An acetone extract of Curcuma xanthorrhiza rhizomes and the n-hexaneÂ and chloroform fractions obtained from it were tested on eightÂ pathogenic bacteria. The results showed that the acetone extract and the nhexaneÂ fraction exhibited significant activities against Bacillus subtilis,Â Pseudomonas aeruginosa, and Staphylococcus aureus, and weak activitiesÂ against Shigella dysenteriae and Vibrio cholerae. They were inactive againstÂ Escherichia coli, Enterobacter aerogenes and Salmonella thypi, while theÂ chloroform fraction was devoid of activities. NMR analysis disclosed theÂ presence of Î±-curcumene, xanthorrhizol and an unknown monoterpene in the nhexaneÂ fraction. In the chloroform fraction, curcumin was found to be the mainÂ compound, together with xanthorrhizol as a minor compound. These resultsÂ suggest that the antibacterial potency of acetone extract of C. xanthorrhiza isÂ contained in the n-hexane fraction, in which the active constituents are terpenoidÂ compounds. This is the first report of the use of NMR analysis for compoundÂ identification contained in an extract or fractions of C. xanthorrhiza.
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