Journal of the Indonesian Medical Association
Vol. 61 No. 7 July 2011

Decreased Oxidative Stress and Apoptosis post Intermittent Hypobaric Hypoxia Exposures in Sprague Dawley Male Mice Cardiac Cells

Hidayat, Achmad ( Institute of Aviation Medicine Dr Saryanto, Indonesian Air Force, Jakarta ) , Wiradisastra, Kahdar ( Faculty of Medicine Universitas Padjadjaran, Bandung ) , Hernowo, Bethy Setiawaty ( Faculty of Medicine Universitas Padjadjaran, Bandung ) , Achmad, Tri Hanggono ( Faculty of Medicine Universitas Padjadjaran, Bandung )

Article Info

Publish Date
31 Jul 2011


Aviators often experience intermittent hypobaric hypoxia (IHH) which may cause apoptosis. This study was aimed to analyze the effects of IHH on oxidative stress and apoptosis. Experimental study conducted in February-April 2010 consisted of one control group and four exposed groups of male mice Sprague Dawley. Each group consisted of 7 mice. The control group did not have IHH. The exposed groups (with an interval of one week) had once, twice, three, or four times IHH using a chamber flight. All exposed groups were treated hypobaric equivalent to: 35 000 ft altitude (one minutes), 25 000 ft (five minutes), and 18 000 ft (25 minutes). Protein MnSOD and Caspase-3 expression on mice heart cell were detected by Western Blot methods. In regard to protein MnSOD expressions, subjects with four time IHH exposures had likely similar level of oxidative stress (p=0.4057) compared with control groups. However, in term of protein Caspase-3 expressions, subjects with one, two times IHH exposures had increased apoptosis (p=0.0000; p=0.0001 respectively); three and four time exposures had decreased apoptosis (p=0.0187; p=0.0001 respectively). In conclusion, oxidative stress and apoptosis cell started to decrease after three-time IHH exposures in Sprague Dawley male mice. J Indon Med Assoc.2011;61:289-92.Keywords: intermittent hypobaric hypoxia, MnSOD, Caspase-3, Sprague Dawley Male Mice

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