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BIOMA
Published by Universitas Diponegoro
BIOMA (ISSN 1410-8801) adalah Majalah Ilmiah Biologi yang bertujuan mewadahi semua informasi hasil penelitian, telaah pustaka, makalah teknis, dan kajian buku, dari berbagai cabang ilmu Biologi. Diharapkan penerbitan ini dapat berkontribusi dalam penyampaian informasi ilmiah yang merupakan mata rantai proses kegiatan ilmiah. Terbit sejak tahun 1999.
Articles by issue : Vol. 15, No.1, Tahun 2013
7
Articles
Analisis Vegetasi Pada Habitat Gajah Sumatera Di Suaka Margasatwa Padang Sugihan Provinsi Sumatera Selatan

Mahanani, Agnes Indra, Hendrarto, Ign Budi, Soeprobowati, Tri Retnaningsih

Bioma Vol. 15, No.1, Tahun 2013
Publisher : Bioma

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Abstract

Padang Sugihan Wildlife Reserve covering 86,932 hectares is the natural habitat of elephants in South Sumatra, both wild and managed elephants at Elephant Training Center, Sub Padang Sugihan. This area was used to be production forest. To support life of wild elephants, the vegetation quantity and diversity of vegetation types of feed must be known. This study aimed to analyze the vegetation feed elephants in Padang Sugihan Wildlife Reserve. The method used sampling plots with a size of 1m x 1m and 5m x 5m in five study sites. Data were analyzed using analysis of vegetation to get the importance value index. Species diversity was calculated using the Shannon Wienner Index formula. It was also calculated the value of uniformity of plant based on Simpson formula. The results of importance value index showed that location I was dominated by Echinochloa colonum (L.) Link (76.55%), location II Panicum astagninum R (132.48%), location III  Panicum repens L (107.14%), location IV Axonopus caompressus ( 79.48%), location of V Panicum repens L and Cynodon dactylon (L.) Pers. (21.28%), location VI Melaleuca cajuputi (80.77%), and location VII Melaleuca cajuputi (84.08%). The value of the highest species diversity in the location of V at 2.53. The lowest value was in location II at 0.66. The highest uniformity index was 0.92. While the lowest value was 0.37 in the location II. Based on the above results it is necessary to enrich the vegetation species of elephant grass and natural food, especially at locations II and III.   Keyword: Padang Sugihan Wildlife Reserve, vegetation analysis, Elephas maximus sumateranus Temminck  

Komposisi, Kemelimpahan dan Keanekaragaman Fitoplankton Danau Rawa Pening Kabupaten Semarang

Samudra, Sesilia Rani, Soeprobowati, Tri Retnaningsih, Izzati, Munifatul

Bioma Vol. 15, No.1, Tahun 2013
Publisher : Bioma

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Abstract

Rawa Pening is a semi natural lake which is utilized for hydro-electric power plant,  caged fish culture, irrigation, and tourism. It belongs to one of the fifteen lakes which receives national priority to be saved and preserved because of its very poor condition as a result of eutrophication, sedimentation and degraded water quality. Eutrophication of  Rawa  Pening comes from the Water Catchment Area,  originating from farms, animal husbandry, domestic and industrial waste around the lake, and also from the water body itself, that is from caged fish culture. The fertility criteria of the lake water can be determined on the basis of the abundance and variety of phytoplankton and the total phosphorus content. The aim of this research is to find out the water fertility criteria of Lake Rawa Pening based on the abundance and variety of  phytoplankton, and the phosphorus content. Research began in July 2012 on three stations. Station I is an area with fishcage culture;  Station II is an area without fishcage culture, and Station III is the river inlet or water catchment area. Each station consisted of three different sampling areas. The phytoplankton abundance at the station without  fishcage culture is higher  (19012 ind/l) than at the fishcage culture station (14356 ind/l)  as well as at the inlet station (11058 ind/l), but the diversity index at the no fishcage station is lowest (1.80)  compared to the fishcage culture station (2.32)  and the inlet station (2.05). The fertility criteria of Rawa Pening based on the phytoplankton abundance and P-total  of  its water is eutrophic  going  towards  hypereutrophic.   Keywords : Lake Rawa Pening, Phosphorus, Water Quality, Euthropication

Optimasi Isolasi DNA Cabai (Capsicum annuum L.) Berdasar Perbedaan Kualitas dan Kuantitas Daun serta Teknik Penggerusan

Ferniah, Rejeki Siti, Pujiyanto, Sri

Bioma Vol. 15, No.1, Tahun 2013
Publisher : Bioma

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Abstract

Complete genome of chili has not been reported.The first step to study the genome is DNA isolation, so it is necessary to optimize the protocol to get an optimum DNA. This research aimed to optimize chili DNA isolation by variate the quantity and quality of chili leaves as row material and variate the grinding technique. DNA isolation was done using commercial kit without liquid nitrogen, and analyzed by agarose gel electrophoresis. The results showed that frozen chili leaf yields more DNA than fresh leaf, 0,1 g of leaf got optimum DNA, and grinding in mortar was better than in microtube.   Key words: DNA,  isolation, Capsicum annuum

Pelacakan Gen Sitokrom Oksidase Sub Unit I (COI) DNA Mitokondria Itik Tegal (Anas domesticus) Menggunakan Primer Universal

Wibowo, Sarwo Edi, Djaelani, Muhammad Anwar, Kusumaningrum, Hermin Pancasakti

Bioma Vol. 15, No.1, Tahun 2013
Publisher : Bioma

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Abstract

Currently local ducks are generally quite difficult to find in a big farm in Inonesia, including Tegal ducks. Tegal ducks is one of the genetic resources native to Indonesia with it’s advantages in terms of high and large egg production. Conservation and development of local ducks have strived to maintain our existence of Indonesian livestock germplasm. If such information is not superior to native species exist, the opportunity to increase his lead further also getting smaller. Tracking the mitochondrial COI gene DNA of Tegal ducks may underlie the process of an organisms genetic characterization. Information about Tegal duck mitochondrial DNA has not been done. The information obtained can be used for optimization of duck products native to Indonesia both in physiological aspects, phylogeny and genetic engineering. The research method used in this research is tracking COI gene data from Gen Bank with the programs Clustal X and Genedoc. Tracking then continued using universal primers HCO and LCO. The results of the data followed up with the isolation and amplification of COI gene mitochondrial DNA as well as the optimization of PCR conditions. The results showed mitochondrial DNA COI gene Tegal ducks were amplified with primer LCO obtain DNA fragments of length less than 250 bp. Kata kunci: duck’s from Tegal, COI gene, mitochondrial DNA

Studi Etnobotani Masyarakat Desa Sukolilo Kawasan Pegunungan Kendeng Pati Jawa Tengah

Irsyad, Muhammad Nur, -, Jumari, -, Murningsih

Bioma Vol. 15, No.1, Tahun 2013
Publisher : Bioma

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Abstract

This research aims to reveal the types of plants used by communities for subsistence daily. This research conducted in the village of Kendeng Mountains region Sukolilo, Pati. Data collection conducted exploratory used a roaming method to inventory plant species. This method supported by ethnobotany participatory appraisal techniques consisting of: open-ended interview and participatory observation by the public as key informants. Inventory and interviews made ​​in plant utilization category. Research showed that Sukolilo villagers still had a good knowledge about the diversity of plants and about plants their use in daily life. Inventory data showed that 208 species of plants used by the community, are grouped in categories: food (90 species), medicinal and traditional medicine (44 species), building materials (29 species), fuel wood (13 species), animal feed (11 species), craft materials and tools (8 species), fiber materials and rigging (3 species), and toxic materials (2 species).   Keywords: ethnobotany, cultural value, karst areas, Kendeng mountains

Struktur Komunitas Mikroartropoda Tanah Di Hutan Wisata Gonoharjo Kecamatan Limbangan Kabupaten Kendal

Wahyu Nugroho, Nindta Putra, Rahadian, Rully, Hadi, Mochamad

Bioma Vol. 15, No.1, Tahun 2013
Publisher : Bioma

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Abstract

The study on community structure of soil microarthropod in Gonoharjo Tourism Forest has been done. The objective of this research are to compare community structure of soil microartropod and to know the effect of abiotic environmental factors on abundance of soil microarthropod in Tourism Forest of Gonoharjo. This research was conducted using transect method in four sampling locations i.e., coffe vegetation, pine vegetation, mixed vegetation in hot spring water area, and mixed vegetation of riverside area. Soil sampling were used soil corer, and then the sample were extracted using barlese-tullgren. The finding shows 4 Sub Orders and 22 Families from 10 Orders. Acari was the most dominant group in each observed locations. The most diverse was found in mixed vegetation on hot spring water area, while the least one was found in coffe vegetation. Then, the highest evenness index was found in mixed vegetation on riverside area, while the lowest evenness was found in coffe vegetation. Moreover , the richest soil microarthropods found in mixed vegetation on hot spring water area and the lowest richness was found in mixed vegetation on riverside area. Statistically, abiotic environmental factors that have big impact on soil microarthropod abundance were soil organic matter, soil pH, and soil moisture. .   Keywords : community structure, soil microarthropod, Acari, Tourism Forest of Gonoharjo

Uji Kemampuan Produksi Fruktooligosakarida (FOS) dari Kelompok Aspergillus niger DUCC

-, Whinarsih, Lunggani, Arina Tri, Rukmi, MG Isworo

Bioma Vol. 15, No.1, Tahun 2013
Publisher : Bioma

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Abstract

Prebiotic is a food supplement that can not be digested in the human’s gut, it can stimulate the growth of beneficial microorganisms in the intestine and improve human’s health. FOS is a fructan type of oligosaccharide which is have a potential as a natural prebiotic, it  can be produced by several microorganisms, including A. niger group. The aim of this research was to examined the FOS production of   three isolates of A. niger group from DUCC collection (i.e. DUCC F123, DUCC F129 and DUCC F102). FOS production was determined by measuring the reducing sugar using  DNS method. The result showed that all isolates have the capability in producing  FOS suspected kestose with the degree of polymerization 3.545; 3.215; 3.049 respectively.   Keywords:FOS, fructosyltransferase, Aspergillus niger, prebiotic