HAYATI Journal of Biosciences
Vol 20, No 2 (2013): June 2013

Bacterial and Fungal Communities in Tempeh as Reveal by Amplified Ribosomal Intergenic Sequence Analysis

SEUMAHU, CECILIA ANNA ( Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University )
SUWANTO, ANTONIUS ( Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University )
RUSMANA, IMAN ( Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University )
SOLIHIN, DEDY DURYADI ( Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University )



Article Info

Publish Date
13 Jun 2013

Abstract

Tempeh is an Indonesian traditional fermented food produced using Rhizopus as a starter culture. In practice, however, the starter culture as well as fermentation processes would yield a polymicrobial fermentation, which generated a unique tempeh flavor and texture. This condition makes Indonesian tempeh as one of the most complex fermented food, while at the same time would make it difficult to scale up tempeh production with uniform quality and consistency. The aim of this study was to compare a number of tempeh microbial communities employing Amplified Ribosomal Intergenic Sequence Analysis (ARISA). Fresh tempeh samples were obtained from tempeh producers in Java and Moluccas. 16S rRNA gene libraries and DNA sequencing were employed to analyze further the nature of bacterial diversity in two selected tempeh samples. The results of our study showed that different tempeh producer possessed different Bacterial ARISA (BARISA) or fungi ARISA (FARISA) profiles.  However, BARISA profiles were found to be more discriminative than FARISA, and therefore BARISA would be more useful for tempeh genetic fingerprint or barcoding.

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Journal Info

Abbrev

hayati

Publisher

Subject

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

Description

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, ...