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Articles by issue : Vol 1, No 1 (2018): March 2018
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Articles
Physiological Profiling and Microorganism Community Analysis of Cirebon Shrimp Paste Fermentation “Terasi” using BIOLOGTM EcoPlate

ASTUTI, DEA INDRIANI, TAUFIK, INTAN, ACHNAFANI, DINI, PRISCILA, EZRA SUCI

Microbiology Indonesia Vol 1, No 1 (2018): March 2018
Publisher : Indonesian Society for microbiology

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Abstract

Terasi or shrimp paste is an Indonesian traditional seasoning made from fermented small shrimp or krill. Different indigenous microorganism community exhibit different physiological function due to lack standard in its materials and processing. This study aimed to determine physiological profiles and microorganism community in Cirebon shrimp paste fermentation. BIOLOGTM EcoPlate was used to obtain microbial physiological function of the krill and 2-months old shrimp paste. Microorganisms were later isolated from EcoPlate substrate to determine its community structure. Average Well Color Development (AWCD) from krill was thirty times higher than shrimp paste. Interestingly, this study revealed a shift of carbon source utilization at day-28 of fermentation from amino acid and polymer to phenolic compound. In addition, AWCD index increased in accordance with increased of microorganism community complexity at day-28. Within 56 days of fermentation there was a slightly increase in water, fat, and carbohydrate content. In contrast, there was decrease in protein, ash content, and acidity level from neutral to acid, with salinity level resulted in between 16.26% to 21.42%. We conclude that there is a change of microorganism community within shrimp paste fermentation corresponding to metabolism activity which affects the product quality. 

THE EFFECT OF CARBON AND NITROGEN SUPPLEMENTATION ON BACTERIOCIN PRODUCTION OF LACTIC ACID BACTERIA FROM PICKLED YELLOW BAMBOO SHOOTS (Dendrocalamus asper)

HARTAJANIE, LAKSMI ( Fakultas Teknologi Pertanian UNIKA Soegijapranata Semarang ) , LINDAYANI, LINDAYANI ( Fakultas Teknologi Pertanian UNIKA Soegijapranata Semarang ) , SANTOSO, LORENTIA ( Fakultas Teknologi Pertanian UNIKA Soegijapranata Semarang )

Microbiology Indonesia Vol 1, No 1 (2018): March 2018
Publisher : Indonesian Society for microbiology

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Abstract

Six selected lactic acid bacteria (LAB) isolates from pickled Yellow Betung bamboo shoots were grown in de Mann Rogosa Sharpe-Broth (MRSB) media with different supplementation combination. The cell-free supernatant were evaluated for their ability to produce bacteriocin  by adjusting its pH to 6.0 in order to remove organic acid effects. The bacteriocin activity was  assayed by agar-well diffusion method. The inhibitory activity calculated in Activity Unit (AU in mm2 mL-1) of bacteriocins. The aims of this paper is to explore the effect of different medium compositions on bacteriocin production and its inhibitory activity against pathogenic bacteria (Listeria monocytogenes FNCC 0156, Staphylococcus aureus FNCC 0047, and Escherichia coli FNCC 0091).Supplementations of carbon and nitrogen sources induced production of bacteriocins. LAB isolates grown in media without supplementation could not produce bacteriocins.  Growth of isolate D44 in the presence of 2% of glucose and 2% of yeast extract yielded the largest bacteriocin inhibitory activity levels of 3179 AU mL-1 against Listeria monocytogenes FNCC 0156, 4663 AU mL-1 against Staphylococcus aureus FNCC 0047, and 3109 AU mL-1 against Escherichia coli FNCC 0091.

Bacterial leaching of an Indonesian complex copper sulfide ore using an iron-oxidizing indigenous bacterium

CHAERUN, SITI KHODIJAH, PUTRI, FRIDENI YUSHANDIANA, MINWAL, WAHYUDIN PRAWITA, ICHLAS, ZELA TANLEGA, MUBAROK, MOHAMMAD ZAKI

Microbiology Indonesia Vol 1, No 1 (2018): March 2018
Publisher : Indonesian Society for microbiology

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Abstract

The bioleaching of an Indonesian complex copper sulfide ore was studied in shake flasks over a period of 14 days using an iron-oxidizing indigenous bacterium at room temperature (28 oC) and various pulp densities (5% and 20%). The bioleaching suspensions were periodically analyzed for Cu and Fe concentrations as well as Eh, pH and DO values. Cu bioleaching efficiencies at 5% pulp density were higher than those at 20% pulp density, which correlated with Fe concentration in solution. Over a period of 14 days, the pH of bioleaching suspension was in the range of 5 ~ 9, indicating that Cu bioleaching was greatly influenced not only by proton H+ dan ferric ion but also by extracellular polymeric substances (EPS) generated by the bacterium. The current study may improve our better understanding on the bacterial action for bioleaching of complex copper sulfide ores that remains debated so far as refractory ores.