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BIOMA
Published by Universitas Diponegoro
BIOMA (ISSN 1410-8801) adalah Majalah Ilmiah Biologi yang bertujuan mewadahi semua informasi hasil penelitian, telaah pustaka, makalah teknis, dan kajian buku, dari berbagai cabang ilmu Biologi. Diharapkan penerbitan ini dapat berkontribusi dalam penyampaian informasi ilmiah yang merupakan mata rantai proses kegiatan ilmiah. Terbit sejak tahun 1999.
Articles by issue : Vol. 12, No. 1, Tahun 2010
6
Articles
Aktifitas Inhibitor Alpha-Glukosidase Bakteri Endofit PR-3 yang Diisolasi dari Tanaman Pare (momordica charantia)

Pujiyanto, Sri -, Ferniah, Rejeki Siti

Bioma Vol. 12, No. 1, Tahun 2010
Publisher : Bioma

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Abstract

Some traditional medicinal plants are known to have efficacy as a medicine for diabetes. Active compoundsproduced by a plant can be derived from endophytic microbes that live in these plants. One way diabetes drugs workis to prevent digestion of complex carbohydrates into glucose so that glucose intake is reduced. Alpha-glucosidaseinhibitor is a compound that can prevent the digestion of carbohydrates, especially starch into glucose. This studyaimed to test the inhibitory activity of alpha gluosidase PR-3 isolate, an endophytic bacteria from Momordicacharantia. The results showed that the crude extract (supernatant) from PR-3 has the capability of the enzyme alphaglucosidase inhibition that is equal to 61.2% compared with positive control compound acarbose 1 mg / ml. Theresults also showed that the use of maltose as carbon source produce the highest an alpha glucosidase inhibitor(54,97%), followed by the starch (47.77%), glucose (31.97%), fructose (44.14%) and sucrose (27.7%).

Induksi Kalus dari Hipokotil Alfalfa (medicago sativa l.) secara in vitro dengan Penambahan Benzyl Amino Purine (BAP) dan α-Naphtalene Acetic Acid (NAA)

Hayati, Surya Kurnia, Nurchayati, Yulita -, Setiari, Nintya -

Bioma Vol. 12, No. 1, Tahun 2010
Publisher : Bioma

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Abstract

Alfalfa (Medicago sativa L.) is useful plant for treatment some diseases such as: cancer, diabetes, lupus, andhepatitis. Propagation of this plant in Indonesia face a problem which has no embryo. One method to propagate thisplant is by tissue culture or micropropagation. Callus induction is first step in micropropagation to produce calluswhich will be regenerated to become planlet. The aims of this research are to induce callogenesis from hipocotyl ofalfalfa with addition Benzyl Amino Purine (BAP) and α Naphtalene Acetic Acid (NAA), and to determine the propercombination of BAP and NAA to produce the optimal callus. The experiment has been conducted by using 12combination of BAP and NAA with Completely Randomized Design (CRD) in 4x3 factorial pattern by 5 replicates.Data were analyzed by ANOVA 95% Degrees of Freedom (DF). If there was significance result, it was followed byDMRT analyzed at 95 % DF. The result showed that combination of BAP and NAA was able to induce callogenesisfrom hipocotyl of alfalfa. The optimal callus was obtained in combination of BAP 0 ppm and NAA 2 ppm.

Ekohidrologi Konsep Pengelolaan Lingkungan Berkelanjutan

Soeprobowati, Tri Retnaningsih

Bioma Vol. 12, No. 1, Tahun 2010
Publisher : Bioma

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Abstract

Ecohydrology is the study on the interaction hydrological process or aquatic with biological dynamicspatially and temporarily. Ecohydrology promises to be a tool of the sustainable use of aquatic resources byecological analysis integrated with freshwater aquatic conservation. Methodologically, ecohydrology is amanagement tool using organism to control hydrological process and using hydrology to control biota. Basically,ecohydrology is water resource development that oriented on the capacity and ability to maintain it. Spatially, itrequires an understanding about the role of organism. Temporarily, it requires reconstruction on thepaleohydrology as a base of the water resource development that put global changes into account. Based on thesuccessful implementation of ecohydrological concept on Saguling Reservoir, it able to be implemented for anotheraquatic ecosystem.

Optimasi Produksi Inulinase isolat P 12 pada Tepung Umbi Dahlia ( Dahlia variabilis Wild ) dengan Variasi Konsentrasi Nitrogen Organik dan Waktu Inkubasi

Lunggani, Arina Tri, -, Wijanarka -, Kusdiyantini, Endang -

Bioma Vol. 12, No. 1, Tahun 2010
Publisher : Bioma

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Abstract

Efforts to address health problems mainly related to the digestive tract is by consuming one prebiotic, egfruktooligosakarida (FOS). FOS is a prebiotic that one species can be produced from the hydrolysis of inulin usinginulinase enzyme. Isolate P12 is an isolate that has been proven to have high inulinase activity on standard mediuminulin production. Inulinase production increase can be done by adding a source of organic nitrogen in the form ofyeast extract in medium. The results indicate that the best on the concentration of nitrogen concentration P2 (Yeastextract 0.25%) with the activity of 0.7983 IU, while the best 12-hour incubation time with the activity of 0.7899 IU.Likewise for the best interaction P2 T2 treatment with inulinase activity of 0.9025 IU.

Perubahan Struktur Pembuluh Xilem Akar Kakao (Theobroma cacao L.) dan Gliricidia sepium pada Cekaman Kekeringan

Prihastanti, Erma -

Bioma Vol. 12, No. 1, Tahun 2010
Publisher : Bioma

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Abstract

Supply of water into leaves depends on the existence of water in xylem. Root system of plants will be affectedfirst if the plants exposed to stress of water shortage. The structure of xylem vessels is an important factor indetermining the presence of embolism induced by drought and varies from one type to another. Research on cocoaagroforestry tree Gliricidia sepium is used as cover crops. The purpose of this study was to analyze changes in thestructure of xylem vessels including area ratio, diameter and hole diameter of cacao root xylem vessels and G.Sepium in drought conditions.Drought stress on the cacao tree and G.sepium performed using EFT systems (throughfall displacementexperiment), which runs from February 2007 - February 2008. Cacao tree used in this study was 5 years old, while6-year-old G.sepium tree. Location of the experiment were divided into six plots comprising three control plots andthree plots roofing. Each plot was taken six and three cacao trees G.sepium tree, where each tree root system wastaken three pieces with diameters of 3-5 mm at 20 cm soil depth.Research results showed that drought stress during 13 months did not affect xylem area ratio, diameter ofxylem cells and xylem vessels, root diameter hole and G.sepium cocoa. However, structural information can beobtained from the root xylem of cocoa have a cell - more xylem cells and pore holes smaller than the root G.sepium.This is shown by the average ratio of the area with an overall area of the root xylem (k) on cocoa roots are ± 0.70while in the root G.sepium ± 0.49. Average diameter of the root xylem cells cocoa ± 1 μ in diameter, length 50-10μm ± vessels and vessel diameter hole ± 0.25 μ. Meanwhile root xylem cells G.sepium shows average diameter ofthe root xylem cells is 1-3 μ, ± 15-25 μ long vessels and vessels holes ± 0.5 μ rang

Tree Species Composition and Distribution in Sungai Lalang Forest Reserve, Selangor, Malaysia

Sasse, Fadal Farag K, hendrarto, Boedi -, Izzati, Munifatul -

Bioma Vol. 12, No. 1, Tahun 2010
Publisher : Bioma

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Abstract

Species richness, species composition, height, diameter class structure and tree species diversity wereexamined in a 1 plot in lowland tropical rain forest in Sungai Lalang forest reserve, Selangor, Malaysia. However,some of trees with diameter of breast height (DBH) of 5 cm and above were measured and recorded to be analyzed.As a result, all species at the study site were compared with some results which were found equaled abundantaccording to Evenness Index that gave a value of 0.922; Margalef’s Index reflected a value of 17.01. Chaetocarpuscastanocarpus (Euphorbiaceae) was the most important species with an IVI (Important Value Index) of 4.643%,while Euphorbiaceae was the dominant family for the study area with IVI of 14.02%. The recommendation are thestudy area should be managed and protected in the right way to ensure the continued existence and conservation ofMalaysia natural resource