Indonesian Journal of Animal and Veterinary Sciences
Vol 1, No 3 (1995)

Evaluation of antibody responses of cattle and buffaloes to Clostridium perfringens type A vaccine using ELISA

Natalia, Lily (Unknown)

Article Info

Publish Date
25 Feb 2014


An enzyme-linked immunosorbent assay (ELISA) was developed to detect specific antitoxin Clostridium perfringens type A. It was based on the use of purified alpha toxin of Clostridium perfringens type A as the coating antigen, which was then linked to specific alpha antitoxin . Horse raddish peroxidase labelled IgG was used as the conjugate, and 2,2-azino-bis (3- ethylbenzthiazoline-6-sulfonic acid) (ABTS) as the substrate . The ELISA was used to evaluate vaccination results on cattle and buffaloes against enterotoxaemia caused by Clostridium perfringens type A. Key words: ELISA, Clostridium perfringens type A antitoxin, vaccination

Copyrights © 1995

Journal Info







JITV (Indonesian Journal of Animal and Veterinary Science),  ISSN: 0853-7380 E-ISSN: 2252-696X is a peer-reviewed, scientific journal published by Indonesian Center for Animal Research and Development (ICARD). The aim of this journal is to publish high-quality articles dedicated to all aspects ...