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Articles by issue : Vol 11, No 4 (2017): December 2017
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Articles
The Utilization of Modified Cassava Flour (Mocaf) Industry Waste and Peat as Carrier of Nitrogen-Fixing Bacteria and Phosphate Solubilizing Bacteria Inoculant

ROSARIASTUTI, RETNO ( Sebelas Maret University ) , SUMANI, SUMANI, SUPRIYADI, SUPRIYADI, NURSETYAWAN, MUHAMMAD ARDIAN, DANISWARA, PRAMUSITA YOGA

Microbiology Indonesia Vol 11, No 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

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Abstract

Fertilizer demand in Indonesia increase for increasing agricultural production.Biofertilizer is organic fertilizer with the addition of specific microorganisms which are expected to reduce the amount of inorganic fertilizer. Carrier material plays an important role in maintaining the viability and storage period. Peat is used as a biofertilizer carrier for  a long time. Solid waste of Modified Cassava Flour (Mocaf) which is the remainder of mocaf industry has great potential as a carrier material of good biofertilizers,  because of its nutrient content. The aim of this study was  determining the potential of mocaf solid waste and its combinations with peat as the carrier in supporting the growth of Nitrogen-fixing Bacteria (NFB) and Phosphate Solubilizing Bacteria (PSB) during the incubation  of microorganisms. The experiment was conducted at the Laboratory of Soil Biology and Biotechnology, Faculty of Agriculture of Sebelas Maret University (UNS) using completely randomized design (CRD) with two factors of a carrier and incubation time as the experimental design. There  were  three  types of  carrier  which  have different  combination. The base material were solid  waste  of  Mocaf  industry   and  peat.  All materials  of carrier  were  mixed and sterilized, than inoculated by Nitrogen- Fixing  Bacteria (NFB)  and  Phosphate Solubilizing  Bacteria (PSB) and incubated  for  60  days. The  growth of  bacterias were analyzed  every 15  days  and  the  chemical composition  of  carrier  were  analyzed  at  the begin and the  end of  research   (incubation).    The results indicated that the incubation time significantlyaffected viability of NFB and PSB. Until 60th day incubation time, still showed the increasing growth of NFB and PSB.

Potential Degradation of SARA (Saturated, Aromatics, Resinics, Asphaltenes) Fractions of Crude Oil by Reservoir Indigenous Bacteria from South Sumatera

ASTUTI, DEA INDRIANI, PURWASENA, ISTY ADHITYA, ADITIAWATI, PINGKAN, SANI, INDIANI, ARIADJI, TUTUKA, ABQORY, M. H.

Microbiology Indonesia Vol 11, No 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

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Abstract

MEOR (Microbial Enhanced Oil Recovery) technology utilizes metabolic activity of microorganisms such as degradation of hydrocarbon fractions which alters oil characteristics to facilitate and increase oil recovery from reservoir. This research focused on isolation of indigenous hydrocarbonoclastic bacteria that were capable of degrading SARA (Saturated, Aromatic, Resinic, Asphaltenes) fractions of crude oil to be utilized in MEOR. Sequential isolation of hydrocarbonoclastic bacteria were conducted using Nutrient Broth and Stone Mineral oSalt Solution medium supplemented with 2% (v/v) crude oil and 0.1% (w/v) yeast extract and incubated at 50C, 120 rpm agitation. Isolates retrieved were screened based on its activity to degrade crude oil, indicated by resazurin assay result. Physical and chemical characteristics of crude oil altered by selected isolates were observed using column chromatography, biometric test, GC-MS analysis, IFT and viscosity measurements. Isolates with the best degradation activity were identified with 16S rRNA gene sequencing. Among thirty-one bacterial isolates obtained from sequential isolation, six isolates exhibited high oil degrading ability. SARA assay showed degradation activity of those isolates to each of SARA fraction were around 6-70%.  This degradation was followed by significant CO production ranging from 2000-4000 mg (value of p<0.05). Microbial 2 degradation activity exhibited changes in chemical and physical characteristics of hydrocarbons showed by changes in composition of SARA fraction, decreased viscosity and IFT of crude oil 17-31%. This research identified three isolates with the best hydrocarbon fraction degrading ability were identified as different strain of Bacillus licheniformis and confirmed their high potential to be utilized in MEOR technology.

ITA REGISTRATION FORM AND BACK COVER

Helianti, Is ( 1. BPPT 2. Indonesian Society for Microbiology )

Microbiology Indonesia Vol 11, No 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

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Abstract

Isolation and Identification of Ethanol and Glucose Tolerance Yeasts Strain from Tacca leontopetaloides

UTAMA, GEMILANG LARA, SUGANDI, WAHYU K., LEMBONG, ELAZMANAWATI, SURYADI, EDY

Microbiology Indonesia Vol 11, No 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

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Abstract

The research aimed to isolate and characterize indigenous yeast strain from Tacca leontopetaloides with respect to the ethanol and glucose tolerance ability. Research done experimentally and the data analyzed descriptively. Yeasts isolated from 1g Tacca leontopetaloides grown at modified Potato Dextrose Agar/PDA (Oxoid Ltd.) with 3% Yeasts Extract/YE (Kraft Foods) and 10 ppm amoxicillin addition. Yeasts-like colony was tested in the ability to tolerate ethanol and glucose contents by grown on modified Nutrient Broth/NB (Oxoid Ltd.) with 3% Yeasts Extract/YE (Kraft Foods) and 10 ppm amoxicillin then added with glucose monohydrate (10%, 20%, 30%) or ethanol (10%, 20%, 30%) and incubated for 72h at ambient (23-28°C). Optical density (OD) was read for UV absorbance at 600 nm using UV-Vis spectrophotometer every 24h until 72h. The strain of best isolate with the ability to tolerate high ethanol and glucose contents were identified by the sequence analysis of ITS (Internal Transcribed Spacer) region using primers ITS1 (5′-TCCGTAGGTGAACCTGCGG-3′) and ITS4 (5′-TCCTCCGCTTATTGATATGC-3′). The sequencing was performed at Macrogen Inc. (Seoul, South Korea), and the sequences was compared with the GenBank database using BLAST (Basic Local Alignment Search Tools) algorithm (http//:www.ncbi.nlm.nih.gov/BLAST/). Results shown there are four yeasts-like isolate and the TK1 isolate showed the best ethanol tolerance ability with highest OD at 30% ethanol concentration (0.486) and the highest OD at 30% glucose concentration (1.732). The species identification identified the TK1 isolate as 99% identical with Candida natalensis (ITS1) and 100% identical with Candida quercitrusa (ITS4).

Identification and Characterizations of Potential Indigenous Endophytic Bacteria which had Ability to Promote Growth Rate of Tomato and Biocontrol Agents of Ralstonia solanacearum and Fusarium oxysporum fsp. solani

YANTI, YULMIRA, WARNITA, WARNITA, REFLIN, REFLIN, BUSNIAH, MUNZIR

Microbiology Indonesia Vol 11, No 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

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Abstract

Among Plant Growh Promoting Rhizobacteria (PGPR) groups, endophytic bacteria considered as one of the options to control vascular wilt disease because of its ability to live and colonized internal roots of plants without causing any damages. Our previous research had screened 9 isolates which had best ability to promote growth rate and increase yields of tomato and biocontrol agents of R. solanacearum and Fusarium oxysporum f.sp solani in planta condition. In order to know its abilities, those isolates need to be characterized. This research purposedto characterize those isolates abilities to produce IAA, phosphate solubilizing, siderophore production, cyanide production, NH3 production, and ability to colonize endophytically and identified the isolates using 16S rRNA. Result shown that all isolates can produce IAA, where TLE1.1 produce highest IAA concentration (42.5 ppm). Isolates E1AB1.3, TLE 1.1 and TLE2.2 can dissolved phosphate. None of the isolates produced HCN and NH3. Only TLE 2.3 isolate can produce siderophore. All of 9 isolates were identified using 16S rRNA gene using 27F and 1492R primers. All isolates were identified as different species, i.e. Bacillus toyonensis strain BCT-7112 (EPL1.1.3), Serratia nematodiphila strain DZ0503SBS1 (TLE2.3), Bacillus anthracis strain ATCC 14578 (EPL1.1.4), Bacillus cereus ATCC 14579 (TLE1.1), Bacillus cereus strain JCM 2152 (SNE2.2), Enterobacter cloacae subsp. dissolvens strain ATCC 23373 (E1.AB1.2), Serratia marcescens strain NBRC 102204 (E1AB2.1), Klebsiella michiganensis strain W14 (TLE2.2), and Chryseobacterium rhizoplanae strain JM-534 (KLE3.3).

Molecular Identification of Thermally-tolerant Symbiotic Dinoflagellates from Hard Coral (Scleractinia) in Biawak Island, Indonesia

RORIRIS, EVINA TAMI, AGUNG, MOCHAMAD UNTUNG KURNIA, ASTUTY, SRI, MULYANI, YENI

Microbiology Indonesia Vol 11, No 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

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Abstract

Symbiodinium is phototrophic dinoflagellates that occur as endosymbionts in various marine invertebrates and protists, forming mutualistic symbiosis with their hosts. The composition of Symbiodinium populations may also play an important role in the tolerance or sensivity of corals towards bleaching. Therefore, this research aims to identify thermally-tolerant Symbiodinium of scleractinian corals in Biawak Island using molecular techniques. Sampling was carried out from Acropora sp and Porites sp, from the depth of 3-5 meters. Symbiodinium was TMisolated through metagenomic approach using ZR Soil Microbe DNA Kit and 28S nrDNA gene was amplified TMusing Polymerase Chain Reaction (PCR). The resulted sequences were processed using BioEdit software and TMMEGA 5.2 for phylogenetic tree construction. The results indicated that isolates of ZX-ACP-ST.1 and ZXPORITES-ST.3 have similiarity to registered sequences of Symbiodinium thermophilum with identity value of TM99% and 98%. BLAST analysis of ZX-PORITES-ST.2 isolate indicated high identity (99%) to sequences of Symbiodinium sp. clade C while ZX-PORITES-ST.3 isolate has 99% similiarity to Symbiodinium sp. CG8. Phylogenetic analysis using UGPMA method showed that all isolates had a very close relationship and thought to have come from Symbiodinium clade C.