Indonesian Journal of Tropical and Infectious Disease
Vol 4, No 4 (2013)

MMUNOHISTOCHEMICAL ANALYSIS OF NF-kB (P0/P) IN PATIENT WITH AGGRESSIVE AND CHRONIC PERIODONTITIS

Prahasanti, Chiquita ( Department of Periodontia-Faculty of Dentistry, Universitas Airlangga Surabaya - Indonesia )



Article Info

Publish Date
06 Jul 2015

Abstract

Background: Nuclear factor-kappaB (NF-κB) is a protein complex that plays a role in transcription factors and in response to inflammation. Periodontitis is a periodontal disorder caused by various bacteria such as A. actinomycetemcomitan and P.gingivalis whose LPS is closely related to NFκB (p50/p65). Aim: This study observed whether NF-κB (p50/p65) played a role in aggressive and chronic periodontitis. Methods: Data were obtained from periodontal tissue 40 patients with aggressive periodontitis and 40 patients with chronic periodontitis. Samples were derived from periodontal tissue with abnormalities and NFκB (p50/p65) protein expression test was performed by immunohistochemistry. The statistical test used was the t-test. Results: In NF-κB (p50) the t value was -12 041 and significance 0.000, with α = 5%, showing significant difference in protein expression of NF-κB (p50) between patients with aggressive periodontitis and chronic periodontitis. OR estimation for the value of protein expression of NFκB (p50) was 0.64 (sign. = 0.000). It shows that if the protein expression of NFκB (p50) of the respondents is incremented by 1 (one) unit, the risk of chronic periodontitis increases 1.64 times. Box plot diagram shows that the distribution of the protein expression of NFκB (p50) between patients with aggressive periodontitis and chronic periodontitis patients is significantly different. In NF-κB (p65) the Z value was -7.137 and significance of 0000, with α= 5%, showed significant differences in protein expression of NF-κB (p65) between patients with aggressive periodontitis and chronic periodontitis. OR estimates for protein expression of NFκB (p65) was 0.66 (sign. = 0.000). This indicates that if the protein expression of NFκB (p65) respondents is incremented by 1 (one) unit, the risk of chronic periodontitis increases 1.5 times. Box plot diagram shows that the distribution of the protein expression of NFκB (p65) between patients with aggressive and chronic periodontitis patients is significantly different. Conclusion: The protein expression of NF-κB (p50/p65) has more influence on the incidence of chronic periodontitis patients, so it can be used as a marker for chronic periodontitis.

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Journal Info

Abbrev

IJTID

Publisher

Subject

Earth & Planetary Sciences Health Professions Medicine & Pharmacology Public Health

Description

This journal is a peer-reviewed journal established to promote the recognition of emerging and reemerging diseases specifically in Indonesia, South East Asia, other tropical countries and around the world, and to improve the understanding of factors involved in disease emergence, prevention, and ...