30 Dec 2007
Molecular biotechnology approach has been applied on sponge for preventingdiseases on fishery culture. This is important for anticipating and avoiding the usingÂ of amount of sponge in nature. The present study aims to screen the antimicrobialÂ (oxytetracycline and chloramphenicol) genes of sponge. DNA extraction of samplesÂ was done using the DNeasy Plant mini kit, Phenol-Chloroform and modification ofÂ Phenol-Chloroform methods. The presence of oxytetracycline and chloramphenicolÂ genes in sponge was detected using Polymerase chain reaction (PCR) technique.Â Result of the study showed that four species (Sylotella aurantium, Acanthella kletra,Â Gelliodes fibulatus and Auletta sp. were amplified for oxytetracycline and two speciesÂ (Auletta sp. and Pericharax sp.) of sponge were amplified for chloramphenicol atÂ each 226 bp.
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