biotechnology, Universitas Surabaya, Jalan Raya Kalirungkut, Surabaya 60292, Indonesia )

Microbiology Indonesia Vol 7, No 1 (2013): March 2013
Publisher : Indonesian Society for microbiology

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Abstract

Two different process configurations, simultaneous saccharification and fermentation (SSF), and separate hydrolysis and fermentation (SHF), were compared for ethanol production from Shorgum bicolor grain. Optimization modeling for glucoamylase and Zymomonas mobilis concentration in both of SSF and SHF were carried out to obtain optimal concentration of ethanol production. The optimum condition was achieved using 0.021 % (v/v) of glucoamylase, and 30.19% (v/v) of Z. mobilis for SHF. In contrast, the optimum condition for SSF was 0.021 % (v/v) of glucoamylase and 17.51% (v/v) of Z. mobilis. The model predicted SHF processing to be superior. The superiority of SHF over SSF was confirmed experimentally, the result showed ethanol yield of SHF was 134.80 g L-1 and ethanol yield of SSF was 115.66 g L-1 after 72 h incubation time. A high similarity was observed between the predicted and experimental results, demonstrating the accuracy of the model.

The Effect of a Mixed-Starter Culture of Lactic Acid Bacteria on the Characteristics of Pickled Orange-Fleshed Sweet Potato (Ipomoea batatas L.)

YULIANA, NETI ( Department of Agricultural Product Technology (THP), Agriculture Faculty, Universitas Lampung, Jalan Sumantri Brojonegoro No 1, Bandar Lampung 35145, Indonesia ) , NURDJANAH, SITI ( Department of Agricultural Product Technology (THP), Agriculture Faculty, Universitas Lampung, Jalan Sumantri Brojonegoro No 1, Bandar Lampung 35145, Indonesia ) , MARGARETA, MIKA ( Department of Agricultural Product Technology (THP), Agriculture Faculty, Universitas Lampung, Jalan Sumantri Brojonegoro No 1, Bandar Lampung 35145, Indonesia )

Microbiology Indonesia Vol 7, No 1 (2013): March 2013
Publisher : Indonesian Society for microbiology

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Abstract

In this study, fermentation process was carried out on orange-fleshed sweet potato cubes to produce sweet potato pickle using a mixed culture of Lactobacillus plantarum and Leuconostoc mesenteroides at 30 °C over 12 days period. Spontaneous fermentation was also performed as a control. Samples were withdrawn at various time intervals for analyses of reducing sugar content, total number of lactic acid and non-lactic acid bacteria, lactic acid concentration, pH, and sensory attributes. The results showed that using a mixed culture of L. plantarum and L. mesenteroides could greatly reduce contamination of non-lactic acid bacteria, retaining low amount of reducing sugar, rapidly producing lactic acid and consequently decreasing pH value of the pickle, as well as giving better sensory score. After 12 d of fermentation, sample of pickle inoculated with mixed culture showed the following characters: total lactic acid content 0.5%, total lactic acid bacteria 8.46 log10 CFU mL-1, total non-lactic acid bacteria 1 log10  CFU mL-1, total reducing sugar 0.84 g L-1, texture 64.92 mm 50 g-1 s-1, and hedonic sensory score for both taste and aroma 4 (like) in a scale of 5. These results indicated the potential ability of the mixed culture of lactic acid bacteria to improve the quality of the pickle fermented spontaneously.