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Jurnal Penelitian Teh dan Kina
Jurnal Penelitian Teh dan Kina is an national journal providing rapid publication of peer-reviewed articles concerned with tea and cinchona commodities based on the aspects, agronomy, plant breeding, soil science, crop protection, postharvest technology and social economy. Papers dealing with result of original research on the above aspects are welcomed with no page charge.
Articles by issue : Vol 15, No 2 (2012)
5
Articles
Activity DPPH (1,1-diphenyl-2-picrylhidrazyl) free radical scavenging of some ready to drink teas on

Rohdiana, Dadan, Shabri, Shabri

Jurnal Penelitian Teh dan Kina Vol 15, No 2 (2012)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Research of free radical scavenging activity of some ready to drink tea (RTD-Tea) was carried out. Generally, the result showed that tea beverages have a good of scavenging activity against DPPH free radical. T-AR was RTD-tea which has the stronger sca­venging activity against DPPH free radical. This RTD-tea has a low of EC50 value i.e. 63,55ml/ml. The scavenging activity followed were T-ES (63,95ml/ml), T-UP (65,75ml/ml), T-RO (86,85ml/ml), T-LI (91,15ml/ml), T-TA (107,53ml/ml), T-NG (125,36ml/ml) and T-AK (756,14ml/ml) respectively.

Analysis of leaves morphology similarity of several first generation tea clones

Sriyadi, Bambang

Jurnal Penelitian Teh dan Kina Vol 15, No 2 (2012)
Publisher : Research Institute for Tea and Cinchona

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Abstract

As an effort to conserve tea clone accessions of first generation from genetic erosion, arrangement germplasm in one particular block/area to be easily monitored and managed, is needed. Ideally, tea germplasm collection should consist of a few clone accession number but high genetic variability. To select representative clones that will be conserved in new germplasm collection, leaves morphology similarity has been analysed by cluster analysis using average method. The results showed that by using leaves morphology parameter, from 50 tea clone accession of first generation coming from several tea plantations, it could be reduced into 25 clone accessions from seven clusters, which each has high similarity. The first cluster with 60% similarity could reduced accession from 15 to 6 clone. The second with 74,8% similarity from 25 to 13 clones. The third with 74,8% similarity, from 4 to 2 clones. The fourth with 61,3% similarity, and seventh with 82,1% similarity, both could reduce from 2 clones to 1 clone. To design a tea germplasm collection with 50 accessions of genetic variability, it could represented by 25 clones with low similarity, i.e PG 3, KP 1, Skm 18, Cin 69, Cin 67 dan Cin 58 as the first cluster representatives, Mel 101, PG 4, Pam 15, PG 5,  PG 11, GP 2, BD 13, SA 49, Cin 66, Mal 16, Bks 6 and Sin 5 the second cluster representatives;   Mel 108 and Cin 13 as the third cluster representatives; and Cin 15, Cip 37, GH 5, as well as GP 4 as the fourth, the fifth, and the sixth representatives, respectively.

The effect of NPK compound fertilizer (27%:6%:10%) compared to conventional fertilizers on productive tea plants of GMB 7 clone in the Andisols soil type

Rahardjo, Pudjo, Rachmiati, Yati, Salim, Arkat Agus

Jurnal Penelitian Teh dan Kina Vol 15, No 2 (2012)
Publisher : Research Institute for Tea and Cinchona

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Abstract

The effectiveness application of compound fertilizer NPK (27%:6%:10%) to increase  tea shoots had been tested in Experimental Garden of Research Institute for Tea and Cinchona Gambung, Bandung, West Java, from January 2006 to March 2007. Experi­mental design used was randomized block design with 6 treatments with five replication. The plot sizes were 20 x 20 m = 400 m2/plot. The tea clone tes­ted was GMB 7. The appli­cation of compound fertilizer NPK (27%:6%:10%) were twice a year (50%-50%) and conventional fertlization were 4 times a year (20%-30%-20%-30%). The composition of  treatment was as follows: (A) 500 kg, (B) 600 kg, (C) 700 kg, (D) 800 kg/ha/year, and (E) conventional fertilizer recommended dosage of the Research Institute  for Tea and Cinchona Gambung (480 kg urea + 357 kg ZA + 260 kg SP 36 + 300 kg KCl + 278 kg  kieserit/ha/year), and (F) control (no fertilizer treatment). Parameters  observed were: 1. soil and leaf analysis at before and after treatment, 2. weighting the potential and the yield components each time of plucking, and 3. technoeconomic analysis. The results showed that: statistical analysis of the components as well as potential yield, and nutrient analysis (N, P, K, Mg and Zn) of the mother leaves of GMB 7 clones and the soil demon­strated the significantly differences among the treatments of all the various doses of compound fertilizer NPK and conventional fertilizer application to control (no fertilizer treatment). Meanwhile, there were no significantly differences among the result of  the doses of compound fertilizer NPK, to the conventional fertilizer applications. Further­more, dose of compound fertilizer NPK (27%:6%:10%) for produc­tive tea plant was recommendated at 700 kg/ha rate and was equivalent to 189 kg N/ha/year or 63% of the dose of conventional fertilizer, but could provide equivalent result to conventional fertilizer application, and not only could save the buying cost of conventional fertilizer  (urea, ZA, TSP/SP-36, KCl / MOP and kieserit) but also the cost of application cost in the field.

The effectiveness of the formulation of botanical insecticide marigold (Tithonia diversifolia) on Empoasca flavescens, a main pest on tea plant

Sucherman, Odih

Jurnal Penelitian Teh dan Kina Vol 15, No 2 (2012)
Publisher : Research Institute for Tea and Cinchona

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Abstract

A research to know the effectiveness of formulation of botanical insecticide marigold (Tithonia diversifolia) on E. flavescens has been carried out at a green house of Research Institute for Tea and Cinchona (RITC), as well as at Pasir Sarongge Experimental Garden of RITC, Cianjur, West Java, from September up to November 2011. At both experimental sites, the experimentations was designed in a randomized complete block (RCB) with  six treatments, replicated four times. Treatments tested were formulation of marigold extract 10% and 15%, each at doses of 0.5 and 1.0 l/ha, standard chemical insecticide, and control. All treatments were applied by spraying, using one litre-hand sprayer at green house, and using knapsack sprayer with spraying volume 300 l/ha in feld. At green house, parameter observed was mortality of E. flavescens. Mortality at formulation of marigold extract 15% (92%, in average) was higher than at formulation of marigold extract 10% (76%, in average) while in field were attack intensity of E. flavescens, tea shoot production, and phytotoxicity. Results showed that at green house, formulation of marigold extract 10% and 15% at doses of 0.5 dan 1.0 l/ha were effective to result in high mortality of E. flavescens. While in field the formulations at both doses were effective to suppress attack intensity of E. flavescens. However, the effectiveness of 1.0 l/ha doses was higher than 0.5 l/ha doses, which comparable with standard chemical insectiside treatment. The formulations at both doses indirectly also could increase shoot production, and did not cause phytotoxicity  on tea leaves/shoots.

Analysis of individual cathecin from green tea extracted and fractionated by column chromatography

Rohdiana, Dadan, Shabri, Shabri

Jurnal Penelitian Teh dan Kina Vol 15, No 2 (2012)
Publisher : Research Institute for Tea and Cinchona

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Abstract

A study determination of solvent and eluent on extraction process and fractination of individual cathecin especially epigallocathecin gallate (EGCG) by column chromato­graphy method. This study devided on two steps, i.e. determination of raw material, sol­vent, process optimation for extraction and fravtination of green tea extract, evapo­ration and drying. Characterization of polyphenol conducted by visible spectro­photo­meter, analysis of individual cathecin by HPLC. This study was did in Laboratory of Processing Technology and Laboratory of Testing for Tea and Cinchona, Research Institute for Tea and Cinchona, at September 2007 up to March 2008. Result of this study shown that ratio of solvent aceton and water 7:3 v/v, ratio of tea and solvent 1:15 w/v for 15 minutes at 55-60ºC obtained 53.39% polyphenol on extract and yield gradiently produce extract with purity of cathecin is 94% b/b, EGCG 64.03% from feed with cathecin content is 82.96% w/w and EGCG 42.34% w/w.