Jurnal AgroBiogen
Vol 14, No 2 (2018): December

Mapping of Resistance Genes to Brown Planthopper in Untup Rajab, an Indonesian Local Rice Variety

Yunus, Muhamad ( Balai Besar Penelitian dan Pengembangan dan , Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia )
Damayanti, Diani ( Balai Besar Penelitian dan Pengembangan dan , Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia )
Dadang, Ahmad ( Balai Besar Penelitian dan Pengembangan dan , Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia )
Warsun, Ahmad ( Balai Besar Penelitian dan Pengembangan dan , Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia )
Satyawan, Dani ( Balai Besar Penelitian dan Pengembangan dan , Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia )
Kusumanegara, Kusumawaty ( Balai Besar Penelitian dan Pengembangan dan , Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia )
Dewi, Iswari Saraswati ( Balai Besar Penelitian dan Pengembangan dan , Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia )
Sutrisno, Sutrisno ( Balai Besar Penelitian dan Pengembangan dan , Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia )
Husin, Bahagiawati Amir ( Balai Besar Penelitian dan Pengembangan dan , Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia )



Article Info

Publish Date
17 Dec 2018

Abstract

Brown planthopper (BPH) is a major rice pest in Indonesia. The most economical and effective approach to control the insect pest is by using resistant varieties. Exploring for resistance genes is, therefore, a prerequisite for effective breeding program for BPH resistance. This study aimed to map BPH resistance genes in Untup Rajab, an Indonesian local rice variety. Genetic map was constructed using an F2 population from a cross between TN-1 and Untup Rajab, and SNP markers from RiceLD SNP Chip. Phenotyping was performed using bulk seedling test on F2:3 seedlings against two BPH populations, i.e. X1 and S1. Four QTLswere identified on chromosomes 5, 6, 8, and 11 with PVE values of 7.63%, 9.40%, 17.66%, and 3.05%, respectively. Relatively normal distribution of resistance phenotype and the relatively low PVE values indicate that Untup Rajab has a quantitative resistance to BPH with two different resistance loci identified for each BPH test population. The QTL on chromosome 8 overlaps with OsHI-LOX gene, which is associated with resistance to BPH, and adjacent to another QTL for resistance to green leafhopper. The QTL on chromosome 6 was found near OsPLDĪ±4 and OsPLDĪ±5 genes which are related to BPH resistance. Meanwhile, the QTL intervals on chromosome 5 and 11 did not overlap with any known BPH QTLs or genes, which make them attractive candidates for novel BPH resistance gene discovery.

Copyrights © 2018






Journal Info

Abbrev

JAB

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya ...