Vol 7, No 7 (2011)

INDUKSI APOPTOSIS EKSTRAK ETANOL CIPLUKAN (Physalis angulata L.) PADA SEL KANKER LEHER RAHIM HeLa MELALUI PENEKANAN EKSPRESI Bel-2


Indriyani, Luthfia ( Cancer Chemoprevention Research Center Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta. ) , Darma, Andita Pra ( Cancer Chemoprevention Research Center Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta. ) , Nugroho, Perdana Adhi ( Cancer Chemoprevention Research Center Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta. ) , Hermawan, Adam ( Cancer Chemoprevention Research Center Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta. ) , Meiyanto, Edy ( Cancer Chemoprevention Research Center Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta. )



Article Info

Publish Date
15 Jan 2013

Abstract

The incidence of cervical cancer continue to rise encourage a variety of studies to explore the plant as chemopreventive. Ciplukan (Physalis angulata L.) is one of potential plant as chemopreventive agent by regulating cell proliferation, cell cycle and apoptosis. In this study the potential effect of ciplukan ethanolic extract as inducers of apoptosis in human cervical carcinoma HeLa cell line wil be futher investigated. Induction of apoptosis was evaluated by Double Staining methods and Immunocytochemistry. Apoptosis observations qualitatively indicate the occurrence of early apoptosis marked by orange fluorescence and apoptosis bodies because of cell fragmentation. Then the expression of Bcl-2 was observed to know molecular mechanism of apoptosis. Immunocytochemistry experiment showed that Bcl-2 as antiapoptosis protein was inhibited in comparison with control cells. These data indicated that ciplukan ethanolic extract (P.angulata L.) could induced the apoptosis Hela cell line by downregulation of Bcl-2. Study of ciplukan shoud be developed to support ciplukan to be effective anticancer agents.


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