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Jurnal Penelitian Teh dan Kina
Jurnal Penelitian Teh dan Kina is an national journal providing rapid publication of peer-reviewed articles concerned with tea and cinchona commodities based on the aspects, agronomy, plant breeding, soil science, crop protection, postharvest technology and social economy. Papers dealing with result of original research on the above aspects are welcomed with no page charge.
Articles by issue : Vol 16, No 1 (2013)
5
Articles
The effects of organic planting medium on growth and root formation of tea seedling at early stage of tea nursery

Dalimoenthe, Salwa Lubnan

Jurnal Penelitian Teh dan Kina Vol 16, No 1 (2013)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Original Source | Check in Google Scholar | Full PDF (392.449 KB)

Abstract

Tea plants commonly propagated through vegetative propagation using single node leaf cutting and planting on mixed of topsoil and subsoil. From time to time, it is difficult to get suitable soil for nursery and the activity could disturb ecosystem. In addition, the medium weight would become a constraint in transporation of tea seedling. To overcome these problems, an alternative planting medium of tea seedling has to be explored to reduce the use of soil. In this research, various organic materials have been tested to know their effect on growth and root formation of tea seedling at early stage of tea nursery. The experiment arranged in a complete randomized design with nine treatments, replicated three times. Treatments tested were combination of organic matter (cocopith, rice husk, sawdust, and peat soil) with top soil (15% and 25%). Cow dung, as organic fertilizer, was added to enrich planting medium. Results shows that cocopith, rice husk, sawdust, and peat soil enriched with cow dung resulted the same with top soil medium (convensional planting medium) on growth and root formation of tea seedlings at early stage of tea nursery.

Micrografting technique in cinchona (Cinchona ledgeriana Moens) plants propagation

Dalimoenthe, Salwa Lubnan

Jurnal Penelitian Teh dan Kina Vol 16, No 1 (2013)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Original Source | Check in Google Scholar | Full PDF (390.118 KB)

Abstract

In Indonesia has been found 11 cinchona species but only two species recognized as an economically important Cinchona succirubra known as resistant plant to root disease while Cinchona ledgeriana quinine high content. To put together those two characters from mostly through grafting it took twelve months. Micrografting is one of the propagation techniques through tissue culture. Micrografting has already been used in wide spread for plants propagation either only for research or as mass product. Hopefully, the use of micrografting to obtain cinchona propagation would be short more or less 10 months and provide plants as mass production economically. The result showed that micro grafting at V shape could be used as the propagation method on cinchona species besides bud multiplication through tissue culture. MS medium is the best medium for cinchona micrografting. If there is no IBA on rooting media, there is no root formation from the plants. The increasing of IBA from 1 up to 4 mg/l in media, will increase also the number of rooting plants.different plant material as scion and stock and different concentrarions (0,1,2,3,4 mg/l) of IBA gives the significantly different respons on rooting formation. The increasing of IBA on media will increase the number of rooting plants also. The highest rooting formation are found on medium with 3 dan  4 mg/L  IBA. The combination between C. ledgeriana-C. succirubra, C. ledgeriana-C.ledgeriana, C. succrirubra-C.succirubra gives significantly difference on the growth dan development of root. The use of C. ledgeriana as a root stock doesn’t showed good rooting formation. The combination between CSA/QRC205 gives the highest number of leaves result compare wth others combination on as much as 9,8. The first stage of acclimatization on culture room with temperature 25-270C and followed by acclimatization on plastics house covered with paranet 70%. In general in vitro planlet cinchona from micrografting growing well. By the end of observation (8 weeks) growing percentage reach 90%.  

The effectiveness of formulation of brenuk botanical insecticide on Empoasca flavescens on tea

Sucherman, Odih

Jurnal Penelitian Teh dan Kina Vol 16, No 1 (2013)
Publisher : Research Institute for Tea and Cinchona

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Abstract

An experiment to find out the effectiveness of formulation of brenuk botanical insecticide on Empoasca flavescens on tea was carried out at Pasir Sarongge Experimental Garden, Research Institute for Tea and Cinchona, from Juni up to September 2012. The experiment was designed in randomized complete block design with eight treatments and three replications. The treatment was consist of: (1) brenuk insecticide at 0,5 litre of EC formulation/ha, (2) brenuk insecticide at 1,0 litre of EC formulation/ha, (3) brenuk insecticide at 1,5 litre of EC formulation/ha, (4) brenuk insecticide at 0,5 litre of WP formulation/ha, (5) brenuk insecticide at 1,0 litre of WP formulation/ha, (6) brenuk insecticide at 1,5 litre of WP formulation/ha, (7) marigold insecticide at 1,5 litre of EC formulation/ha, and (8) control. The water spray volume used was 300 litre/ha. The observation parameters were the attack intensity of E. flavescens, the population of E. flavescens on the production of tea shoots. The experiment result indicated that the application of brenuk insecticide at 0,5 litre/ha in EC as well as WP formulations were efective in the decreasing of attack intensity of E. flavescens, as well as the population of Empoasca to the extend of 69,99% and 63,63% respectively. Indirectly, these treatments could increase the shoot production of tea plant.

Inhibitory activity of Escherichia coli by type of teas and its liquors

Rohdiana, Dadan, Arief, Dede Zainal, Budiman, Arista

Jurnal Penelitian Teh dan Kina Vol 16, No 1 (2013)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Original Source | Check in Google Scholar | Full PDF (348.671 KB)

Abstract

Study of analysis polyphenol content on tea, liquor, and its activity as antibacterial on Escherichia coli has been done conducted in Laboratory of Research, Department of Food Technology, Pasundan University, and Laboratory of Chemistry, Department of Chemistry, Faculty of Mathematic and Natural Science, Padjadjaran University. The result of this research showed that green tea sample has the highest polyphenol content, i.e. 23,18% followed orthodox and CTC (crushing tearing curling) black tea samples i.e. 14,23%  and 13,93% respectively. Activity of inhibitory on E. coli showed that ratio tea and water 2 : 50 w/v has the best inhibition i.e. 117,71 mm2.

(1,1-Diphenyl-2-Picrylhydrazyl) free radical scavenging activity of white tea base on temperature and period of brewing

Rohdiana, Dadan, Arief, Dede Zainal, Somantri, Mamay

Jurnal Penelitian Teh dan Kina Vol 16, No 1 (2013)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Original Source | Check in Google Scholar | Full PDF (332.03 KB)

Abstract

Research of 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) free radical scavenging activity has been done conducted on Laboratory of Quality Assesment, Research Institute for Tea and Cinchona, and Laboratory of Chemistry, Chemistry Department, Faculty of Matematic and Natural Science, Padjdjaran University. The result showed that among four white tea from different estates, total polyphenol content on Gambung White Tea has the highest. Total polyphenol content of Gambung white tea i.e 25,52%. Brewing process on 95oC for 9 minutes obtained the highest polyphenol content on tea infusion i.e. 6,01% and the lowest EC50 i.e. 35,41 µl/ml. This research showed that presence of positive correlation between total polyphenol and antioxidant activity base on EC50. The correlation value in this research i.e. 0,9066.