Praja, Dedy Indra
Internal Medicine Department, Faculty of Medicine Universitas Indonesia-RSCM

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Pengaruh Kurkumin Terhadap Kadar NF-κB dan Derajat Fibrosis Hati pada Tikus Fibrosis Hati Supriono, Supriono; Pratomo, Bogi; Praja, Dedy Indra
Jurnal Penyakit Dalam Indonesia Vol 5, No 4 (2018)
Publisher : Internal Medicine Department, Faculty of Medicine Universitas Indonesia-RSCM

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Abstract

Pendahuluan. Inflamasi kronik merupakan mekanisme utama penyebab fibrosis hati. NF-κB  berfungsi mengatur inflamasi, penyembuhan luka, serta kematian sel. Kurkumin berperan sebagai antiinflamasi, antifibrotik, dan induksi apoptosis, salah satunya melalui hambatan terhadap NF-κB. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian kurkumin terhadap perubahan kadar NF-κB dan korelasi antara lama pemberian kurkumin terhadap kadar NF-κB dan derajat fibrosis hati.Metode. Penelitian ini merupakan penelitian eksperimental in vivo menggunakan 32 ekor tikus wistar yang dibagi dalam 8 kelompok perlakuan, tiap kelompok terdiri dari 4 ekor tikus. Perlakuan terhadap tikus berupa induksi CCl4 dan pemberian kurkumin. Pengukuran kadar NF-κB dengan metode ELISA. Derajat fibrosis hati menggunakan Metavir scoring system. Analisis data menggunakan uji korelasi, one-way Anova, unpair T-test, Mann-Whitney, Chi-square, Kruskal-Wallis dan analisis jalur model struktural dengan nilai p signifikan yaitu p<0,05. Hasil. Terdapat perbedaan yang signifikan kadar NF-κB dan derajat fibrosis hati antara kelompok kontrol positif dengan kelompok kontrol negatif. Terdapat pengaruh yang signifikan pemberian kurkumin dan lama pemberian kurkumin terhadap perubahan derajat fibrosis hati dan kadar NF-κB jaringan hati. Terdapat korelasi negatif antara lama pemberian kurkumin dengan perubahan kadar NF-κB dan korelasi positif antara perubahan kadar NF-κB dengan derajat fibrosis hati.Simpulan. Pemberian kurkumin dapat menurunkan kadar NF-κB dan derajat fibrosis hati. Lama pemberian kurkumin berkorelasi dengan penurunan kadar NF-κB dan penurunan kadar NF-κB berkorelasi dengan penurunan derajat fibrosis hati. Kata Kunci: Derajat fibrosis hati, Kurkumin, NF-κB, Tikus model fibrosis hati The Effects of Curcumin on NF-κB Level and Degree of Liver Fibrosis in Rat Liver Fibrosis Introduction. Chronic inflammation is the main mechanism responsible for liver fibrosis. NF-κB regulates inflammation, wound healing, and cell death. Curcumin acts as an antiinflammatory, antifibrotic and induced apoptosis, one of them through resistance to NF-κB. This study aimed to determine the effect of curcumin on NF-κB levels and the correlation between the duration of curcumin on NF-κB and degree of liver fibrosis. Methods. This study was an experimental in rats using a completely randomized design. The treatment of rats was induced with CCl4 and given curcumin. Measurement of NF-κB levels by ELISA method. Degree of liver fibrosis using metavir scoring system. The data were analyzed using correlation test, one-way anova, unpair T-test, mann-whitney, chi-square, kruskal-wallis, structural model with significant p value p < 0,05. Results. There were significant differences in NF-κB level and degree of liver fibrosis between the positive control group and the negative control group. There was a significant effect of curcumin administration on the level of NF-κB and degree of liver fibrosis. There was a negative correlation between duration of curcumin and NF-κB level and positive correlationbetween NF-κB levels and degree of liver fibrosis. Conclusions. Curcumin can decrease NF-κB levels and decrease the degree of liver fibrosis. The duration of the curcumin administration correlated with decreased levels of NF-κB and decreased NF-κB levels correlated with decreased degree of liver fibrosis in rat liver fibrosis. 
Association Between Patient Characteristics and Diet Profile with Kirsten rat sarcoma (KRAS) and Neuroblastoma rat sarcoma (NRAS) Gene Mutation in Colorectal Cancer Praja, Dedy Indra; Helena, Helena; Pratomo, Bogi
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy Vol 20, No 1 (2019): VOLUME 20, NUMBER 1, APRIL 2019
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

Background: Colorectal cancer is the third most common cancer according to American Cancer Society. It is also the third most common cause of death in men and women in US. Colorectal cancer encompasses 5% of all cancer and 29% of gastrointestinal cancer with men and women ratio is about 3:1. More than 1/3 of colorectal cancer occur below the age of 45 years. Mutation in Kirsten rat sarcoma (KRAS) gene was found in 30-50% of colorectal cancer in which it was suggested to associated with increase proliferation and decrease apoptosis. This study aimed to analyze the association between diet profile and KRAS gene mutation.Method: This study was a cross sectional study. Data was collected from medical records of colorectal cancer patient in Dr. Saiful Anwar General Hospital, which included KRAS gene mutation analysis.Results: There were 12 subjetcs included in this study. Four subjects (33.3%) had gene mutation with 3 subjects (75%) had positive KRAS mutation and 1 subjects (25%) had positive Neuroblastoma rat sarcoma (NRAS) mutation. In non-mutation group, it had been found a higher frequency of green leaf vegetables diet, in comparison with mutation group (p = 0.023). There was positive correlation between green leaf vegetables diet with gene mutation.Conclusion: Mutation of KRAS and NRAS mutation in colorectal carcinoma were found in 33.3% of subjects. Data analysis showed positive association between low green leaf vegetables diet with KRAS and NRAS mutation.
Association Between Patient Characteristics and Diet Profile with Kirsten rat sarcoma (KRAS) and Neuroblastoma rat sarcoma (NRAS) Gene Mutation in Colorectal Cancer Praja, Dedy Indra; Helena, Helena; Pratomo, Bogi
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy Vol 20, No 1 (2019): VOLUME 20, NUMBER 1, APRIL 2019
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Original Source | Check in Google Scholar | Full PDF (152.117 KB)

Abstract

Background: Colorectal cancer is the third most common cancer according to American Cancer Society. It is also the third most common cause of death in men and women in US. Colorectal cancer encompasses 5% of all cancer and 29% of gastrointestinal cancer with men and women ratio is about 3:1. More than 1/3 of colorectal cancer occur below the age of 45 years. Mutation in Kirsten rat sarcoma (KRAS) gene was found in 30-50% of colorectal cancer in which it was suggested to associated with increase proliferation and decrease apoptosis. This study aimed to analyze the association between diet profile and KRAS gene mutation.Method: This study was a cross sectional study. Data was collected from medical records of colorectal cancer patient in Dr. Saiful Anwar General Hospital, which included KRAS gene mutation analysis.Results: There were 12 subjetcs included in this study. Four subjects (33.3%) had gene mutation with 3 subjects (75%) had positive KRAS mutation and 1 subjects (25%) had positive Neuroblastoma rat sarcoma (NRAS) mutation. In non-mutation group, it had been found a higher frequency of green leaf vegetables diet, in comparison with mutation group (p = 0.023). There was positive correlation between green leaf vegetables diet with gene mutation.Conclusion: Mutation of KRAS and NRAS mutation in colorectal carcinoma were found in 33.3% of subjects. Data analysis showed positive association between low green leaf vegetables diet with KRAS and NRAS mutation.