09 Oct 2012
Some reports have shown that ice cubes may contain Enterobacteriaceae bacteria. This descriptive, observational research aims to analyze the microbiological quality of the ice cubes served at three selected restaurants (1,2,3). Three samples of ice cubes were rinsed with steril water, left melted, and diluted serially. Each diluted sample was inoculated into MacConkey Agar and EMB Agar with pour plate method duplo. The data collected were the number of colonies (interpreted as cfu or colony forming unit) of Escherichia coli and other Enterobacteriaceae bacteria. The species of selected colonies were identified by biochemical reactions. The result showed that sample 1 contained 3 cfu/ml Escherichia coli and 5 cfu/ml Pseudomonas sp. Sample 2 contained 10 cfu/ml Escherichia coli and 4 cfu/ml Pseudomonas sp., but sample 3 didn’t contain Escherichia coli and other Enterobacteriaceae bacteria members. It is concluded that ice cubes from restaurant 3 met the requirement for drinking water, whereas those from restaurants 1 and 2 did not. Keywords: ice cubes, Escherichia coli, Pseudomonas sp.
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