The use of local probiotics in the culture of aquatic organisms is increasing with the demand for moreÂ environmental-friendly aquaculture practices. The local bacterium isolate considered as a probiotic wasÂ added into the water of whiteleg shrimp (Litopenaeus vannamei) culture in a field trial. Four rectangularÂ plastic ponds (ca. 20 m x 30 m per pond) were used for 100 days experimentation for six consecutive cropsÂ in two years experiment. Survival, harvest size, feed conversion ratio (FCR) and Vibrio bacterial count wasÂ compared with those of shrimp receiving and none of local isolate. Identification based on 16S rRNA geneÂ sequence shown those isolate was Bacillus pumilus strain DURCK14 with 99% homology. Water shrimp pondÂ added a local isolate had significantly higher survival at about 10.0% to 11.7% than shrimp without addedÂ the isolate (p<0.05), and better FCR, but no significant different in shrimp harvest size. Vibrio bacterial wasÂ undetected by total plate count. Moreover, it shown better projected yields on an annual basis (three cropsÂ per year).
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