07 Mar 2018
BACKGROUND: Paracetamol may cause apoptosis in the renal tubules. Moringa leaf (Moringa oleifera. Lam) has been shown to have antioxidanteffects due to its flavonoids. In addition, flavonoids also have biological effects to modulate the signal-transduction pathway. OBJECTIVE: to investigatethe effect of Moringa oleifera Lam extract on Bcl-2 and Bax expression on paracetamol-induced renal tubular injury.METHODS: in this post-test only control group design study, 24 rats were divided into 4 groups: AqC-G (aquades), EtC-G (70% ethanol), MoL1-Gand MoL2-G (moringa leaf extract at the dose of 1.075 g/Kg BW and 2.150 g/Kg BW respectively). Expression of Bcl2 and Bax was determined usingimmunohistochemical staining. The data on the number of Bcl2 and Bax expressions tested with One Way Anova followed by the Post Hoc LSD test.RESULTS: Post Hoc LSD test showed that the expression of Bcl-2 in the experimental groups ((MoL1-G (9.1) and MoL2-G (13,683)) was significantlyhigher compared to that of controls (p<0.001). Bax expression in the experimental groups (MoL1-G (6,85) and MoL2-G (2,633)) was significantly lowercompared to that of controls, p <0.001.CONCLUSION: The administration of Moringa leaf extract at the dose of 1,075 and 2,150 increases Bcl-2 expression and decreases Bax expression inparacetamol-induced renal tubular injury.
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