Flame lily (Gloriosa superba L.)Â is one of the plants containing colchicine, often used as antimitotic compound, in the whole organ. This study aims to gain the combination of extract sourceÂ and extract concentration of G. superba capable of inhibit mitosis in root tip cells of chili Â sprouts.Â The plant isÂ extractedÂ Â using theÂ extraction and dilution methods to determine the extract concentration of the treatment. The squash method is used to make aÂ mitosis preparation. The study is arranged in a factorial using Randomized Complete Block Design (RCBD) with 4 replications. The first factor is extract source:Â Â roots, stems, and leaves. The second factor is the extract concentration: 0, 20.40, 60, and 80%.Â Â The observation shows that the extract of leaf can inhibit mitosis more than exctract of bulbs and roots.Â All of the concentration treatment from all extract sources tend to result in similar mitotic index, but significantly different from cells that are not given treatment. The combination of the source extract and concentration of the extract result inÂ varied mitotic index, butÂ it is obvious that the leaf extract with a concentration of 80% gives the most inhibiting mitosis index: 1.298%.
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