cover
Filter by Year
Articles by issue : Vol 5, No 4 (2011): December 2011
6
Articles
Stability of Viable Counts of Lactic Acid Bacteria during Storage of Goat Milk Soft Cheese

RAHAYU, WINIATI PUDJI ( Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Institut Pertanian Bogor ) , KUSNANDAR, FERI ( Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Institut Pertanian Bogor ) , PRAYITNO, WIDYA EKA ( Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Institut Pertanian Bogor )

Microbiology Indonesia Vol 5, No 4 (2011): December 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Original Source | Check in Google Scholar | Full PDF (87.392 KB)

Abstract

The use of goat milk is limited in Indonesia due to lack of good milking practices resulted in disliked goaty smell. One of the method to eliminate this off flavor is by processing the goat milk into soft cheese. The aim of this research was to study the stability of viable starter lactic acid bacteria cultures  (Lactobacillus acidophilus FNCC-0051 and L. casei FNCC-0090) during storage of goat milk soft cheese.  Three batches of goat milk soft cheeses were produced with different starter cultures L. acidophilus FNCC-0051 (5.0 x 106 cfu mL-1); L. casei FNCC-0090 (5.0 x 106 cfu mL-1); and the mixture of L. acidophilus FNCC-0051 (2.5 x 106 cfu mL-1) and L. casei FNCC-0090 (2.5 x 106 cfu mL-1). The goat milk cheeses had white color and soft. The viable lactic acid bacteria in the goat milk soft cheese reached 109 cfu g-1, which was stable for  8 weeks at 5 °C. Panelists liked goat milk soft cheeses, especially in term of its aroma. The specific aroma produced could mask the disliked goaty smell.

Selection of Yeasts Antagonists as Biocontrol Agent of Mango Fruit Rot caused by Botryodiplodia theobromae

SUGIPRIHATINI, DWI ( Balai Besar Uji Standar Karantina Pertanian ) , WIYONO, SURYO ( Department of Plant Protection, Faculty of Agriculture, Institut Pertanian Bogor ) , WIDODO, . ( Department of Plant Protection, Faculty of Agriculture, Institut Pertanian Bogor )

Microbiology Indonesia Vol 5, No 4 (2011): December 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Original Source | Check in Google Scholar | Full PDF (164.9 KB)

Abstract

Fruit rot caused by Botryodiplodia theobromae is one of the most important post harvest disease of mango in Indonesia. Study on biological control on the disease is required to develop environmentally-sound control technology.  The research objectives were to study the potency of yeasts in controlling post harvest mango disease i.e. fruit rot caused by B. theobromae and mechanism involve in the biocontrol. Total yeast isolates used for screening were twenty one, four from collection of Plant Clinic of Institut Pertanian Bogor, and twenty one isolated from healthy mango skin. All of yeast isolates were characterized and identified using BIOLOG. Bioassay on antagonistic activity of yeasts against fruit rot, in-vitro dual culture test and chitinolytic activity were carried out.  Cryptococcus albidus var. aerius WSW1, Pichia guilliermondii K1, and Debaryomyces hansenii K12 were the three most effective antagonistic yeasts against B. theobromae with effectiveness of 70.83%, 45.83%, 37.50% respectively. In vitro bio-assay showed that C. albidus var. aerius WSW1, C. albidus WSW2, C. albidus K6, C. terreus YSW1, Candida edax OSW1, Candida edax K13, and Cryptococcus luteolus K2 had high antibiosis activity.  Biocontrol activity of tested yeasts against fruit rot of mango did not correlate to its antibiosis and chitinolytic activity

Isolation and Identification of Emestrin from Emericella nidulans and Investigation of Its Anticancer Properties

NURSID, MUHAMMAD ( Research Center for Marine and Fisheries Product Processing and Biotechnology, Kementerian Kelautan dan Perikanan ) , CHASANAH, EKOWATI ( Research Center for Marine and Fisheries Product Processing and Biotechnology, Kementerian Kelautan dan Perikanan ) , MURWANTOKO, . ( Fisheries Department, Faculty of Agriculture, Universitas Gadjah Mada, ) , WAHYUONO, SUBAGUS ( Faculty of Pharmacy, Universitas Gadjah Mada )

Microbiology Indonesia Vol 5, No 4 (2011): December 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Original Source | Check in Google Scholar | Full PDF (1271.7 KB)

Abstract

The research to isolate, identify and investigate of anticancer properties of active compound produced by Emericella nidulans marine fungus has been done. Active compound was isolated from mycelium extract of the  fungus. The molecular formula of active compound was established as C27H21N2O10S2 by LC-ESI-ToF-MS (m/z 597.1105 [M – H]-. Elucidation of molecular structure using FT-IR, LC-ESI-ToF-MS, 1H-NMR, 13C-NMR, and DEPT 135˚ showed that the active compound was emestrin. Emestrin was found to have cytotoxic effect against T47D, HepG2, C28, and HeLa but it was not too toxic against Vero cells with IC50 value of 1.8 µg mL-1, 4.2 µg mL-1, 2.6 µg mL-1, 13.8 µg mL-1, and 260.9 μg mL-1, respectively. Base on the cell cycle analysis by using flow cytometry, emestrin treatment at concentration of 1.0 μg mL-1 induced cell-cycle arrest in G0/G1 phase whereas at concentration of 3.0 μg mL-1, a sub-population of cells (sub G1) appeared. The apoptosis assay by using Annexin-V-FLUOS revealed that most of T47D cell treated with the compound at 1.0 and 3.0 μg mL-1 underwent apoptosis (83.6% and 92.6%, respectively. This anticancer activity of emestrin may be related to the unique of the epithiodioxopiperazine moiety with internal disulphide bond of this compound.

Characterization and Phylogenetic Analysis of Soybean Rhizobial Strains from Java and Sumatra

WALUYO, SETIYO HADI ( Center for the Application of Isotopes and Radiation Technology, National Nuclear Energy Agency (BATAN) )

Microbiology Indonesia Vol 5, No 4 (2011): December 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Original Source | Check in Google Scholar | Full PDF (357.037 KB)

Abstract

Twenty-seven and twenty-four soybean rhizobial isolates from Java and Sumatra, respectively,were characterized. Based on cross-inoculation,  eight isolates from Java and nine from Sumatra could be grouped as soybean specific rhizobial species , while 19 isolates from Java and 15 from Sumatra were  promiscuous. ARDRA of intergenic spacer region of 16S-23S rDNA showed that the isolates from Java were different from those from Sumatra. Six soybean specific isolates from Java and one from Sumatra were in the same cluster with the reference strain, Bradyrhizobium japonicum USDA 110, thus could be classified as B. japonicum. One soybean specific isolate from Java’s has a distinct position, while the other soybean specific isolate from Java was placed in another group dominated by isolates from Sumatra. The nineteen promiscuous isolates from Java were clustered in a different group. This group, together with the isolate with distinct position and the other group that were dominated by isolates from Sumatra, were distinct from B. japonicum USDA 110. Therefore it is tempting to speculate that they represent indigenous soybean rhizobial strains. Based on complete sequencing of the amplified 16S rDNA of 21 selected isolates, these isolates could be divided into three groups consisting of twelve Bradyrhizobium elkanii, eight Bradyrizobium japonicum and one Sinorhizobium fredii. Most of the B. elkanii strains were isolated from acid soils at Sitiung, West-Sumatra, while only two isolates were obtained from Java. Four isolates from Java, two isolates  from Sitiung, and two isolates from Bukit Tinggi were identified as B. japonicum. One isolate from Java with a distinct position on the ARDRA was identified as S. fredii.

Photosynthesis of Periphyton and Diffusion Process as Source of Oxygen in Rich-Riffle Upstream Waters

PRATIWI, NIKEN TUNJUNG MURTI ( 1Department of Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, Institut Pertanian Bogor ) , HARIYADI, SIGID ( 1Department of Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, Institut Pertanian Bogor ) , TAJUDIN, RIFKI ( 1Department of Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, Institut Pertanian Bogor )

Microbiology Indonesia Vol 5, No 4 (2011): December 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Original Source | Check in Google Scholar | Full PDF (234.612 KB)

Abstract

Most of dissolved oxygen content in stream or river water is obtained from the process of photosynthesis and diffusion.  Photosynthesis process in running water is performed by autotrophic organisms, especially community of attached micro-algae or periphytic micro-algae that live attached to stone or other substrates, while intensive diffusion process occurred as water flows.  Rich riffle upstream waters are characterized by stony bottom and high current.  The role of the periphyton photosynthesis result and diffusion process along the water current was examined by this research. A field experimental observation was carried out at the upstream of Cisadane River (600m asl) with two different natural light conditions, the light or exposed (24630-104240 Lux) and shadowy (11120-65300 Lux) conditions.  The actual dissolved oxygen contents in the area with the two conditions are relatively similar; 7.3 (light) and 7.0 mg L-1 (shadowy).  Oxygen supports were significantly different at the light and shadowy conditions, whether from diffusion process 71.71% and 79.37%, respectively, or from periphyton photosynthesis, 21.73% and 15.30%, respectively. The compositions of periphytons living in the two conditions were similar; mostly composed by group of Diatom, with the same dominant species of Thalassiothrix sp.  The difference in periphyton growth was shown by its density.  In the light condition, the Diatom tends to grow at higher density in comparison to the shadowy condition. The role of periphyton to support oxygen in upstream waters is light dependent.  The higher the light intensity (Photosynthetic Active Radiation ranged), the higher the support of oxygen will be.

Selection of Carbon and Nitrogen Source for 8-Hydroxy 9, 12-Octadecadienoic Acid Production using Endophytic Fungi Curvularia lunata BioMCC FE-00283

PRABANDARI, ERWAHYUNI ENDANG ( Graduated School of Agriculture Industrial Technology, Bogor Agricultural Institute ) , IRAWADI, TUN TEDJA ( Graduated School of Agriculture Industrial Technology, Bogor Agricultural Institute ) , SUMARYONO, WAHONO ( Deputy of Technology of Agroindustry dan Biotechnology-BPPT ) , SYAMSU, KHASWAR ( Graduated School of Agriculture Industrial Technology, Bogor Agricultural Institute )

Microbiology Indonesia Vol 5, No 4 (2011): December 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Original Source | Check in Google Scholar | Full PDF (84.109 KB)

Abstract

Hydroxyoctadecadienoic acid (HODE) is one of hydroxy fatty acids that has anticancer activity.  HODE was previously produced by chemical synthesis or bioconversion from linoleic acid. This is the first paper reported production of HODE by Curvularia lunata an endophytic fungi of Cibotium barometz. Various carbon and hydrogen sources have been tested for their effects on the production of  HODE by C. lunata. Glucose, lactose, maltose, xylose, and sucrose were used as carbon sources, while yeast extract, monosodium glutamate, urea, and NH4Cl were used as nitrogen sources. Fermentation was done using 100 ml medium in 250 ml Erlenmeyer flask at 150 rpm, 28 oC for 10 days.  HODE products were analyzed by high pressure liquid chromatography using C18 coloumn and eluted by gradient system of acetonitril-water from 15% to 100%. Glucose  and monosodium glutamate were found to be the best carbon and nitrogen source.  The optimum concentration of glucose and monosodium glutamate for the production of HODE were 10 mg L-1 and 12 mg L-1 respectively.