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NMR-metabolomics revealed metabolites and bioactivity variation in Torbangun leaves (Plectranthus amboinicus L.) of different origin Yuliana, Nancy Dewi; Sugiharto, Muhammad Anwari; Lioe, Hanifah Nuryanie; Goto, Masao; Ishikawa, Yuko Takano
Indonesian Journal of Biotechnology Vol 23, No 2 (2018): In Press
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.38659

Abstract

Plectranthus amboinicus (PA) were previously reported to have antidiabetic and antioxidant activities. Environmental factors might influence the plant’s  secondary metabolite profile and their health beneficial properties. NMR-metabolomics was used to show phytochemicals variation between the Plectranthus ambonicus grown in Japan and that grown in Indonesia. The results showed that flavonoids and triterpenes were among discriminating factors of the variation between the two groups. Targeted comparative analysis of the concentration of the specific flavonoids of the plants using a validated HPLC-MWD method showed that Japanese samples contained higher concentration of total flavonoids than that of Indonesian samples. The Japanese and Indonesian samples contained 1100.6 ± 5.1 and 532.4 ± 1.8 µg/g  luteolin , 584.5 ± 7.4 and 571.7 ± 11.6 µg/g  apigenin, respectively. Eriodyctiol was detected only in Indonesian samples. Contrarily, more intensive DPPH reduction and α-glucosidase inhibition activities were found in the  Indonesian samples (IC50 14.4 ± 1.2 and 24.0 ± 0.3 µg/mL for the DPPH assay, 1181.9 ± 113.5 and 4451.4 ± 290.0 µg/mL for α-glucosidase inhibition, respectively). Thus, the flavonoids might not the only group of compounds related to the bioactivity, but it should be confirmed  by further research targetting other groups of compounds such as triterpenes.