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Articles by issue : Vol 11, No 3 (2017): September 2017
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Articles
Oil Recovery Test Using Bio surfactant of Halo tolerant Bacteria Brevundimonas diminuta and Bhurkholderia glumae at variation of NaCl Salt Concentrations

YUDONO, BAMBANG, SAID, MUHAMMAD, ESTUNINGSIH, SRI PERTIWI, KARIMA, AULIA

Microbiology Indonesia Vol 11, No 3 (2017): September 2017
Publisher : Indonesian Society for microbiology

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Abstract

Oil recovery test has been done by using crude biosurfactant from Brevundimonas diminuta and Bhurkholderia glumae indigenous halo tolerant bacteria with the vatiation of NaCl salt concentration 0; 1.5; 3; 4.5; 6; and 7.5%. Oil recovery test was obtained by determining % TPH (Total Petrolem Hidrocarbon). The sample concentration was 28.19% TPH, it was extracted by using biosurfactant of  Brevundimonas diminuta and Bhurkholderia glumae bacteria, the optimal salitnity conditions were  at 3, 4.5% salt concentrations with the value oil recovery as much as 50.41, 69.97 % respectively. Oil components which extraction by biosurfactant were analyzed by using GC-MS (Gas Chromatography-Mass Spectrophotometry). The result from analyzes GC-MS could be concluded that bacteria Brevundimonas diminuta could dissolve hydrocarbon compounds short chain carbon atom at fraction <C10–C14 and long chain carbon atom at fraction >C22. <C10, C11-C14dan C15-C17  and Bhurkholderia glumae could dissolve hydrocarbon compounds short chain carbon atom at fraction <C10–C14 and long chain carbon atom at fraction >C22 according to the retention time.

The Dynamic Growth and Chemical Change of Mixed Cultures Inoculation on Tapioka Fermentation

KUSTYAWATI, MARIA ERNA, RANGGA, AZHARI, SETYANI, SRI

Microbiology Indonesia Vol 11, No 3 (2017): September 2017
Publisher : Indonesian Society for microbiology

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Abstract

Lactobacillus plantarum and Saccharomyces cerevisiae possess several of extracellular and intracellular of enzymes beneficial to cassava fermentation. Tapioka (cassava starch) has limited uses in food industries due to its low pasting properties, therefore, biomodification by the use of fermentation is needed. The research was aimed to monitor the growth of Saccharomyces cerevisiae and L. plantarum during tapioca fermentation, and to evaluate the chemical change, of the fermented tapioka. Mixed cultures was inoculated at the designed concentration into tapioca suspension and incubated at room temperature (30±2oC) in facultative aerobic condition for 0, 24, 48, 60, 72, 96, 120, and 144 h. The growth change of S.cerevisiae and L. plantarum was monitored, and the change of pH, residual sugar, and starch granule was investigate. The result showed that S. cerevisiae had longer lag phase as well as stationary than L. plantarum was; nevertheless, they both reached log phase at the same time. Co-inoculated mixed cultures did not affect the change on pH and reducing sugar but increased pronouncely protein content at stationary period. Besides, there was sign of erosion to the structure of cassava starch granules which was an indication of changes in the pasting property of the cassava starch. 

Antibacterial Potentiality Testing of Pineapple Core Extract (Ananas comosus (L.) Merr) Against Methicillin-resistant Staphylococcus aureus (MRSA) with Vancomycin Control

PUTRA, BOBY PRATAMA ( Faculty of Medicine, Universitas Airlangga, Indonesia ) , INDIASTUTI, DANTI NUR, KUSUMANINGRUM, DEBY

Microbiology Indonesia Vol 11, No 3 (2017): September 2017
Publisher : Indonesian Society for microbiology

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Abstract

Staphylococcus aureus is one of major pathogens causing serious infection. Penicillin antibiotic is one of therapies against Staphylococcus infection. However, inadequate and irrational use of antibiotic causes resistance and emerges incidence of methicillin-resistant Staphylococcus aureus (MRSA). Herbal medicine from pineapple core extract is hopefully can reduce the incidence of antibiotics resistance. This research was conducted to investigate the antibacterial activity of pineapple core extract against MRSA.This research is true experimental with post-test controlled group design. Pineapple core was extracted by maceration method. Ethanol extract of pineapple core is dissolved with sterilized water and obtained concentration of 750, 500, 250, 187.5, 125, and 62.5 mg/ml. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by broth dilution test with five replications. Vancomycin was used as control group. MIC was observed visually by comparing turbidity of solutions after incubation at 37oC for 24 hours. Then these solutions were cultured on nutrient agar plates at 37oC for 24 hours. MBC was observed visually by inspecting the presence of bacterial colonies growth.The Minimum Inhibitory Concentration (MIC) could not be determined due to no turbidity changes. Vancomycin cannot be used for determining MIC. Cultures on nutrient agar plates had no colonies growth in concentrations of 750 and 500 mg/ml. Thus, the Minimum Bactericidal Concentration (MBC) was 500 mg/ml.Pineapple core extract contains bromelain, flavonoid, saponin, and tanin, which have antibacterial effect. In summary, pineapple core extract has antibacterial effect against methicillin-resistant Staphylococcus aureus (MRSA) with MBC of 500 mg/ml.

ITA REGISTRATION FORM AND BACK COVER

Helianti, Is ( 1. BPPT 2. Indonesian Society for Microbiology )

Microbiology Indonesia Vol 11, No 3 (2017): September 2017
Publisher : Indonesian Society for microbiology

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Abstract

Performance Optimization of Microbes from Shrimp Pond Sediment by Adding EM4 In Nitrification Process for the Treatment of Wastewater Containing High Ammonia Concentration

AMBARSARI, HANIES ( Badan Pengkajian Penerapan Teknologi (BPPT) ) , HARAHAP, MUHAMMAD RAHMADI

Microbiology Indonesia Vol 11, No 3 (2017): September 2017
Publisher : Indonesian Society for microbiology

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Abstract

 In liquid wastes, especially domestic wastewater, many organic substances are mixed causing water quality degradation, one of them is ammonia. Liquid wastes containing ammonia can be treated using an activated sludge system. One of the active sludge that can be used is shrimp pond sediments. This experiment investigated the performance of microbes in shrimp pond sediments with the addition of EM4 in nitrification process for the treatment of wastewater with high ammonia concentration in a 8 L batch reactor capacity. The results show that the addition of shrimp pond sediment as the active sludge can remove high ammonia level almost completely and there is known interaction between time and variation of shrimp pond quantity (p value <0,05) to the decreasing of ammonia level. Efficiency of decreasing the concentration of ammonia up to 100% can be reached on the 15th day in each treatment. The addition of EM4 can shorten the decreasing of ammonia level by 50%. Keywords: Nitrification, Ammonia, Shrimp Pond Sediment, EM4, Activated Sludge

In Vitro Phytochemical and Inhibitory Potential Test of Bawang Hutan Bulb Extract (Eleutherine palmifolia) on Vibrio harveyi

MUNAENI, WAODE ( Institut Pertanian Bogor ) , PARIAKAN, ARMAN ( Halu Oleo University ) , ABIDIN, LAODE BAYTUL ( Halu Oleo University ) , YUHANA, MUNTI ( Bogor Agricultural University )

Microbiology Indonesia Vol 11, No 3 (2017): September 2017
Publisher : Indonesian Society for microbiology

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Abstract

The objectives of this study were to analyze phytochemical content of bawang hutan bulbs extract (Eleutherine palmifolia) and to test the inhibitory potential of bawang hutan bulbs extract on the growth of Vibrio harveyi bacteria at different doses. This study was conducted in March-May 2017 in Testing Laboratory of Fisheries and Marine Science Faculty of Halu Oleo University and Laboratory of Fish Health of Aquaculture Department of Fisheries and Marine Science Faculty and Laboratory of Biopharmaca of Bogor Agricultural University. Test parameter included: (1) Phytochemical test through the method of color visualization, (2) Inhibitory potential test using two methods namely agar diffusion and co-culture. Treatment of dose consisted of positive control/K+ (Chloramphenicol 30 mg/ml), negative control/K- (Sterile Aquadest) and treatment of extract included A (20 mg/ml), B (40 mg/ml), C (60 mg/ml), D (80 mg/ml). Qualitatively, result of phytochemical test showed that bawang hutan bulbs extract contained flavonoid, tannin, saponin, quinone, steroid and triterpenoid compounds. Result of inhibitory potential test indicated that treatment D obtained the highest inhibitory potential, while the minimum inhibitory potential was found in treatment A. The best co-culture test result was also found in treatment D, in which 24 hours after co-culture was performed, no V. harveyi colonies (total bacteria of 0 CFU/mL) were found. Bawang hutan bulbs extract in this study was able to inhibit the growth of V. harveyi.