Hidayat, nFN
Masyarakat Iktiologi Indonesia (MII )

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KEKERAB ATAN BEBERAPA SPESIES IKAN PELANGI IRIAN (FAMILI MELANOTAENIIDAE) BERDASARKAN KARYOTIPE [The Closely Related of Some Rainbow Fishes (Melanotaeniidae) from Irian Based of Caryotipe] Said, Djamhuriyah S.; Hidayat, nFN
Jurnal Iktiologi Indonesia Vol 5, No 1 (2005): Juni 2005
Publisher : Masyarakat Iktiologi Indonesia (MII )

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Abstract

Family Melanotaenidae, known as Rainbow fishes consist of six genus and 53 species and distributed in Irian Jaya, Papua New Guinea, and Australia. Those fishes usually found in the river, swamp, or lakes which have clean water at about less than 1,500 in above sea level. The differentiation of the genus based on the shape of the jaw. Genus Melanotaenia has the most species (32 species). Some of them are endemic. The aim of the research is to explain the closely related of five species rainbow fishes: Glossolepis incisus/Gi, Melanotaenia boesemani/Mb, M. lacustris/Ml, M. maccullochi/Mm, and M. praecox/Mp based on the caryotype. The caryotype was examined on 2000. Diploid chromosome number of four species are 48 (2N=48) except M. lacustris lias (2N=46). Based on the Numeric Value of Chromosome Position(NVCP) the chromosome showed the shape were sub-metasentric, sub-telosentric, or telosentric. Analysis of the closely related of the five species studied based on NVC.P and relative chromosome length (RCL). Based on the NVCP showed Mp and Mm were in one group, than followed by Mb. Those three species (Mp, Mm, and Mb) made cne group with Gi. Melanotaenia lacustris has its own group. But based on the RCL analysis showed the different result. Analysis based on the shape of each number of chromosome and Gi as the outer (out group), Ml had the farthest relationship, so Ml has the farthest relationship with the four species.
STUDI KROMOSOM IKAN PELANGI (Melanotaenia lacustris) [Chromosome Study of Rainbowfish (Melanotaenia lacustris)] Said, Djamhuriyah S.; Carman, Odang; Hidayat, nFN; Abinawanto, nFN
Jurnal Iktiologi Indonesia Vol 3, No 2 (2003): Desember 2003
Publisher : Masyarakat Iktiologi Indonesia (MII )

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Abstract

Cytogenetic study of Melanotaenia lacustris was focussing on karyotype and to find out the information of chromosome. The research was conducted in Laboratory of Fish Breeding and Genetics, Faculty of Fisheries and Marine Science-IPB on May==December 2000. Chromosome plates were prepared by solid tissue technique and analyzed after staining with giemsa solution. Diploid chromosomes number of M. lacustris are (2n = 46). Karyotyping of this fish shown that 46 chromosomes consist of 9 pairs submetacentrik (SM) (no. 1, 3, 6, 7, 8, 9, 10, 13, and 14); 3 pairs subtelocentric (ST) (no. 4, 12, 22) and 10 pairs telocentric (T) (no. 2, 5, 11, 15, 16, 17, 18, 19, 20, and 21) with 1 ST and 1 T on the no. 23. Therefor is suggested that M. lacustris has a sex chromosome.AbstrakPenelitian sitogenetika pada ikan pelangi (Melanotaenia lacustris) difokuskan pada pengungkapan keanekaragaman kromosom dalam hal jumlah, bentuk, dan karyotipenya. Penelitian dilakukan di Laboratorium Genetika dan Reproduksi ikan Fakuitas Perikanan dan 11 mu Kelautan IPB, pada bulan on Mei — Deseber 2000. Preparat kromosom dibuat dengan metode jaringan padat yang menggunakan larva ikan umur 10-30 hari. Analisis kromosom dilakukan setelah pewarnaan dengan larutan Giemza. Kromosom diploid ikan M. lacustris adalah (2N = 46). Karyotipenya menunjukkan 46 kromosom yang terdiri atas 9 pasang berbentuk submetasentrik (SM) (no. 1, 3, 6, 7, 8, 9, 10, 13, and 14); 3 pasang subtelosentrik (ST) (no. 4, 12, 22) dan 10 pasang telosentrik (T) (no. 2, 5, 11, 15, 16, 17, IB, 19, 20, and 21) dengan 1 ST dan 1 T pada no. 23. Dari basil tersebut diduga bahwa M, lacustris memiliki kromosom sek.