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Articles by issue : Vol 10, No 3 (2016): September 2016
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Articles
Application of Response Surface Method in Optimization of Medium Composition for Xylanase Production by Bacillus halodurans CM1 in Submerged Fermentation

WIBOWO, SARA GUSTIA ( 1. BPPT 2. Indonesian Society for Microbiology ) , HELIANTI, IS, SURYANI, ANI, WAHYUNTARI, BUDIASIH

Microbiology Indonesia Vol 10, No 3 (2016): September 2016
Publisher : Indonesian Society for microbiology

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Abstract

A two level factorial design was performed to optimized xylanase production by alkalothermophilic Bacillus halodurans CM1 using response surface method. The variables involved in this experiment were carbon (X), 1 nitrogen source (X) concentration, and pH (XCorn cob and fish powder were use as carbon and nitrogen source 2 3 respectively. Statistical analysis of the experimental results in the range studied, only carbon source gave significant effect on xylanase production.  A second-order model was proposed to represent the enzyme activity as a function of xylan concentration (X) and pH (X).  The optimum corn cobs concentration was 4.37% (w/v), 1 3 fish powder P concentration was 1.75% (w/v) and pH 9. These conditions were tested and validated experimentally since the maximum growth rate achieved with these parameters, and the highest xylanase activity.

Enhancing the Removal of Highly Concentrated CO2 Through Synergism between Microalgae Consortium and Nutrient Ratio in Photobioreactor

RINANTI, ASTRI ( Trisakti University ) , DEWI, KANIA ( Bandung Institute of Technology, Environmental Engineering Department )

Microbiology Indonesia Vol 10, No 3 (2016): September 2016
Publisher : Indonesian Society for microbiology

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Abstract

This research was carried out by developing the carbon capture and storage (CCS) technology to determine the synergism between microalgae consortium and the optimum nutrient ratio as an effort to obtain higher CO2 removal efficiency and CO2 utilization efficiency. The microalgae consortium consisting of Chlorella sp., Scenedesmus sp. and Ankistrodesmus sp. have been selected previously as potential candidates for Microbial Carbon Capture and Storage (MCCS) agent and already cultured continuously in PHM (Provasoli Haematococcus Media) artificial medium, in vertical column photobioreactor. Pure CO2 gas at a high concentration of 10% (v/v) flowed from the bottom of vertical column photobioreactor continuously with optimum flow rate of 5 L.min-1. A growth medium (PHM) containing artificial nutrients was flowed continuously at flow rate 7L.day-1 and detention time 3.8 days. Four fluorescent lamps were positioned outside the photo-bioreactor to obtain light intensity of 4000 lux, set for 16 hours light exposure and 8 hours dark, with operating temperature 30°C maintained during the study. Three compositional variations of microalgae consortium were used. They are as follows;  Ch : Sc : An = 1: 1: 1; Ch : Sc = 1: 1; and Ch : An = 1: 1, where Ch, Sc, and An  were  Chlorella sp., Scenedesmus obliquus and Ankistrodesmus sp., respectively. The following variations of nutrient composition were used; C: N: P = 100: 10: 1, C: N: P = 100: 50: 1 and C: N: P = 100: 25: 1. The C, N, and P sources were CO2 (inorganic), KNO3, and KH2PO4, respectively. This study proves that synergism between the types making up the consortium also determined the ability to utilize inorganic carbon source. Without the presence of Ankistrodesmus sp., synergism between Scenedesmus obliquus and Chlorella sp. showed twice higher CO2 utilization efficiency in comparison to the synergism between Ankistrodesmus sp and Chlorella sp. Increased nitrogen concentration in medium increased the growth of Chlorella sp and Scenedesmus obliquus as a consortium, the CO2 removal efficiency, the CO2 utilization efficiency and the Carbon Uptake Rate. The nutrient ratios C:N:P of 100:50:1 could increase CO2 utilization efficiency upto 50% higher than the C:N:P of 100:10:1.

Screening of Antibiofilm Activity from Marine Bacteria against Pathogenic Bacteria

CAMESI, ALIANDA BUDHIRIANi ROSSATI ( Atma Jaya Catholic University of Indonesia ) , LUKITO, AGUSTINA ( Atma Jaya Catholic University of Indonesia ) , WATURANGI, DIANA ELIZABETH ( Atma Jaya Catholic University of Indonesia ) , KWAN, HWANG JAE ( Yonsei University )

Microbiology Indonesia Vol 10, No 3 (2016): September 2016
Publisher : Indonesian Society for microbiology

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Abstract

Bacterial biofilms produced by pathogenic bacteria have become a serious issue in several chronic diseases such as atherosclerosis, cystic fibrosis, endocarditis, inner ear infections, and kidney stones. Thus, inhibition and destruction of bacterial biofilm from pathogenic bacteria is needed. The purpose of this study is to analyze biofilm inhibition and destruction activities of marine bacteria associated with hard and soft corals isolated from several oceanic regions in Indonesia. Fifteen marine isolates collected from several regions in Indonesia such as Bali Province, South East Sulawesi Province, East Java Province, Lampung Province, and Banten Province were tested using static biofilm assay against several pathogenic bacteria. Biofilm of the pathogenic bacteria tested were stained using 0.4% crystal violet. Several isolates were sequenced using 16S rRNA PCR method. Most of marine isolates presented higher inhibition and or destruction activity at 10% crude concentration. Few isolates were further identified using 16S rRNA and proven to have antibiofilm activity against several pathogenic bacteria. Marine bacteria have broad applications in medical and pharmaceutical industries and the oceanic regions of Indonesia are promising sources for the discovery of novel bacteria with antibiofilm activity.

Cloning and Heterologous Expression of Extracellular Plantaricin F Produced by Lactobacillus plantarum S34 Isolated from “Bekasam” in Lactococcus lactis

MUSTOPA, APON ZAENAL, MURTIYANINGSIH, HIDAYAH ( science center for biotechnology LIPI ) , FATIMAH, FATIMAH, SUHARSONO, SUHARSONO

Microbiology Indonesia Vol 10, No 3 (2016): September 2016
Publisher : Indonesian Society for microbiology

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Abstract

Plantaricin F (pln F) is bacteriocins produced by Lactobacillus plantarum are mostly applied in food to prevent microbial contamination. Biosynthesis of pln F is controlled by plantaricin A (pln A) which is primarily a peptide pheromone that controls the production of antimicrobial peptides in L. plantarum. Pre-mature pln A contains signal peptide and utilizes the general secretory pathway for export this peptide. The aim of this study was to construct a fusion of pln A signal peptide with mature pln F and to investigate the antimicrobial activity of pln F. Extracellular pln A- encoding the plnA gene were cloned into pGEM-Teasy vector to be used as a source for signal peptide SPplnA. A polymerase chain reaction (PCR) overlaps technique has been used in the construction of the fused gene with size of 171 bp while the individual gene obtained by this technique was 66 bp for pln A signal peptide and 105 bp for pln F. A gene encoding the pln A signal peptide (SPplnA) fused to mature plantaricin F,  fused gene were then cloned into pNZ8148 as expression vector under the control of the nisin promoter (Pnis A) to generate a pNZ8148 SPplnA-plnF. Molecular expression study showed that recombinant Lactococcus lactis NZ3900 was able to express the mature pln F at transcription and translation level with size of 171 bp (by RT-PCR) and 3.8 kDa (by SDS-PAGE), respectively after 0.5-5 ng/ml nisin induction (OD600 0,5). Furthermore, the supernatants of the recombinant L. lactis NZ3900 showed antimicrobial activity against Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6539 and Listeria monocytogenes BTCC B693. Collectively, the successfulness of expression of functional pln F gene under the control of nisin induction in L. lactis NZ3900, for the first time.