14 Sep 2013
The aim of the study was to investigate the role of zona pelucida (ZP) as a barrier of embryo againstE.coli K99 contamination. Â The complete randomized design was used in this study. Â The embryos weregiven treatment as a follow : 1) embryos without ZP were contaminated with E.coli K99; Â 2) embryo withintact ZP were contaminated with E.coli K99; Â and Â 3) embryos Â with intact ZP were not contaminated withE.coli K99, as a control. Â In each treatment there was 15 replication and in each replication there was oneembryo. Â The embryos were incubated in incubator at 37Â°C and 5% CO2 atmosphere. Â The embryos wereobserved every six hours in 24 hours using inverted microscope. Â The result showed that embryos withintact ZP could develop in culture contaminated with Â E.coli K99, while embryos without ZP becomedegenerated. Â The viability of intact embryos was 75% and the embryos without ZP were 65%. Â Embryosculture in contaminated medium could develop from eight cells embryo into morulla stage of embryo,compact morulla, and blastocyst. E.coli K99 contamination could inhibit embryo development. Â In conclusion,ZP could protect embryo against E.coli K99 contamination.
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