Bacillus sp. BAC4 DNA was amplified using PCR method employing primers designed from pga genes of 5 bacteria (Arthrobacter viscosus, Bacillus megaterium, Escherichia coli, Kluyvera citrophila, and Providentia rettgeri). Analysis of DNA base direct sequencing of PCR products showed the highest homology of Bacillus sp. BAC4 DNA with Bacillus subtilis DNA at the region from citG to yirG. This fact states that primers designed from pga genes of 5 bacteria has not been successful in amplifying Bacillus sp. BAC4 pga gene, and that Bacillus sp. BAC4 pga gene is predicted to be quite different from the existing data of pga genes available at Genbank. Analysis of DNA base direct sequencing amplified using the pga primers shows the highest homology to the DNA of Bacillus subtilis.
Copyrights © 2008