Indonesian Journal of Cancer Chemoprevention
Vol 3, No 2 (2012)

Antiproliferative Effect of Ethanolic Extract Eugenia uniflora Lam. Leaves on T47D Cells


Ismiyati, Nur ( Department of Pharmacy Politeknik Kesehatan Bhakti Setya Indonesia Yogyakarta ) , Putri, Dyaningtyas Dewi Pamungkas ( Cancer Chemoprevention Research Center Faculty of Pharmacy Universitas Gadjah Mada ) , Kusumastuti, Siska Andrina ( Cancer Chemoprevention Research Center Faculty of Pharmacy Universitas Gadjah Mada ) , Febriansyah, Rifki ( Universitas Muhammadiyah Yogyakarta )



Article Info

Publish Date
19 Apr 2014

Abstract

Eugenia uniflora Lam. is one of herbal products developed for anticancer. The aim of the present study was to identify the antiproliferative effect of ethanolic extract of Eugenia Uniflora Lam. leaves (EEU) on breast cancer cell line T47D. This Research was initiated by extracting the active contents of Eugenia uniflora Lam. leaves by maceration with ethanol 96%. The extract was then analyzed by thin layer chromatography (TLC). Cytotoxic assay of EEU was carried out by using MTT assay. Apoptosis phenomenon was observed with double staining using acridine orange-ethidium bromide. EEU showed cytotoxic effct on T47D cells with IC50 value of 65 μg/ml. Moreover, EEU 50 μg/ml and 100 μg/ml induced apoptosis. TLC examination showed that EEU used in this study contain phenolic, flavonoid, and saponin compounds which were suggested to be responsible for antiproliferative effect. Further molecular mechanism underlying EEU antiproliferative effect needs to be done.Keywords : Eugenia uniflora Lam., T47D cells, antiproliferative, apoptosis


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