Putriani, Putriani
Department of Biology, Faculty of Mathematics and Natural Sciences, University of Syiah Ku

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Isolasi, karakterisasi dan uji aktivitas antimikroba bakteri asam laktat dari fermentasi biji kakao (Theobroma cacao L.) Ismail, Yulia Sari; Yulvizar, Cut; Putriani, Putriani
Journal of BioLeuser Vol 1, No 2 (2017): Agustus 2017
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, University of Syiah Ku

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Abstract

Bakteri Asam Laktat (BAL) merupakan kelompok bakteri yang mampu mengubah karbohidrat  (glukosa) menjadi asam laktat. Penelitian ini bertujuan untuk mengisolasi dan mengetahui karakteristik BAL yang berperan dalam aktivitas fermentasi asam laktat pada biji kakao (Theobroma cacao L.).  Parameter yang diamati adalah morfologi koloni (bentuk, tepian, elevasi dan warna isolat), morfologi sel (bentuk sel dan pewarnaan Gram), uji biokimia (TSIA, indol, motilitas, MR-VP, tipe fermentasi, ketahanan suhu 15oC dan 37oC, ketahanan garam 5%, 6.5% dan 10%, serta katalase,) dan uji aktivitas antimikroba.  Dari hasil penelitian ini diperoleh tiga isolat BAL yang merupakan kelompok bakteri Gram positif berbentuk basil dan kokus. Ketiga isolat secara umum memiliki morfologi koloni yang berbeda yaitu berbentuk bundar, tepian licin, elevasi cembung dan datar, berwarna krem dan putih susu. Hasil uji biokimia menunjukkan bahwa ketiga isolat termasuk famili Lactobacillaceae, genus Lactobacillus (isolat FBK 1 dan FBK 3) dan genus Enterococcus (isolat FBK2).  Pada uji daya hambat (aktivitas antimikroba), ketiga isolat mampu menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus.
The Potential Endophytic Bacteria Isolated from Rice (Oryza sativa) as Biofertilizer Putriani, Putriani; Fitri, Lenni; Ismail, Yulia Sari
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.252 KB) | DOI: 10.15294/biosaintifika.v11i2.18401

Abstract

Endophytic bacteria are beneficial microorganisms that interact with host plants without causing any interference or damage to plants. This research aimed to obtain endophytic bacteria isolated from the root tissue of rice plants (Oryza sativa L.) which have potential to produce IAA hormones,, identify the endophytic bacteria in morphologically and physiologically, and analysis of the selected isolate 16S rRNA genes. Based on the results of this research, there  were six endophytic bacteria isolates obtained. They have high morphological diversity and differen ability producing IAA hormones. The highest concentration of IAA (425 ppm) was obtained  isolates from  EAP3. Isolate EAP3 also produce inhibit the growth of Xanthomonas oryzae with a 5.2 mm inhibition zone. Based on the biochemical test, EAP3 had 60% similarity with Enterobacter asburiae. Analysis of the 16S rRNA gene showed that EAP3 had the highest similarity with Enterobacter asburiae strain U4 by 99%. This research data is considered as new information about the potential of endophytic bacteria from the roots of rice plants (Oryza sativa L.) which is capable of producing IAA hormones and is able to inhibit pathogenic bacteria. This research provides information that can be used as a basis for developing endophytic bacteria as biological fertilizers. 
Pengaruh Tinggi Unggun Karbon Aktif Dan Pasir Kuarsa Pada Saringan Pasir Lambat Untuk Penyisihan Logam Fe Pada Air Sungai Siak Putriani, Putriani; Elystia, Shinta; Sasmita, Aryo
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Siak river water into the many natural resources provide benefits for the inhabitants of nearby, but the condition of the river siak currently included in the critical category. One of the treatments to reduce levels of pollutants in river water is by using a slow sand filter using quartz sand and activated carbon. This research aims to determined the effect of high activated carbon and quartz sand as well as the best sampling time with the efficiency of removal of Fe parameters in slow sand filters, and compared the quality standards of PP No. 82 of 2001. The fixed variable uses in this research is the velocity of water flow on the sand filter is slow 0.3 m / hour and the sampling time is 0, 15, 30, 45, 60. The changeable variable uses consists of variations in bed height in the reactor namely : 10 cm gravel, 50 cm quartz sand, and 20 cm activated carbon; 10 cm gravel, 60 cm quartz sand, and 10 cm activated carbon; and 10 cm gravel, 70 cm quartz sand, and no activated carbon. Based on the results of the research after the slow sand filtering process obtained the best removal efficiency in reactor 1 for 60 minutes, the efficiency of removal of Fe metal by 97.52%.Keywords: Siak River, Slow Sand Filter, activated carbon, quartz sand, Fe metal removal.