Jurnal Veteriner
Vol 11, No 3 (2010)

Variasi Molekuler Gen Reseptor Melanokortin-4 pada Monyet Ekor Panjang


Arta Putra, I Gusti Agung, Sajuthi, Dondin, Duryadi Solihin, Dedy, Roro Dyah Perwitasari, Raden



Article Info

Publish Date
01 Sep 2010

Abstract

Melanocortin-4 receptor (MC4R) is one of G protein-coupled receptors that plays an important role inregulation of energy homeostasis. MC4R mutations constitute the most common cause of human obesity.The study was conducted in order to investigate the variation of MC4R gene in Macaca fascicularis and itsassociation with obesity as a model of human obesity. Forty eight adult male macaques from Bali (Ubudand Uluwatu), East Java (Alas Purwo and Baluran), and Sumatera island (Palembang) were used in thisresearch. The animals had been anaesthetized using ketamine (10 mg/kg body weight) and xylazine (2mg/kg body weight) before collecting blood samples and phenotypic data (weight, crown rump length. Bloodsamples were used as source of DNA. To determine MC4R variation, coding region of this gene wasamplified and sequenced. The results showed that 20 variations sites were identified and 13 of them werenon-synonymous. Among the non-synonymous mutations, five mutations were only found in obese macaque;two mutations were found both in obese and non-obese macaque; and six mutations were only found in nonobesemacaque .


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Original Source : http://ojs.unud.ac.id/index.php/jvet/article/view/3410
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Journal Info

Abbrev

jvet

Publisher

Subject

Veterinary

Description

Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah ...