JURNAL SAINS INDONESIA
Vol 42, No 1 (2018): Jurnal Sains Indonesia

Pengaruh Suhu pada Ekspresi Pretrombin-2 Manusia Rekombinan di Escherichia coli ER2566


Silaban, Saronom, Simorangkir, Murniaty, Maksum, Iman Permana, Subroto, Toto, Hasan, Khomaini



Article Info

Publish Date
22 Sep 2018

Abstract

Human recombinant prethrombin-2 (rhPT-2) was expressed in Escherichia coli. Previous studies reported that the expression of rhPT-2 in E. coli tends to formed inclusion body. This study aims to determine the effect of temperature on the expression of rhPT-2 in E. coli ER2566. This study begins with the isolation of recombinant plasmids, competent cell preparation and transformation of competent cells of E. coli ER2566 strain, and rhPT-2 gene expression with various induction temperatures 12, 18, 22 and 30°C. Characterization results showed that the induction temperature of 22°C was the optimum temperature of rhPT-2 gene expression as a soluble fraction in E. coli ER2566 with IPTG concentration 0.1 mM. These results prove that the temperature affects the expression of rhPT-2 in E. coli ER2566.


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Original Source : http://jurnal.unimed.ac.id/2012/index.php/jsi/article/view/10700
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