30 Jun 2015
The study aims to isolate and identify the natural antibacterial compounds potential from Aaptos suberitoidesÂ for Vibrio harveyi control on tiger shrimp (Penaeus monodon) culture. The agar diffusion method using paperÂ discs was used to determine the antibacterial activity of extracts (diethyl ether (DEE), butanol (BUE) andÂ aqueous (HOE) and compounds successfully isolated against Vibrio harveyi. Findings showed that theÂ antibacterial activity was concentrated in BUE with the inhibition zone of 17.2Â±0.1 mm. Meanwhile, twoÂ other extracts (DEE and HOE) did not exhibit any antibacterial activity against V. harveyi. From the activeÂ BUE, it was successfully isolated two compounds giving a strong anti-vibrio activity with the inhibitionÂ zone of 22Â±0.1 mm. The IR, 1H, 13C, COSY, HMQC, HMBC, and MS spectrum analysis indicated that bothÂ active compounds identified as aaptamine (1) and 9-demethyloxyaaptamine (2). The study suggested thatÂ marine sponge A. suberitoides may have potential compounds source for controlling of V. harveyi on tigerÂ shrimp culture.
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