HAYATI Journal of Biosciences
Vol 18, No 3 (2011): September 2011

Cloning and Expression Analysis of a Giant Gourami Vasa-Like cDNA

ALIMUDDIN, . ( Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University )
ANDRIYANI, IRMA ( Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University )
JUNIOR, MUHAMMAD ZAIRIN ( Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University )
ARFAH, HARTON ( Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University )
OCTAVERA, ANNA ( Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University )
CARMAN, ODANG ( Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University )
YOSHIZAKI, GORO ( Department of Marine Biosciences, Tokyo University of Marine Science and Technology )



Article Info

Publish Date
22 Dec 2011

Abstract

Molecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study, a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2,340 bps with an open reading frame of 1,962 bps encoding 653 amino acids. The deduced amino acid sequence contained 17 arginine-glycine or arginine-glycine-glycine motifs and eight conserved motifs belonging to the DEAD-box protein family. The GgVLG sequence showed high similarity to Drosophila vasa, common carp vasa homolog and tilapia vasa homolog for 66.2, 85.9, and 90.7%, respectively. In adult tissues, the GgVLG transcripts were specifically detected in ovary and testis. In situ hybridization analysis showed that GgVLG mRNA was detected in oocytes of the ovary and spermatogonia of the testis. There was no signal detected in the spermatocytes, spermatids and other gonadal somatic cells. Thus, consensus sequences, specific localization of GgVLG mRNA in the germ cells, amino acid sequence similarity and phylogenic analysis all suggest that GgVLG is the giant gourami vasa-like gene. Further, GgVLG can be used as a molecular marker for giant gourami germ cells.

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Journal Info

Abbrev

hayati

Publisher

Subject

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

Description

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, ...