Indonesian Journal of Cancer Chemoprevention
Vol 1, No 2 (2010)

Apoptosis and Antioxidant Activities of Catharanthus rosues [L] G.Don Extract on Breast Cancer Cell Line

Widowati, Wahyu ( Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung )
Mozef, Tjandrawati ( The Indonesian Institute of Sciences, Bandung )
Risdian, Chandra ( The Indonesian Institute of Sciences, Bandung )
Ratnawati, Hana ( Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung )
Tjahyani, Susy ( Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung )
Sandra, Ferry ( Stem Cell and Cancer Institute, Jakarta )



Article Info

Publish Date
31 Jul 2012

Abstract

Tapak dara or Madagascar Periwinkle (Catharanthus roseus [L] G.Don), a natural plant, is empirically reported to have promising anticancer activity. To elucidate its mechanism, a research was conducted to investigate the possible ethanol extract of  C.  roseus in inducing apoptosis on breast cancer cell line (T47D). Antioxidant activity of C. roseus was investigated as well. Sub-G1 flowcytometric apoptotic analysis result showed that extract of C. roseus at 6.25 μg/mL induced apoptosis for 26.365%. Increasing extract concentration resulted an increasing apoptotic level as well, extract at concentration of 12.5 μg/mL induced apoptosis for 22.235%.  Meanwhile doxorubicin at concentration of 10  μg/mL induced apoptosis for 36.055%. The antioxidant activity was determined by using  in vitro assay: inhibition of  2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging  activity. Antioxidant activity of  C. roseus extract were compared to quercetin and butylated hydroxyanisol (BHA), as positive controls.  The results showed that DPPH IC50 of C. roseus extract, quercetin and BHA were 358.411 μg/mL, 19.200 μg/mL  and  94.178  μg/mL, respectively. We suggest that  C. roseus extract had a potential anticancer activity by inducing apoptosis.

Copyrights © 2010






Journal Info

Abbrev

ijcc

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

Description

Indonesian Journal of Cancer Chemoprevention (IJCC) is an open access, peer-reviewed, triannual journal devoted to publishing articles on Cancer Chemoprevention including Experimental and Clinical Pharmacology, especially concerning Anti-Oxidants, Anti-Aging, Anti-Inflammation, Anti-Angiogenesis, ...