BIOTROPIA
Vol 16, No 2 (2009)

ISOLATION AND CHARACTERIZATION OF METHANOTROPHIC BACTERIA FROM RICE FIELDS

Iman Rusmana (Unknown)
ALINA AKHADIYA (Unknown)



Article Info

Publish Date
14 Jul 2011

Abstract

Methane is a greenhouse gas and has potency to deplete ozone layer.  Rice fields are a significantly sources of atmospheric methane.  Chemical fertilizers application in rice fields can increase methane emission. Methanotrophic bacteria are unique in their ability to utilize methane as a sole carbon and energy source.  This research was successfully isolating and characterizing methanotrophic bacteria from rice fields in Bogor and Sukabumi, West Java, Indonesia.  Determination of methane oxidation activity using Gas Chromatography showed that all isolates performed methane oxidation activity.  The highest methane oxidation activity was performed by BGM 9 isolate. And DNA amplification of BGM 9 genome was performed single band of mmoX in the size of 500 bp and three bands of pmoA  in the size of 1000, 750 and 500 bp respectively

Copyrights © 2009






Journal Info

Abbrev

biotropia

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

BIOTROPIA, the Southeast Asian Journal of Tropical Biology is a scientific publication of SEAMEO BIOTROP. It publishes articles in the broad areas of tropical ecosystems and environmental impacts, biodiversity conservation and sustainable development, environmental and forest biotechnology, covering ...