Indonesian Journal of Animal and Veterinary Sciences
Vol 7, No 2 (2002): JUNE 2002

The development of an Enzyme Linked Immunosorbent Assay for detecting Injectious laryngotrachitis viral antibodies in chicken serum

Indriani, Risa (Unknown)
Adjid, R.M Abdul (Unknown)
., Darminto (Unknown)
Hamid, Helmy (Unknown)



Article Info

Publish Date
17 Feb 2014

Abstract

The aim of this study was to develop an Enzyme-linked immunosorbent assay (ELISA) for detection of antibody against gallid herpes virus, the causal agent of infectious laryngotracheitis (ILT) in chicken. Its application in experimental chicken under laboratory condition was also evaluated. Results showed that ELISA for ILT was developed successfully with sensitivity and specificity was 98% and 97,14% respectively. The ELISA was able to determine the dynamic of antibodies respond in experimental chickens following vaccination and artificial infection with ILT virus. It was concluded that this ELISA offers a simple, sensitive and specific antibody assay for detection of antibodies against ILT virus in chickens arising from vaccination or infection.   Key words: ELISA, antibody, chicken, Infectious laryngotrachitis

Copyrights © 2002






Journal Info

Abbrev

JITV

Publisher

Subject

Veterinary

Description

JITV (Indonesian Journal of Animal and Veterinary Science),  ISSN: 0853-7380 E-ISSN: 2252-696X is a peer-reviewed, scientific journal published by Indonesian Center for Animal Research and Development (ICARD). The aim of this journal is to publish high-quality articles dedicated to all aspects ...