Indonesian Aquaculture Journal
Vol 1, No 1 (2006): (June 2006)

GENETIC IDENTIFICATION FOR TUNA AND RAINBOW RUNNER CAPTURE IN NORTH BALI WATERS

Permana, Gusti Ngurah (Unknown)
Hutapea, Jhon Harianto (Unknown)
Haryanti, Haryanti (Unknown)
Sembiring, Sari Budi Moria (Unknown)
Nakazawa, Akio (Unknown)



Article Info

Publish Date
30 Jun 2006

Abstract

Gondol Research Institute for Mariculture identification of tuna and rainbow runner was an objective in this current study. Samples of five species were collected from territorial water of North Bali. The method used in this study was allozyme electrophoresis. The results showed that buffer of CAPM-6 (citric acid aminoprophylmorpholine) resulted in a sharp and clear banding pattern. The species could be differentiated in six diagnostic isozyme patterns Idh* (isocitrate dehydrogenase), 6Pgd* (6 phosphogluconate dehydrogenase), Gpi* (glucose phosphate isomerase), Mdh* (malate dehydrogenase), Est* (esterase), and Sp* (sarcoplasmic protein). All species were in Hardy-weinberg equilibrium. Heterozygosities of species were ranged from 0.00 to 0.099. Yellowfin tuna has the highest heterozigosity compared with the other species. Clustering samples according to pairs revealed that genetic distance of Bullet tuna (A. rochei) and Eastern little tuna (E. affinis) had small value (0.001). By contrast, the largest value was observed between yellowfin tuna, T. albacares and rainbow runner, E. bipunnulata (0.007). This value indicated that Bullet tuna (A. rochei) and Eastern little tuna (E. affinis) closed relation, while among yellowfin tuna, skipjack tuna, and rainbow runner, were separated phylogenically.

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Journal Info

Abbrev

iaj

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine ...