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BIOMA
Published by Universitas Diponegoro
BIOMA (ISSN 1410-8801) adalah Majalah Ilmiah Biologi yang bertujuan mewadahi semua informasi hasil penelitian, telaah pustaka, makalah teknis, dan kajian buku, dari berbagai cabang ilmu Biologi. Diharapkan penerbitan ini dapat berkontribusi dalam penyampaian informasi ilmiah yang merupakan mata rantai proses kegiatan ilmiah. Terbit sejak tahun 1999.
Articles by issue : Vol. 13, No. 1, Tahun 2011
7
Articles
Induksi Kalus Binahong (Basella rubra L.) Secara In Vitro Pada Media Murashige & Skoog Dengan Konsentrasi Sukrosa Yang Berbeda

Sitorus, Ertina Novaria, Hastuti, Endah Dwi, Setiari, Nintya -

Bioma Vol. 13, No. 1, Tahun 2011
Publisher : Bioma

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Abstract

Binahong (Basella rubra L.) is a plant medicine consisting secondary metabolites which have virtue asmedicines for several diseases that could also be used as coloring agent. The medicine compounds in secondarymetabolites could be extracted from callus. Sucrose is one of the components that build MS (Murashige & Skoog)medium. Sucrose is important in in vitro culture, it functions as carbon and energy source for explant to grow. Thepurposes of this research are to study the effect of sucrose in MS medium towards B. rubra L callus formation andgrowth; to find the optimum sucrose concentration for callus B. rubra L formation; and also to find the fastestinitiation time to produce callus crumb. This research uses Complete Random Design (CRD) single factor method,i.e., sucrose concentration of 0 g/l, 10g/l, 20 g/l, 30 g/l, 40 g/l with five repetitions. The data is analysed withAnalysis of Varian (Anova) and if a real difference is found the analysis is continued with Duncan Multiple RangeTest (DMRT) with significancy level of 95%. The results show that various sucrose concentrations in MS mediuminfluences callus B. rubra L induction. The highest sucrose concentration, i.e. 40 g/l, which was added into MSmedium, could induce the maximum callus wet-weight of 1,69 g and the fastest callus initiation time of 4,8 day.

Isolasi Yeast Inulinolitik dan Optimasi Produksi Inulinase Pada Berbagai Konsentrasi Nitrogen Yeast Ekstrak Sebagai Sumber N

-, Wijanarka -, Sutariningsih, Endang -, Dewi, Kumala -, Indrianto, Ari -

Bioma Vol. 13, No. 1, Tahun 2011
Publisher : Bioma

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Abstract

Inulin is a linier fructose polymer of plant origin found in the Jerusalem artichoke, dandelion, dahlia tuberand several other members of the family Compositae. Inulin is one of the numerous polysaccharides of plant originthat contain glukose or fructose and that can be used in the food industry and in industrial fermentations as asubtrate. Fifteen yeast growing on inulin as the sole carbon and energi source. An inulinase activity in the liquidculture was measured with sugar reduction. The best optimization conditinos at concentration of 0.75% inulin and 12hour incubation time. On condition that the optimization of inulin activity produced 0.8772 IU by isolate P 12.These yeast have potential uses in the preparation of ingredient food prebiotic.

Jenis–Jenis Tumbuhan Anggota Famili Asteraceae di Wana Wisata Nglimut Gonoharjo Kabupaten Kendal Jawa Tengah

Kumolo, Fredian Bintar, Utami, Sri -

Bioma Vol. 13, No. 1, Tahun 2011
Publisher : Bioma

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Abstract

Famili Asteraceae adalah salah satu jenis famili tumbuhan yang menjadi penyusun vegetasi penutup lantaihutan di Wana Wisata Nglimut. Asteraceae merupakan salah satu famili dengan keanekaragaman jenis yang cukuptinggi dan mempunyai banyak manfaat. Tujuan penelitian ini adalah untuk mengetahui jenis anggota familiAsteraceae yang terdapat di kawasan wana wisata Nglimut Gonoharjo kabupaten Kendal. Hasil penelitian inidiharapkan bisa memberikan informasi dasar dalam pemanfaatan dan konservasinya. Metode penelitian dalampenentuan titik sampling menggunakan tehnik sistematik. Garis transek yang diambil adalah jalur wisata yaitudimulai dari pintu masuk sampai tempat pemandian air panas. Ditentukan 6 titik sampling yang masing-masingberjarak 100 meter sepanjang garis transek. Pada setiap titik sampling dicatat jenisnya dan dihitung jumlahindividunya. Dilakukan pengukuran faktor lingkungan berupa ketinggian tempat dan kelembaban udara. Datadiperoleh dianalisis secara deskriptif.Hasil penelitian diperoleh 6 jenis tumbuhan famili Asteraceae yang tumbuh di wana wisata Nglimut yaituAgeratum conyzoides, Ageratum houstoniamum, Erechtites valerianifolia, Eupatorium riparium, Eupatoriumodoratum, dan Tegetes erecta. Jenis dengan jumlah individu paling banyak dan distribusinya tinggi di WanawisaNglimut adalah Eupatorium odoratum dan Eupatorium riparium, sedangkan jenis tumbuhan famili Asteraceae yangpaling jarang adalah Tegetes erecta.

Lemak Abdominal Ayam Broiler (Gallus sp.) Karena Pengaruh Ekstrak Kunyit (Curcuma domestica Vahl.)

Pratikno, Herry -

Bioma Vol. 13, No. 1, Tahun 2011
Publisher : Bioma

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Abstract

This research is aimes to know the effect of turmeric extract on broiler abdominal fat. Fourty eight broilerchickens strain CP 707 placed on 48 batteray cages and was randomized, then were acclimated during 1 week.Those chicken was alloted into 4 groups, each group was treated as follows : T0 (control) : were not given turmerinextract; T1 : were given turmerin extract 200 mg/kg BW/day; T2 : were given turmerin extract 400 mg/kg BW/day;T3 : were given turmerin extract 600 mg/kg BW/day. Turmerin extract was given on capsul shape. Replication of thetreatment was 6 times. Long of the treatment was 2 steps, step I (L1) turmerin extract was given during 2-3 weeks (8– 21 days age), step II (L2) the treatment was continued during 3 weeks (22 – 42 days age), turmerin extract dosewas ajusted to the chicken body weight. The chicken were feed with BR I and BR II. Food and drinking water weregiven in an ad libitum manner. Main parameter observed was abdominal fat weight, supporting parameter was bodyweight and food consumption. Data was analyzed by varians analysis with Split Plot Design and continued withDuncan Multiple Range Test. The result was turmerin extract influences broiler’s abdominal fat. Increase anddecrease of abdominal fat are comparable with chicken body weight. On chicken 1 – 4 weeks of age, 200 mg/kgBW/day turmerin extract and 400 mg/kg BW/day turmerin extract increase chicken’s abdominal fat. On chicken 4 –7 weeks of age, 200 mg/kg BW/day turmerin extract increases chicken’s abdominal fat.

Metode Pengendalian Wideng (Sesarma spp) Hama Bibit Mangrove melalui Kegiatan Budidaya Kepiting Bakau Scylla spp

Hidayat, Jafron Wasiq

Bioma Vol. 13, No. 1, Tahun 2011
Publisher : Bioma

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Abstract

Reboisation is one strategy to anticipate global warming that most easily performed. This re-plantingactivity is very urgent to be conducted in mangrove areas, since the impact of global warming are very profound atthe coastal and lowland areas. Its implementation has been done, but failures are still high. One of the causes oftenfound in the field is pests. Its efficient and effective control are still faces various obstacles. Wideng (Sesarma) isvery often perform as pest to the mangrove propagules, therefore could affect the community structure. It is thereforeimportant to establish conceptual study and research on wideng control, especially those that are able to promoteparticipation and benefit the community. One potential control is to empower its natural predators, the mud crabScylla. The purpose of this research is to analyze the level of Scylla predation, namely of total prey consumed, preysize and prey density presentation. It is also intended to determine weight gain and predation behavior byScylla. Two units of the test cage were placed in the pond in Tapak Tugurejo Semarang with Complete RandomizedDesign (3 treatments and 5 replications). Data on predation tests are descriptively and statistically analyzed usingJMP software, whereas the study of behavior was analyzed descriptively.The results demonstrated that Scylla is able to prey and consume wideng namely the life ones. Scyllaperform no respecter of prey body size, since wideng of small body size (2-3 cm), preferably the same to themoderate size (4-5cm) and large size (> 5 cm). The level of consumption gives a high weight gain for Scylla,between 182 - 197% in just 6 days. In term of presentation of the treatment, showed that wideng really liked thepresentation of the feed lot at once rather than presenting a slightly but gradually. Finally, feeding on Scylla withwideng through cultivation, particularly in fattening purposes, using wideng is reasonable and flexible to be appliedfor fishermen. This concept, together with other control components (components of a dissertation study of theauthor), is very supportive on the wideng control whiles provide economic benefits to the society atonce.

Pertumbuhan Semai Jarak Pagar (Jatropha curcas L. ) Asal Biji Dan Stek Yang Ditanam Pada Jenis Tanah Berbeda

Prihastanti, Erma -

Bioma Vol. 13, No. 1, Tahun 2011
Publisher : Bioma

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Abstract

The successful of the growth of Jatropha curcas L. in seed yield is affected when the nursery, such as howthe provision of seedlings and the media used. Jatropha propagation is generally done through vegetativepropagation (cuttings) and reproductive (seed).. Several efforts to maximize the quality of seedlings of which is withthe selection of appropriate planting medium, as in the choice of soil. This study aims to assess the growth ofjatropha seedlings from seeds and cuttings in different soil types. Research conducted at the Laboratory of Plant TestBogor Agricultural University, in February - July 2006. Jatropha seedlings used a month old from seed and cuttingsfrom parent trees were 8 years old. The growing medium used in the form of latosol and podzolic soil. Observationof growth after two months of maintenance performed by observing the plant wet weight, leaf number and length ofseedling roots.The results showed the growth of jatropha seedlings influenced by seedling origin and type of soil. Jatrophaseedlings from seeds or cuttings are planted in latosol or atosol increased wet weight, root length and number ofleaves for two months of the nursery. The best growth from seeds of jatropha seedlings when planted in soil latosol,while seedlings from cuttings better if planted in soil podzolic.

Studi Awal Upaya Eksplorasi Agensia Imunokontrasepsi Untuk Regulasi Fertilitas Hewan Liar : Pofil Protein Selama Proses Implantasi Embrio Mencit (Mus musculus L.) BALB/c.

Sitasiwi, Agung Janika, Djaelani, Muhammad Anwar

Bioma Vol. 13, No. 1, Tahun 2011
Publisher : Bioma

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Abstract

Study on exploration of immunocontraception agents for wildlife fertility regulation has been conducted.The aim of this study was carried to find out the protein which functionable in mice embryos implantation. Theoutcome of this study should be applied to control the fertility of wildlife animals. This research conducted duringten months in BSF Laboratory of Biology Department FMIPA UNDIP and 3rd Unit of UGM LPPT. Adult femaleand male mice with 28 – 30 grams in weight were used as laboratory animals. Mice were divide into two groups, onegroup as positive control group without mated, the other were mated group. Mice handled and breeded in laboratorycondition. The precise day of pregnancy were determined with vaginal plug existences in female mice. Protein wereisolated from uterus on day 1st to 5th of pregnancy. Protein isolation and separation with electrophoresis were basedon Bio-rad manual. This study show that there are no differences in protein band between positive control group andpregnant group. These study suggested that protein determining embryo implantation process is regulatory proteinwhich it has expressed in short time and fast withdrawl.