Balaba
Vol 8, No 1 Jun (2012)

PEMERIKSAAN VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti YANG DIINFEKSI SECARA INTRATHORAKAL DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN ANTIBODI DSSE10

Widiastuti, Dyah (Unknown)
Umniyati, Sitti Rahmah (Unknown)
Wijayanti, Nastiti (Unknown)



Article Info

Publish Date
20 Sep 2013

Abstract

ABSTRACTDengue viruses, globally the most prevalent arboviruses, are transmitted to humans by persistently infectedAedes mosquitoes. The most important vector of Dengue virus is the mosquito Ae.aegypti, which should be the main targetof surveillance and control activities. Virologic surveillance for dengue viruses in its vector has been used as an earlywarning system to predict outbreaks. Detection of Dengue virus antigen in mosquito head squash usingimmunocytochemical streptavidin biotin peroxidase complex (SBPC) assay is an alternative method for dengue vectorsurveillance. The study aimed to develope immunocytochemical SBPC assay to detect Dengue virus infection in headsquash of Ae.aegypti. The study design was experimental. Artificially-infected adult Ae. aegypti mosquitoes of DENV 3were used as infectious samples and non-infected adult Ae. aegypti mosquitoes were used as normal ones. Theimmunocytochemical SBPC assay using monoclonal antibody DSSE10 then was applied in mosquito head squash todetect Dengue virus antigen. The results were analyzed by descriptive analysis. The immunocytochemical SBPC assaycan detect Dengue virus antigen in mosquito head squash at day 2 postinfection. There are some false positive resultsfound in immunocytochemical SBPC assay.Key Word: Dengue, immunocytochemistry, DSSE10

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Journal Info

Abbrev

blb

Publisher

Subject

Public Health

Description

Balaba (Berita dan Media Komunikasi Loka Litbang P2B2 Banjarnegara) terbit 2 kali setahun (Juni dan Desember). Merupakan majalah ilmiah populer didistribusikan secara gratis terbatas di lingkungan Kementerian Kesehatan dan lembaga penelitian / perguruan tinggi ternama di wilayah Provinsi Jawa ...