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Jurnal Penelitian Teh dan Kina
Jurnal Penelitian Teh dan Kina is an national journal providing rapid publication of peer-reviewed articles concerned with tea and cinchona commodities based on the aspects, agronomy, plant breeding, soil science, crop protection, postharvest technology and social economy. Papers dealing with result of original research on the above aspects are welcomed with no page charge.
Articles by issue : Vol 19, No 2 (2016)
10
Articles
Analysis of Tea Rhizosphere Bacterial Community at the Seedling Stage Using Terminal Restriction Fragment Length Polymorphism (TRFLP) Techniques

Susilowati, Dwi Ningsih, Fauziah, Fani, Pranoto, Eko, Hidayat, Ernin, Setyowati, Mamik, Rachmiati, Yati

Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Bio-imunizer contains an active compound of  Chryseobacterium sp. and Bacillus sp. has been developed by PPTK Gambung. This formula has positive effect on the growth of tea plants also potentially increasing resistance of the plant. The purpose of this study was to determine the effects of bacteria in Bio-imunizer to the rhizosphere bacterial communities as well as the consistency of its existence after application on tea plants at the nursery stage. The technique used in this research is Terminal Restriction Fragment Length Polymorphism based on metagenomic and culture dependent approaches. The value of relative abundance, Shannon diversity index, Pielous evenness index, and Simpson dominance index were calculated. Based on the T-RF profiles of rhizosphere bacterial communities show that Chryseobacterium sp. and Bacillus sp. which is the active compound of  Bio-imunizer consistently found in the tea plant rhizosphere. Application of Bio-imunizer can increase the diversity of rhizosphere bacterial community without affecting the communities that already exist.

Exploration and Effectiveness Test from Some Tea Indigenous Potassium Soluble Bacteria

Pranoto, Eko, Wulansari, Restu, Athallah, Faris Nur Fauzi, Lestari, Fitrianti Widya

Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Tea is a plant that benefits for health. The growth of tea plant was influenced by several factors, including superior clones and nutrient availability in soil. One of the most important macro nutrients is potassium. However, the availability of potassium is low because of retention on the soil. One of the methode for absorbed by plant was used Potassium Solubilizing Bacteria (PSB) with its organic acid function. The aim of the study was to explore and determine the density of colonies (DC) and the potential of Tea Indigenous PSB. Inoculum source from tea rhizosphere and rhizoplan of GMB 7, GMB 9 and TRI 2024 clones. The experimental design used was completely randomized design with six treatments and four replications, i.e. : GMB 7 Rhizosphere; GMB 7 Rhizoplan; GMB 9 Rhizosphere; GMB 9 Rhizoplan; TRI 2024 Rhizosphere; and TRI 2024 Rhizoplan. Ten isolates with the highest halozone was tested by the Dissolution Index (DI) of the PSB. The result showed that the highest DC was GMB 9 rhizoplan by 10.9 x 105 CFU/g and the lowest was TRI 2024 rhizoplant by 3.1 x 105 CFU/g. The DC on GMB clones series showed that rhizoplan was 14,29% higher than rhizosphere. There is no significant difference of DI for PSB colonies from all clones. From that clones, we were obtained 10 isolat that have the highest halozone, that were 2 isolates from rhizosphere and 8 isolates from rhizoplan. The average of DI rhizosphere isolates was 119,9% higher than rhizoplan.

The growth test of tea clones (Camellia sinensis) from cross pollination first generation clones

Rahadi, Vitria Puspitasari, Syahrian, Heri, Sriyadi, Bambang

Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Efforts to improve the productivity of tea needed new high yielding clones were obtained from the cross and easily propagated vegetatively. Preliminary testing of the clones  needs to be done to determine the growth of cuttings and rooting properties of tea clones from hybridization which are selected by potential production. This study used a Randomized Complate Block Design with three replications. The treatments consist of 14 clones from crosses and GMB 7 as clone comparison. The data is analyzed by using ANOVA and Scott Knott test with the level of 5%. The observations are intended to look at the growth of roots and shoots by dismantling seedling in polybag predetermined randomly at each observation. The results showed that the clones tested had good growth with a low mortality rate, and easily propagated vegetatively. The S / R ratio also showed shoot growth more faster than the growth of root. This character is advantageous because the most appropriate selection criteria for planting in the field.

Xantine oxidase inhibition activity of infusion and ethanol extract of white tea

Rustamsyah, Ardi, Islami, Sarah Nur, Fitriana, Fitriana, Kusmiyati, Mimin

Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Research of xantine oxidase enzyme inhibition activity of infusion and ethanol extract of white tea were done. The aim of this research was to evaluate  xantine oxidase inhibitory activity of infusion and ethanol extract of white tea. Phytochemical screening on dry tea, infusion and ethanol extract of white tea showed that all samples contained alkaloid, flavonoid, quinone, tannin and tritherpenoid/ steroide. The IC50 value of samples were 76,64 μg/ml and 31,11μg/ml respectivelly. This study used allopurinol as standard with IC50 was 0,60 μg/ml.

Vegetative growth ability of artificial cross pollinated of tea clones

Syahrian, Heri, Rahadi, Vitria Puspita, Ruhaendi, Endi, Santoso, Budi

Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Vegetative propagation is common system that carried out in tea propagation. Seedling propagation was not reliable due to time consuming and phenotypic variability in seed plant. Despite yield potential, good quality, pest and disease resistant characters, the easy of vegetative propagation is very important character in tea breeding process. The aim of this study is to selected clones that resulted by artificial pollination for an ability of vegetative propagation character. Plant materials that tested in this experiment were the seed from the vegetative propagation of 14 clones, and GMB 7 clone as a control. Randomized Block Design was used in this experiment by 3 replications. The data analysis was ANOVA and Scott Knott test. The result of the experiment showed that clone number 41, 45, 38, 149, X5, X3, 122, 78, and 44, were the easiest clones to be vegetative propagated.

Inhibition activity of α-glucosidase by infusion and ethanol extract of white tea and stevia combination

Trinoviani, Elvi, Kholisoh, Ai, Ar-Rifa, Nisa Fitriani, Rustamsyah, Ardi

Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Ethanol extract of white tea and its stevia liquor was observed for α-glukosidase inhibition. The research was divided into some steps;  white tea and stevia combination hedonic sensory test, phytochemical identification, and α-glucosidase inhibition activity. The result of hedonic sensory test incline towards formula 2 which consist 75% of white tea and 25% stevia. Further, this formulation was extracted by ethanol for α-glucosidase inhibition activity test. Formulation 2 liquor tested for enzyme inhibition activity showed IC50 value was 36,96 µg/ml, better than ethanol extract and acarbose. Phytochemical identification presented that both preparations contained alkaloids, flavonoids, saponins, tannins, triterpenes/steroid, and quinones.

Antidiabetic mellitus activity of low grade black tea in alloxan induced male albino mice

Deswati, Dytha Andri, Maryam, Zahra Nur

Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Tea (Camellia sinensis [L]) has a very potential character of quality and biological activities. Potential activity of black tea such as antidiabetic been proven by several studies. Grade of black tea was classified based on colour, shape, size and density of particles.  This study aims to evaluate the most optimal antidiabetic activity of low grade black teas;BT II, DUST III, BBL and BTL in alloxan induced male albino mice. The results showed that liquor of black tea grade DUST III has blood glucose lowering activity with the most optimal percentage reduction 49.07% with a significant difference in the positive control group (p <0.05).

The effect of drought period on attack intensity of Empoasca sp and blister blight in Gambung tea plantation

Rezamela, Erdiansyah, Fauziah, Fani, Dalimoenthe, Salwa Lubnan

Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

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Abstract

El-Nino had a significant impact on micro climate change including in rainfall, temperature and air humidity in Gambung Tea Plantation. In the dry season 2015, Gambung experienced four dry months that affect attack intensity of Empoasca and disease intensity of Blister Blight. This study aimed to determine the relationship between micro climate parameters and attack intensity of Empoasca and disease intensity of Blister Bright. Micro climate observation data were taken from Davis Automatic Weather Station (AWS) including rainfall intensity (mm), relative humidity (%) and maxium air temperature (0C). Attack intensity of Empoasca sp and Blister Bligh were observed by counting the number of healthy P+3 shoots as well as infected shoot from 200-500 gram of wet shoot sample randomly taken from container net at the plucking time. The results showed that the intensity of Empoasca decreased from 16.49% to 12.90% in the dry months from July to October 2015. The disease intensity of Blister Blight only occurred in July. There was a polynomial relationship among rainfall, temperature and humidity to the attack intensity of Empoasca with R2 values of 0.71, 0.77, 0.87, respectively the decrease of attack intensity occurred at ranfall intensity >140 mm, temperature >28°C and humidity >80%. Meanwhile linear relationship occurred among rainfall, temperature and humidity to the disease intensity of Blister Blight with R2 values of 0,98; 0,64; and 0,77, respectively the increasing of rainfall and humidity caused the increasing of disease intensity of Blister Blight. However disease intensity of Blister Blight decreased with increase in ambient temperature.

The study of transparent soap making from virgin coconut oil-based with the addition of white tea extract as an active ingredients

Widyasanti, Asri, Hasna, Anditya Husnul

Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Transparent soap was made using high quality materials that beneficial for skin health and beauty. The main ingredient of the transparent soap was virgin coconut oil (VCO), with the addition of white tea extract which was able to increase the benefits of transparent soap. This study aimed to create formulation of the transparent soap, to find the addition of the white tea extracts to produce transparent soap and to study the characteristics of the transparent soap after the addition of white tea extracts. The treatments in this study were the addition of white tea extracts A = 0% (w / w), B = 0.5% (w / w, C = 1.0% (w / w and D = 1.5% (w / w). Observations on the transparent soap were including chemical properties, physical properties of soap, antibacterial and organoleptic test. The results of the analysis showed that all transparent soap formulas met the requirements of solid soap SNI 06-3532-1994, except for the total fatty acid. The formula of transparent soap with treatment B (addition of white tea extract 0.5% w / v) was the best product with organoleptic test results reached 43.34%. The analysis results of those soap were 11.47% of water content and evaporating substances, 37.97% of total fatty acid, 0.519% of free fatty acid, 2.24% of unsaponified fraction, pH value of 9, hardness 0.0077 mm/g/sec, the foam stability of 76.88% and the activity of antibacterial inhibition diameter of 10.7 mm, respectively. This technology could be applied in the production of transparent soap with the addition of white tea extract and to be developed into some industrial scale.

The effect of climate change on rainfall pattern and deficit of water in tea plantation

Dalimoenthe, Salwa Lubnan, Apriana, Y, June, T

Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Climate change has been influencing rainfall pattern so that it would be necessary to see the impact of that changed on tea plantation. The experimental area coverage lowland (600 m asl), midland (800-1000m asl) and highland (>1.000 m asl) tea plantation and each altituted represented by three tea estate in West Java. The rainfall data collected since 2005 up to 2014 from each estate and water deficit has been count through the method develop by Wijaya (1996). The results showed that the rainfall pattern has been changed by La-Nina and El-Nino during 2005-2014 in tea estate either in lowland, midland or highland in the last decade. The climate change caused  rainfall decreasing and increasing on dry month (the rainfall < 100 mm). Eventhough on 2009 there is an significantly increasing of the rainfall but after 2009 until 2014, the rainfall tend to decrease. After El-Nino on late 2009 and early 2010, lowland tea estate on Subang Regency facing water deficit until 5 months with R (defisit water index) far below 1 even there is no El Nino. The tea plantation at midland area (Cianjur Regency) facing 5 months water deficit per year, but the R index close to 1. While in highland tea plantation (Bandung Regency), the water deficit only happend on certain month on certain year although there is a month with zero rainfall. Water deficit could be happend because of runoff on soil surface stimulate by low ability of soil to keep the water.