Microbiology Indonesia
Vol 3, No 2 (2009): August 2009

The Relatedness between Hepatitis B Virus from Non-Papuan Blood Donors in Jayapura and the Papuan Clusters

NUGRAHAPUTRA, VICTOR EKA (Unknown)
AMIN, MOCHAMAD (Unknown)
MERTANIASIH, NI MADE (Unknown)
LUSIDA, MARIA INGE (Unknown)



Article Info

Publish Date
08 Aug 2010

Abstract

The genotypes (A-H) and subtypes (adw2, adw4, adrq-, adrq+, ayw1-4, ayr) of HBV show distinct geographical distributions, which have been associated with anthropological history. The novel finding of the HBV subgenotypes C6 and D6 from Papuans formed a specific cluster distinct from the previous HBV subgenotypes C1-C5 and D1-D5. In this study we determined the most recent genotype-subtype patterns of the HBV from non-Papuan blood donors who live in Jayapura and their phylogenetic relatedness, especially with the Papuan clusters. Fifteen HBsAg-positive serums were obtained from non-Papuan blood donors including from people in Java (46.7%), Maluku (26.7%), Sulawesi (20%) and East Nusa Tenggara (6.7%). S gene of all HBV serum isolates were partially sequenced and analyzed. Most HBV isolates (53.3%) were classified as genotype B, followed by genotype C(26.7%) and D (20.0%). The subtype adw2 (33.3%) was predominant, followed by adrq+ (26.7%) and ayw1/ayw2 (20.0%). All HBV isolates with subtype adw2 and ayw1 belonged to genotype B, while adrq+ belonged to genotype C and ayw2 belonged to genotype D. The most predominant HBV genotype-subtype (B/adw2) was consistent with the ethnic background (mostly from Java people). Nevertheless, based on the phylogenetic relatedness, many non Papuan isolates (40%) were classified into HBV/C6 and HBV/D6 of the Papuan clusters. Other isolates were classified into HBV/C1, HBV/B3 and HBV/B7. In conclusion, many HBV isolates from non-Papuans in Jayapura belonged to the Papuan clusters, but others had different genotype-subtype patterns with frequencies dependent on ethnicity.

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