HAYATI Journal of Biosciences
Vol 18, No 3 (2011): September 2011

Selective Media for In Vitro Activity Evaluation of Bacterial Biocontrol Against Pathogenic Vibrio

ISNANSETYO, ALIM ( Department of Fisheries, Faculty of Agriculture, Gadjah Mada University )
MUHTADI, . ( Department of Fisheries, Faculty of Agriculture, Gadjah Mada University )
ISTIQOMAH, INDAH ( Department of Fisheries, Faculty of Agriculture, Gadjah Mada University )
NITIMULYO, KAMISO HANDOYO ( Department of Fisheries, Faculty of Agriculture, Gadjah Mada University )
TRIYANTO, . ( Department of Fisheries, Faculty of Agriculture, Gadjah Mada University )



Article Info

Publish Date
22 Dec 2011

Abstract

In vitro activity test is a critical evaluation to screen the potential biocontrol agent. We developed a selective medium for quantitative in vitro activity evaluation of bacterial biocontrol agents against pathogenic Vibrio in aquaculture. Sensitivity test of bacterial biocontrol and Vibrio spp. to nine antibiotics showed that oxytetracycline inhibited the growth of Vibrio spp., but did not inhibit the growth of the bacterial biocontrol. This selective inhibition activity of oxytetracycline suggested this antibiotic might be supplemented to establish a selective medium. The MIC of oxytetracycline against V. harveyi, V. parahaemolyticus, and V. algosus were 120, 250, and 120 mg/ml, respectively. These concentration did not inhibit the growth of bacterial biocontrols. Therefore, oxytetracycline was supplemented at 250 µg/ml in Zobell agar medium. This medium was used as a selective medium to enumerate the density of bacterial biocontrol. In vitro activity test of bacterial biocontrol (RLP1) against V. parahaemolyticus showed that strain RLP1 at density of 104 and 106 cells/ml was able to kill V. parahaemolyticus during 6 h incubation. At lower density, 102 cells/ml, this bacterial biocontrol agent was able to kill the pathogenic Vibrio during 12 h incubation. This study discovered a selective medium for the bacterial biocontrol and Vibrio spp. and provided the results of its application in the evaluation of in vitro activity of a bacterial biocontrol agent against V. parahaemolyticus. The results also revealed that strain RLP1 is a potential bacterial biocontrol against vibriosis in marine aquaculture.

Copyrights © 2011






Journal Info

Abbrev

hayati

Publisher

Subject

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

Description

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, ...