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Articles by issue : Vol 10, No 4 (2016): December 2016
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Articles
The use of Sprout as Precursor for the Production of Indole Acetic Acid by Selected Plant Growth Promoting Rhizobacteria Grown in the Fermentor

ANTONIUS, SARJIYA, BUDISATRIA, RACHEL, DEWI, TIRTA KUMALA

Microbiology Indonesia Vol 10, No 4 (2016): December 2016
Publisher : Indonesian Society for microbiology

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Abstract

Indole-3-acetic acid (IAA) is the main member of the auxin family that controls many important physiological processes in plant. Such beneficial IAA that produced by plant growth promoting rhizobacteria (PGPR), enhances plant growth and was believed to increase the access to more nutrients in the soil. The precursor for syntetizing IAA is tryptophan, and it was also found in the sprout or other sources of protein. The aim of this study was to investigate the best bacteria and growth medium supplemented with extract of bean sprout or fish meal as the sources of precursor for the IAA production. Several bacterial isolates were screened for highest IAA production. IAA production was measured with High Performance Liquid Chromatography. All of isolates were able to produce IAA and isolates PS1 was selected for the further assay by cultivating under fermentor system. Sequencing of 16S rDNA of PS1 isolate indicated as Acinetobacter sp. The result showed that the highest IAA production during fermetation was 62,428 ppm found in under medium supplemented with mung bean sprout extracts grown in fermentor, after 24 hours incubation.

Effect of catfish’ (Clarias gariepinus) flour and oil with probiotic Enterococcus faecium IS-27526 based functional feed provision on bodyweight and C-reactive protein (CRP) of aged atherogenic female Cynomolgus monkey (Macaca fascicularis)

RIFQI, MAHMUD ADITYA, KOESHARTO, CLARA M., SURONO, INGRID S, MARLIYATI, SRI ANNA

Microbiology Indonesia Vol 10, No 4 (2016): December 2016
Publisher : Indonesian Society for microbiology

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Abstract

The aim of the research was to study the effect of functional feed of catfish’ flour, oil and probiotic E. faecium IS-27526 based on bodyweight and CRP (C-reactive protein) of aged female Cynomolgus monkey (Macaca fascicularis). Nine aged female Cynolmolgus randomly divided into three groups. The age is determined by dentition, with bodyweight in a range of  2 – 4 kg. Animals were placed in individual cages in the position where they can interact audiovisually. Feed composition consists of sugar, egg, soy flour, wheat flour, sweet potato flour, butter, egg yolk flour, catfish’ flour and oil, and microencapsulated probiotic Enterococcus faecium IS-27526 and administered for 90 days. Evaluation of bodyweight (BW) and CRP were conducted. There is no significant effect (p>0.05) of experimental diets on bodyweight in each group. However, probiotic tends to delay the bodyweight gain. The bodyweight of cynomolgus in probiotic diet is shown more stable than others. There is no the effect of experimental diets on CRP which is marked by negative result of CRP test. Probiotic E. faecium IS-27526 is potential for bodyweight homeostasis regulation to reduce the risk of overweight and obesity.

Selection and Bioassay of Azotobacter sp. Isolates to Improve Growth of Chili (Capsicum annum L.) on Entisols in Ambon

HINDERSAH, REGINAWANTI, PRIYANKA, PRIYANKA, RUMAHLEWANG, WILHELMINA, KALAY, A MARTHIN

Microbiology Indonesia Vol 10, No 4 (2016): December 2016
Publisher : Indonesian Society for microbiology

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Abstract

Leafy vegetables contributes to the inflation rate in Ambon City due to low productivity in rainy season. Some vegetables are imported from other islands while importantvegetables such as local petsai (Brassica chinensis L.) and chili (Capsicum annum L.) are cultivated in low nitrogen soil, Entisols. Lack of nitrogen could be overcome by using inorganic fertilizeras well as biofertilzer. The soil can be inoculated with rhizobacteria, such as Azotobacter, to increase  the nitrogen uptake and improve the quality of vegetables. This research was conducted to isolate and select Azotobacter from rhizosphere of vegetables and to examine the effect of Azotobacter inoculation on chili-seedling growth and nitrogen uptake by using bioassay method. Azotobacter sp. was isolated in nitrogen-free Ashby’s Media. The bioassay was held in the green house with randomized block design experiment, which examined the combination of isolates and population of Azotobacter sp. on chili. Two best isolates which was selected based on pH, nitrogen content and cell viability were s2a10 (from petsais rhizosphere) and c2a9 (from chili’s rhizosphere). Bioassay showed that Azotobacter inoculation followed by reduced NPK fertilizer doses had no effect on transplant dry weight and nitrogen uptake. All Azotobacter 8 -1inoculation except  10 CFU mL s2a10 maintain soil nitrogen although Azotobacter population in soil was slightly reduced. This showed that Azotobacter sp. potentially reduce the use of inorganic biofertilizer.

Cloning and Expression of Small Hepatitis B Surface Antigen (sHBsAg) In Hansenula polymorpha

HERYAKUSUMA, CHRISTIAN, PUSPASARI, FERNITA, IHSANAWATI, IHSANAWATI, GIRI-RACHMAN, ERNAWATI ARIFIN, TAN, MARSELINA IRASONIA, RAMADHANI, EKAPUTRA, NURAINY, NENI, NATALIA, DESSY

Microbiology Indonesia Vol 10, No 4 (2016): December 2016
Publisher : Indonesian Society for microbiology

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Abstract

Recombinant small hepatitis B surface antigen (sHBsAg) is used as a vaccine component to prevent hepatitis B virus infection. As an attempt to produce local recombinant sHBsAg, a PCR-amplified DNA fragment encoding Indonesia sHBsAg which belongs to B genotype and adw2 subtype was cloned into Hansenula polymorpha expression vector pHIPX4 by using recombination method. The resulted pHIPX4-sHBsAg was integrated into the alcohol oxidase (AOX) locus of H. polymorpha NCYC495 genome and the sHBsAg expression was regulated under the control of H. polymorpha AOX promoter. H. polymorpha NCYC495 carrying the sHBsAg coding sequence was grown in mineral medium and methanol 0.5% (v/v) was added to induce the expression of recombinant sHBsAg. The expression of sHBsAg was detected by HBsAg diagnostic kit test, ELISA, and Western blot analysis.

ITA REGISTRATION FORM AND BACK COVER

Helianti, Is ( 1. BPPT 2. Indonesian Society for Microbiology )

Microbiology Indonesia Vol 10, No 4 (2016): December 2016
Publisher : Indonesian Society for microbiology

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Abstract