Jurnal Akuakultur Rawa Indonesia
Vol 2, No 1 (2014): JURNAL AKUAKULTUR RAWA INDONESIA

TOKSISITAS DAN IMUNOGENISITAS PRODUK EKSTRASELULER Streptococcus agalactiae TIPE NON-HEMOLITIK PADA IKAN NILA (Oreochromis niloticus )

Dwinanti, Sefti Heza (Unknown)
., Sukenda (Unknown)
Yuhana, Munti (Unknown)
M Lusiastuti, Angela (Unknown)



Article Info

Publish Date
25 May 2015

Abstract

ABSTRACT The aim of this research was to analyse the toxicity and immunogenicity of ECP of non-hemolytic S. agalactiae and to evaluate ECP as a material vaccine for prevention of S. agalactiae. Toxicity to tilapia was detected in the ECP. There was a chronic  mortality pattern which was shown after post ECP injection. By intraperitoneal injection into young tilapia (± 20 g), the median lethal dose were calculated at 633,9 µg/kg bodyweight for isolate 3 and 685,4 µg/kg bodyweight for isolate 5. Cytopathic effects of ECP to tilapia’s lymphocytes were observed during 0, 30, 60 and 180 minutes. A mixture of ECP caused nuclear granulation and cytoplasmic streaming after 60 minutes. Using immunodiffusion analysis, precipitate line was demonstrated against tilapia anti-ECP sera, and there was bio-specific type anti sera against for each isolate. The ECP vaccine effective to protect tilapia from S. agalactiae was injected intraperitoneally (IP) at 2 x 105 CFU/ml. Tilapia vaccinated with ECP from isolat 3 which protein  283 µg/kg bodyweight had a RPS value 60%.  Tilapia vaccinated with ECP from isolat 5 which protein 408 µg/kg  had a RPS value 68%.   Keywords:  toxicity, immunogenicity, extracellular products, Streptococcus agalactiae, tilapia

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Journal Info

Abbrev

jari

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

Jurnal Akuakultur Rawa Indonesia merupakan jurnal ilmiah yang menerbitkan artikel hasil penelitian atau ulasan (literature review) tentang Budidaya Perairan (aquaculture). Jurnal Akuakultur Rawa Indonesia diterbitkan dua kali setahun yaitu pada bulan Juni dan ...