Said, Djamhuriyah S.
Masyarakat Iktiologi Indonesia (MII )

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KEKERAB ATAN BEBERAPA SPESIES IKAN PELANGI IRIAN (FAMILI MELANOTAENIIDAE) BERDASARKAN KARYOTIPE [The Closely Related of Some Rainbow Fishes (Melanotaeniidae) from Irian Based of Caryotipe] Said, Djamhuriyah S.; Hidayat, nFN
Jurnal Iktiologi Indonesia Vol 5, No 1 (2005): Juni 2005
Publisher : Masyarakat Iktiologi Indonesia (MII )

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Abstract

Family Melanotaenidae, known as Rainbow fishes consist of six genus and 53 species and distributed in Irian Jaya, Papua New Guinea, and Australia. Those fishes usually found in the river, swamp, or lakes which have clean water at about less than 1,500 in above sea level. The differentiation of the genus based on the shape of the jaw. Genus Melanotaenia has the most species (32 species). Some of them are endemic. The aim of the research is to explain the closely related of five species rainbow fishes: Glossolepis incisus/Gi, Melanotaenia boesemani/Mb, M. lacustris/Ml, M. maccullochi/Mm, and M. praecox/Mp based on the caryotype. The caryotype was examined on 2000. Diploid chromosome number of four species are 48 (2N=48) except M. lacustris lias (2N=46). Based on the Numeric Value of Chromosome Position(NVCP) the chromosome showed the shape were sub-metasentric, sub-telosentric, or telosentric. Analysis of the closely related of the five species studied based on NVC.P and relative chromosome length (RCL). Based on the NVCP showed Mp and Mm were in one group, than followed by Mb. Those three species (Mp, Mm, and Mb) made cne group with Gi. Melanotaenia lacustris has its own group. But based on the RCL analysis showed the different result. Analysis based on the shape of each number of chromosome and Gi as the outer (out group), Ml had the farthest relationship, so Ml has the farthest relationship with the four species.
STUDI KROMOSOM IKAN PELANGI (Melanotaenia lacustris) [Chromosome Study of Rainbowfish (Melanotaenia lacustris)] Said, Djamhuriyah S.; Carman, Odang; Hidayat, nFN; Abinawanto, nFN
Jurnal Iktiologi Indonesia Vol 3, No 2 (2003): Desember 2003
Publisher : Masyarakat Iktiologi Indonesia (MII )

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Cytogenetic study of Melanotaenia lacustris was focussing on karyotype and to find out the information of chromosome. The research was conducted in Laboratory of Fish Breeding and Genetics, Faculty of Fisheries and Marine Science-IPB on May==December 2000. Chromosome plates were prepared by solid tissue technique and analyzed after staining with giemsa solution. Diploid chromosomes number of M. lacustris are (2n = 46). Karyotyping of this fish shown that 46 chromosomes consist of 9 pairs submetacentrik (SM) (no. 1, 3, 6, 7, 8, 9, 10, 13, and 14); 3 pairs subtelocentric (ST) (no. 4, 12, 22) and 10 pairs telocentric (T) (no. 2, 5, 11, 15, 16, 17, 18, 19, 20, and 21) with 1 ST and 1 T on the no. 23. Therefor is suggested that M. lacustris has a sex chromosome.AbstrakPenelitian sitogenetika pada ikan pelangi (Melanotaenia lacustris) difokuskan pada pengungkapan keanekaragaman kromosom dalam hal jumlah, bentuk, dan karyotipenya. Penelitian dilakukan di Laboratorium Genetika dan Reproduksi ikan Fakuitas Perikanan dan 11 mu Kelautan IPB, pada bulan on Mei — Deseber 2000. Preparat kromosom dibuat dengan metode jaringan padat yang menggunakan larva ikan umur 10-30 hari. Analisis kromosom dilakukan setelah pewarnaan dengan larutan Giemza. Kromosom diploid ikan M. lacustris adalah (2N = 46). Karyotipenya menunjukkan 46 kromosom yang terdiri atas 9 pasang berbentuk submetasentrik (SM) (no. 1, 3, 6, 7, 8, 9, 10, 13, and 14); 3 pasang subtelosentrik (ST) (no. 4, 12, 22) dan 10 pasang telosentrik (T) (no. 2, 5, 11, 15, 16, 17, IB, 19, 20, and 21) dengan 1 ST dan 1 T pada no. 23. Dari basil tersebut diduga bahwa M, lacustris memiliki kromosom sek.
PENGARUH PERLAKUAN JENIS PAKAN YANG BERBEDA TERHADAP PERTUMBUHAN IKAN PELANGI Marosatherina ladigesi [Effect of Feeds Type on Growth of Rainbowfish, Marosatherina ladigesi] Triyanto, nFN; Said, Djamhuriyah S.
Jurnal Iktiologi Indonesia Vol 6, No 2 (2006): Desember 2006
Publisher : Masyarakat Iktiologi Indonesia (MII )

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Abstract

The research about effect of feeds type on fish have common done. However information about appropriate feeds type for the growth of Marosatherina ladigesi has been unknown well. The research was conducted in Laboratory of Research Center for Limnology-LIPI in June - August 2006. The research aimed to determine the effect of feed to growth and survival rate of M. ladigesi. This Research conducted by utilizing four different feeds type, namely Daphnia, Chironomus, Tubificidae, and pellet. The result showed that different kind of feeds type have an effect on growth and survival rate of M. ladigesi. Feed from Tubificidae showed highest growth of total length 1.19 cm (0,020 cm/day). While feed type from Chironomus showed highest growth of weight 0.47 g (0.008 g/day) and highest survival rate 100%.
PENAMPILAN IKAN PANCHAX KUNING (Aplocheilus lineatus) PADA PEMBERIAN PAKAN YANG BERBEDA [The appearance of Aplocheilus lineatus on effect of different feed] Mayasari, Novi; Said, Djamhuriyah S.
Jurnal Iktiologi Indonesia Vol 8, No 2 (2008): Desember 2008
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Abstract

The research on effect of feeds type on fish have common done. However information about appropriate feeds type for the growth and performance of Aplocheilus lineatus has been unknown well. The aim of this study is to determine the effect of different feed to growth, survival rate (SR) and body colour of A. lineatus. Three different feeds was used in this study, were commercial diet (pellet), Tubificidae, and Chironomus. Each treatment repeated three times. Ten A. lineatus juveniles 1.2 - 2.1 cm in length were kept in 60 x 30 x 30 cm5 aquarium for 3 months with observation period every two weeks. The result shows that there was not significant survival rate of/1, lineatus (p > 0.05). There is significant different in daily growth (length and weight) and body colour. Fish which fed with Tubificidae show the best growth of length (O.OS36 cm/day) and the best growth of weight (0.0327 g/day). Difference of appearance of fish body which clearly seen in male fish. Colour of fish body relatively shines and more brightly in fish which fed with Chironomus.
KERAGAAN GENETIK IKAN SIDAT (Anguilla marmorata) DARI PERAIRAN POSO BERDASARKAN POLIMORFISME MITOKONDRIA DNA D-LOOP [Genetic Appearances of Eel (Anguilla marmorata) from Poso Waters Based on Mitochondria Polymorphism DNA D-loop] Triyanto,, nFN; Lukman, nFN; Said, Djamhuriyah S.
Jurnal Iktiologi Indonesia Vol 8, No 2 (2008): Desember 2008
Publisher : Masyarakat Iktiologi Indonesia (MII )

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Abstract

Anguilla marmorata is the dominant eel found in Poso. It has the largest body size and distributes on all habitat types especially the lake and the river of Poso. As catadromous fish, the larvae of eel grow in marine ecosystem which subsequently migrates and develops in freshwater ecosystem such as lakes and rivers. Information concerning variance of morphologies from the eel has been known but biological information especially in genetic not yet many reported. A. marmorata from various locations in Lake Poso (Pendolo, Tentena, Solokaya) and River Poso (Pandiri and estuary) was examined using polymorphism of the mitochondria DNA (mtDNA) D-loop markers. Four composite haplotypes were detected using digestion of D-loop sequences with five endonucleases: HaeIII, Hin61, /?sal, Taq\, and NdeII. There was no significant difference observed on the genetic variation among the eel populations.