cover
Filter by Year
BIOMA
Published by Universitas Diponegoro
BIOMA (ISSN 1410-8801) adalah Majalah Ilmiah Biologi yang bertujuan mewadahi semua informasi hasil penelitian, telaah pustaka, makalah teknis, dan kajian buku, dari berbagai cabang ilmu Biologi. Diharapkan penerbitan ini dapat berkontribusi dalam penyampaian informasi ilmiah yang merupakan mata rantai proses kegiatan ilmiah. Terbit sejak tahun 1999.
Articles by issue : Vol. 15, No.2, Tahun 2013
7
Articles
Aplikasi Pakan Kaya Karotenoid Hasil Fusi ProtoplasmIntergenera Dunaliella salina dan Chlorella vulgaris pada Udang Windu (Penaeus monodon F.) Stadia PL-20 Di Desa Asempapan, Pati, Jawa Tengah

Rahmawati, Novia, Zainuri, Muhammad, Kusumaningrum, Hermin Pancasakti

Bioma Vol. 15, No.2, Tahun 2013
Publisher : Bioma

Show Abstract | Original Source | Check in Google Scholar | Full PDF (799.549 KB)

Abstract

Dunaliella salina and Chlorella vulgaris is a natural feed microalgae with high carotenoid content that can be increased using protoplast fusion technique. Protoplast fusion as one of the application fields of genetic engineering is a method for obtaining recombinant with the desired properties and profitable in a short time. This study aimed to see the effect of the addition of carotenoid-rich feed results from protoplast fusion recombinant D. salina and C. vulgaris on the survival rate and weight of shrimp post larvae. Mixed fusion results feed and artificial feed needed for the growth of post-larval shrimp, moulting and skin pigmentation. The results showed that the recombinant from protoplast fusion intergenera D. salina and C. vulgaris contains carotenoid pigments higher, reaching 124.6 mg / g bks from the second parent, namely D. salina reached 101.83 mg / g bks, while C. vulgaris 97.18 ug / g bks. Feed manufacturing is done by mixing pellets and 80-100x103 cells per 0.0225 g of feed. Application of feed carried on Penaeus monodon F. (tiger prawn) stadia PL-20 for a month. The results of weight measurements showed the highest prawn post larvae reached at artificial feeding plus recombinant protoplast fusion results intergenera D. salina and C. vulgaris and was able to raise the level of post-larval shrimp survival rate reached 88%.   Keywords: D. salina, C. vulgaris, Protoplast Fusion, Carotenoid, Penaeus monodon F.

Kinetika Pertumbuhan Dan Produksi Inulinase Fusan F7

-, Wijanarka, Soetarto, Endang Sutariningsih, Dewi, Kumala, Indrianto, Ari

Bioma Vol. 15, No.2, Tahun 2013
Publisher : Bioma

Show Abstract | Original Source | Check in Google Scholar | Full PDF (74.963 KB)

Abstract

Pertumbuhan dapat diartikan sebagai suatu pertambahan bagian-bagian sel. Adanya     pertumbuhan sel biasanya dapat diketahui dengan adanya pertambahan ukuran dan     pembelahan sel. Populasi sel khususnya mikroba  secara kuantitatif atau kualitatif dapat digunakan untuk memantau atau mengkaji fenomena pertumbuhan. Enzim inulinase (E.C. 3.2.1.7) adalah enzim yang mampu merombak substrat inulin menjadi monomer fruktosa. Fruktosa merupakan bahan baku (doctoring agent) untuk proses  pembuatan FOS, IOS, pulullan, aseton dan sorbitol. Tujuan  penelitian ini adalah untuk mengetahui kinetika kecepatan pertumbuhan specifik (µ), waktu generasi (g) dan aktivitas inulinase yang dihasilkan oleh fusan F7.  Fusan F7 merupakan hasil fusi antara Pichia manshurica dan Rhodosporidium paludigenum. Hasil penelitian menunjukkan bahwa Fusan F7 mempunyai kecepatan pertumbuhan specific (µ)  sebesar 0.3299 jam dengan waktu generasi (g) 2.1012 jam dan aktivitas enzim inulinase yang dihasilkan sebesar  0.5337 IU. Hasil tersebut  terletak diantara kedua parentalnya yaitu P. manshurica (µ= 0.27935 jam; g = 2.4815 jam dan aktivitas = 0.557 IU) dan Rh. paludigenum (µ= 0.3787 jam; g = 1.8304 jam dan aktivitas = 0.3263 IU).   Kata kunci : Pertumbuhan;  fusan F7; inulinase ; umbi dahlia

Keragaman Jenis dan Kelimpahan Populasi Penggerek Batang Padi dan Serangga Lain Berpotensi Hama Pada Ekosistem Sawah Organik

Hadi, Mochamad, Soesilohadi, RC Hidayat, Wagiman, FX, Soehardjono, Yayuk Rahayuningsih

Bioma Vol. 15, No.2, Tahun 2013
Publisher : Bioma

Show Abstract | Original Source | Check in Google Scholar | Full PDF (80.643 KB)

Abstract

Rice stem borer is a main pest of paddy rice in Central Java. The main rice stem borer are yellow rice borer, Scirpophaga incertulas Walker, and white rice borer, S. innonata Walker. Other species are pink borer Sesamia inferens Walker, striped rice borer Chilo suppressalis Walker, dark-headed rice borer, C. polychrysus Meyrick, and glossy rice borer, C. auricilius Dudgeon. Besides rice stem borer, there are many other insect which are potential as rice pest, i.e., grasshopper, rice ear bug, brown planthopper, etc. Organic agriculture in Central Java have not been applied yet, hence there is still lack of information, including species diversity information and abundance of rice stem borer population and other potential insect pest on rice. Species diversity and population abundance of rice stem borer and other potential insect in organic rice ecosystem differ with the inorganic one. The objective of this research is to study species diversity and abundance of rice stem borer population and other potential pest in organic rice ecosystem. The study was conducted in organic rice of Bakalrejo Village, Susukan Sub district, District of Semarang. Species diversity and population abundance was analyzed using Shanon-Wiener’s species diversity index (H’), population abundance index (Di), and population evenness index (e). Pattern of  species diversity, population abundance, population evenness were temporally analyzed using population dynamic curve. Sampling of species diversity and population abundance was performed during a crop planting season with twice of sampling frequency. The result shows that rice stem borer found  were yellow rice borer with its population fluctuated temporally and pink rice borer with  its population were few and scarce. The highest diversity found during rice generative phase until ripen and decline in line with the age of rice. Species diversity index in vegetative phase was below 2, while during generative phase until ripen increased above 2. Insect population abundance declined in the end vegetative phase and increased in the beginning of reproductive phase until ripen phase. Population evenness index in all growth phase of rice were relatively high and similar. Keywords : organic rice, rice stem borer, insect pest of rice.

Penambahan Filtrat Tepung Umbi Dahlia (Dahlia variabilis Willd.) sebagai Prebiotik dalam Pembuatan Yoghurt Sinbiotik

Alfaridhi, Khabib Khasan, Lunggani, Arina Tri, Kusdiyantini, Endang

Bioma Vol. 15, No.2, Tahun 2013
Publisher : Bioma

Show Abstract | Original Source | Check in Google Scholar | Full PDF (548.421 KB)

Abstract

Yoghurt is a fermented milk product that can provide beneficial effects to the health of consumers. Increasing the quality of yoghurt can be made by combining the benefits of probiotic with prebiotic to produce sinbiotic yoghurt. Inulin is a prebiotic that found in many dahlia tuber (Dahlia variabilis Willd.). Pure inulin were expensive, so the alternative is using dahlia tuber flours filtrate as prebiotic ingredients. This study aimed to examine the addition of Dahlia Tuber Flours Filtrate (DTFF) as a prebiotic in sinbiotic yoghurt making using Lactic Acid Bacteria (LAB) Lactobacillus bulgaricus, Streptococcus termophilus dan Lactobacillus acidopilus as probiotic. This research using Completely Randomized Design (CRD) with 4 concentration treatments DTFF 0%, 3%, 6% and 9% with 3 replications. Observed variables consist of the number of total LAB, lactic acid level, pH and organoleptic attributes (aroma, flavor, consistency and impression). Data the number of total LAB, lactic acid level and pH values ​​were analyzed by ANOVA, continued by Duncan test, while the organoleptic data was analyzed by Mann-Whitney U test with 95% significant level. The results showed that the addition of DTFF significantly affect the increasing of total LAB, lactic acid level, organoleptic assessment and decreasing pH of sinbiotic yoghurt. Sinbiotic yoghurt with 3% of DTFF concentration is most preferred by panelists and required the criteria of Indonesian National Standard (SNI) for yoghurt with enough likely aroma, sour taste/specify and enough thick texture with total LAB 2,4 x 108 CFU/ml, 1.3% lactic acid levels and 4.2. pH. Keywords : prebiotic, probiotic, inulin, dahlia tuber, sinbiotic yoghurt, lactobacillus bulgaricus, streptococcus termophilus, lactobacillus acidopilus

Prediksi Resistensi Udang Vaname (Litopenaus vannamei) terhadap Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) dari Tambak Intensif dan Semi Intensif Jepara Menggunakan Marka RAPD

Mulyadi, Muhammad, Handayani, Christina Ratna, Kusumaningrum, Hermin Pancasakti, Budiharjo, Anto

Bioma Vol. 15, No.2, Tahun 2013
Publisher : Bioma

Show Abstract | Original Source | Check in Google Scholar | Full PDF (91.931 KB)

Abstract

Infectious Hypodermal and Hematopoietic Necrosis Infectious Virus (IHHNV) is the most important DNA virus which can lead to Runt Deformity Syndrome (RDS) in vaname shrimp. The RAPD technique can be used to determine the resistance of a species to a disease. This research aimed to screen and identify RAPD markers which could distinguish the resistance of vaname shrimp to IHHNV reared at intensive and semi-intensive pond. The DNA template was amplified by PCR using 5 primers : OPA 06, OPA 08, OPA 19, OPD-02 and OPZ-15. The results showed that only the primer OPA-19 and OPZ-15 were able to produce 100% polymorphic bands with sizes from 400-1700 bp as well as showing the resistance IHHNV in vaname shrimp. Based on these results, vaname shrimp which reared at the intensive pond were more resistant to IHHNV compared with the semi-intensive pond.   Key words: RAPD, shrimp vaname, IHHNV, intensive & semi-intensive pond  

Struktur Komunitas Mesofauna Tanah dan Kapasitas Infiltrasi Air setelah diberi Perlakuan Biostarter Pengurai Bahan Organik

Andriani, Latifah Fitria, Rahadian, Rully, Hadi, Mochamad

Bioma Vol. 15, No.2, Tahun 2013
Publisher : Bioma

Show Abstract | Original Source | Check in Google Scholar | Full PDF (364.085 KB)

Abstract

Research about the biostarter application on the biopori concept through parameter of soil mesofauna community structure and water infiltration capacity was aimed to investigate the effect of biostarter product on soil mesofauna community structure and water infiltration rate at Lubang Resapan Biopori (LRB) aged 30 days and 50 days, also the correlation between mesofauna community structure and soil infiltration capacity. This study uses a single factor completely randomized design, which has 5 treatments including control, EM4, Bioklin, Mig Dec, and Orgadec. The results showed that the addition of biostarter affects soil mesofauna community structure, particularly positive effect on the abundance of Oribatida and Mesostigmata. Products Orgadec was able to increase faster the abundance of mesofauna both at the age of LRB 30 days and 50 days, whereas Mig Dec and EM4 starting to show its influence on the LRB after 50 days. Infiltration rate LRB age of 30 days showed a significant difference in treatment of Orgadec only, whereas at the age of 50 days, the significant difference is indicated in the treatment EM4​​, Mig Dec and Orgadec. Based on the Spearman correlation test, the total number of individuals and number of Oribatida. Both showed significant positive correlation with the infiltration rate.   Keywords: biostarter, community structure, soil mesofauna, infiltration rate, LR

Perbedaan Kualitas Lahan Apel Sistem Pertanian Intensif dengan Sistem Pertanian Ramah Lingkungan (Studi Kasus Di Kelompok Tani Makmur Abadi Desa Tulungrejo Kecamatan Bumiaji Kota Batu)

Indahwati, Retno, Hendrarto, Ign Budi, Izzati, Munifatul

Bioma Vol. 15, No.2, Tahun 2013
Publisher : Bioma

Show Abstract | Original Source | Check in Google Scholar | Full PDF (82.467 KB)

Abstract

The aim of this research to analyze the difference of apple farm quality the influence of  intensification of agriculture system with environmentally-friendly of agriculture system. A descriptive field research was conducted in June until August 2012. The soil samples were taken in each location by using disturb sample method. The Arthropods sample were taken every five days for five time by using pitfall trap method with five transek in each location.The research applied a  qualitative and quantitative-descriptive. The farm quality were comparison soil quality analyze and  compositions of ground Arthropods. The composition of ground Arthropods were analyzed using Important Value Index and the diversities were analyzed by using Shannon Wiener Index. The result showed that farm quality with environmentally-friendly of agriculture system was better than intensification of agriculture system. The ground Arthropods collected at 150 pitfall trap in environmentally-friendly of agriculture system were 15.079 individual while those in intensification of agriculture system were 9.461 individual. Based on Important Value Index (40,83-64,31), Collembolla ordo Entomobryidae family dominated in each location. Based on Shannon Wiener Index that diversity of ground Arthropods in intensification of agriculture system (H’= 1,58-2,04) was greater than environmentally-friendly of agriculture system (H’= 1,56- 1,99), but both of them at medium criteria.   Keywords : farm quality, agriculture system, Arthropods composition