Fadliyah, Hilyatul
Universitas Gadjah Mada

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Ekstrak n-Heksana Jinten Hitam (Nigella sativa Linn) Meningkatkan Aktivitas Estrogenik pada Sel CHO-K1 Fadliyah, Hilyatul; Dilalah, Idlohatud; M, Georgorius Gena; A, Faradiba Nur; S, Nadya Rizky; Jenie, Riris Istighfari
Jurnal Tumbuhan Obat Indonesia Vol 10, No 2 (2017): Jurnal Tumbuhan Obat Indonesia
Publisher : Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jtoi.v10i2.7184.63-69

Abstract

  Estrogen berperan dalam proliferasi sel, diferensiasi, dan fungsi organ spesifik. Defisiensi hormon estrogen menimbulkan berbagai gangguan fungsi fisiologis. Fenomena defisiensi estrogen dapat diatasi dengen memanfaatkan fitoestrogen. Penelitian ini bertujuan untuk mengetahui potensi jinten hitam dalam memodulasi efek estrogenik pada sel CHO-K1. Jinten hitam dimaserasi selama 42 jam untuk mendapatkan ekstrak kental dan kemudian digunakan dalam penelitian ini. Analisis fitokimia ekstrak kental diidentifikasi kandungan timokuinon menggunakan kromatografi lapis tipis (KLT). Uji aktivitas estrogenik meliputi uji sitotoksik dan proliferasi menggunakan metode MTT, uji pengamatan ekspresi protein penanda proliferasi menggunakan metode Western blot, dan uji interaksi molekuler senyawa timokuinon dengan protein proliferasi secara in silico. Bercak pada kromatogram menunjukkan posisi yang berdekatan dengan bercak pembanding yang digunakan. Hasil uji sitotoksik menunjukkan fenomena proliferasi diprediksi terjadi pada konsentrasi di bawah 200 µg/mL dan terkonfirmasi pada uji proliferasi. Intensitas ekspresi protein penanda proliferasi, cyc-e, paling tinggi terjadi pada konsentrasi 100 µg/mL. Data-data in vitro ini diperkuat dengan data in silico yang membuktikan bahwa kemungkinan interaksi antara senyawa kandungan EJH dengan senyawa target dapat terjadi karena lingkungan yang sama. Dengan demikian, dapat dikatakan EJH mampu memodulasi efek estrogenik pada sel CHO-K1 dengan kecenderungan meningkatkan efeknya. EJH potensial dikembangkan sebagai produk fitoestrogen selanjutnya.
Phaleria macrocarpa Fruit Extract Inhibits NF-ĸB Activation and Apoptosis Induction on HeLa Cells Zulharini S., Meirizky; Miranda, Amalia; Permatasari, Lina; Fadliyah, Hilyatul; Jenie, Riris Istighfari
Indonesian Journal of Cancer Chemoprevention Vol 8, No 1 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev8iss1pp9-14

Abstract

NF-κB is a transcription factor and if activated, it induces apoptosis inhibition. Phalerin from Phaleria macrocarpa fruits expected to inhibit NF-κB activation. This research is to investigate anticancer mechanism of Phaleria macrocarpa fruits extracts (EBMD) in NF-κB pathway. Molecular docking assay was performed to determine phalerin affinity to IKK. Cytotoxic activity was observed by MTT assay. Double staining was performed to determine the apoptotic cells. Docking score of phalerin to IKK is -60. The IC50 value of EBMD is 629 μg/mL. Apoptosis profile shows (shown that) many cells undergoing apoptosis after treatment. Thus, EBMD potentially inhibits activation of NF-κB pathway and triggers apoptosis on HeLa cells.Keywords : NF-κB, Phaleria macrocarpa, sel HeLa, Bcl-2, IKK, molecular docking
Anti-metastatic Profiles of Boesenbergia pandurata towards MCF-7/HER2 Cells Fadliyah, Hilyatul; Putri, Nindya Budiana; Walidah, Ziana; Nurhayati, Ika Putri; Ikawati, Muthi; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 9, No 2 (2018)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev9iss2pp68-77

Abstract

The development of breast cancer at an advanced stage is signed with metastatic phenomenon, triggering the high mortality, mainly for Human Epidermal Growth Factor Receptor (HER)2 positive cancers. Boesenbergia pandurata is well known as medicinal plant possessing anticancer potential due to the cytototoxic and antimetastatic characteristic of its active compound. The aim of this study is to observe the inhibitory effect of Boesenbergia pandurata ethanolic extract (BPEE) in combination with doxorubicin on migration of MCF-7/HER2 cells. The BPEE was prepared by 96% ethanol maceration. Under MTT assay, BPEE decreased the cells viability with IC50 value of 23±3.9 μg/mL. Lamellipodia and wound healing assay analysis showed that 5 μg/mL BPPE and its combination with 10 nM doxorubicin inhibited cells migration after 48 hours observation, while gelatin zymography analysis showed that this combination did not affect the expression of Matrix Metalloproteinase (MMP)2 and MMP9, but single treatment of 5 μg/mL BPEE caused lower expression of both MMPs. The combination of 5 μg/mL BPPE and 10 nM doxorubicin inhibited the cells migration but not affect to the cells viability. Thus, BPEE is potential to be developed as an antimetastatic agent. The mechanism underlying the migratory inhibition effect needs to be explored further.Keywords : Boesenbergia pandurata, doxorubicin, MCF-7/HER2, migrationSubmitted:
Fingerroot (Boesenbergia pandurata) : A Prospective Anticancer Therapy Nurrachma, Marsya Yonna; Fadliyah, Hilyatul; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 9, No 2 (2018)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev9iss2pp102-109

Abstract

Beside as a spice in Indonesian cooking, Fingerroot (Boesenbergia pandurata) regularly is used as a mixture of herbal medicine. Scientifically, the phytochemical content of the fingerroot rhizome showed some therapeutical effects such as antibacterial, anti-inflammatory, anti or pro-oxidant, and also anticancer. In this article, we summarize some studies especially about anticancer activity of fingerroot and its constituent coumpound. We found that fingerroot is capable of inhibiting various pathways of cell physiology processes. One potential pathway to be inhibited by fingerroot is Poly (ADP-ribose) polymerase (PARP) which has role in apoptotic induction. In the future, it is necessary to purify the extract to obtain maximum efficacy and also formulation studies of fingerroot will be interesting to do to.Keywords : fingerroot, anti-cancer, chemopreventive, herbal medicineBeside as a spice in Indonesian cooking, Fingerroot (Boesenbergia pandurata)regularly is used as a mixture of herbal medicine. Scientifically, the phytochemical contentof the fingerroot rhizome showed some therapeutical effects such as antibacterial, antiinflammatory,anti or pro-oxidant, and also anticancer. In this article, we summarizesome studies especially about anticancer activity of fingerroot and its constituentcoumpound. We found that fingerroot is capable of inhibiting various pathways of cellphysiology processes. One potential pathway to be inhibited by fingerroot is Poly (ADPribose)polymerase (PARP) which has role in apoptotic induction. In the future, it isnecessary to purify the extract to obtain maximum efficacy and also formulation studiesof fingerroot will be interesting to do to.
Red Betel Leaves Methanolic Extract (Piper crocatum Ruiz & Pav.) Increases Cytotoxic Effect of Doxorubicin on WiDr Colon Cancer Cells through Apoptosis Induction Wulandari, Nindi; Meiftasari, Argandita; Fadliyah, Hilyatul; Jenie, Riris Istighfari
Indonesian Journal of Cancer Chemoprevention Vol 9, No 1 (2018)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev9iss1pp1-8

Abstract

Doxorubicin is a chemotherapeutic agent that causes a lot of side effects in high doses. Thus, combination with a co-chemotherapeutic agent which can increase its toxicity on cancer cells, is needed to reduce its therapeutic dose. Red betel leaves (Piper crocatum Ruiz & Pav.) have been known to contain flavonoids and alkaloids that have anticancer activity. This study was conducted to determine the cytotoxic effect and apoptosis induction of red betel leaves methanolic extract (RBM), doxorubicin (dox) and the combination of them on WiDr cells as model of colon cancer. RBM was extracted by soxhlet method using methanol. Cytotoxicity assay was performed using MTT assay for both single and combination treatments for 24 hours to determine IC50 and CI as their parameters. Apoptosis induction was analyzed by double staining method using ethidium bromide-acridine orange staining. Treatment of RBM and dox on WiDr cells for 24 hours showed cytotoxic activity with IC50 100 μg/mL and 1.6 μM respectively. Combination of RBM and dox performed synergism effect with CI<0.9 (p<0.05). Combination of RBM (12.5 μg/mL) and dox (0.4 μM) increased the number of apoptosis cells compared to each single treatment. Based on this study, it can be concluded that red betel leaves methanolic extract is potential to be developed as a co-chemotherapeutic agent of doxorubicin on colon cancer but still need further study to disclose the underlying molecular mechanisms.Keywords :  Red betel leaves (Piper crocatum Ruiz & Pav.), doxorubicin, WiDr cells, co- chemotherapeutic agent
Antigenotoxic Activity of Rumput Mutiara (Hedyotis corymbosa L.) Ethanolic Extract on Cyclophosphamide-Induced Mice Aprianto, Yoce; Putri, Asri Mega; Fadliyah, Hilyatul; Murwanti, Retno; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 8, No 3 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev8iss3pp135-145

Abstract

Exposure to relative chemicals has been shown to induce a genotoxic effect that can be observed through formation of micronucleus (MN) in polychromatic erythrocythes (PCE). Rumput Mutiara or Hedyotis corymbosa L. ethanolic extract (HcEE) is known to contain ursolic acid as major compound that possesses antigenotoxic activity on HepG2 cells. This study exerts in vivo approach aiming to evaluate the antigenotoxic effects of HcEE on cyclophosphamide (CP)-induced male Swiss mice. The ursolic acid on HcEE was determined by using thin layer chromatography with silica gel as stationary phase and chloroform-aceton (9:1) as mobile phase. The antigenotoxic activity was carried out by in vivo micronucleus test. Twenty four adult mice were equally divided into seven groups. Group I: control (untreated); group II: Na-CMC 0.5%; group III: CP 50 mg/kg BW; group IV: CP+HcEE 250 mg/kg BW; group V: CP+HcEE 500 mg/kg BW; group VI: CP+HcEE 1000 mg/kg BW; group VII: HcEE 1000 mg/kg BW. HcEE were given for seven days, while CP was administered on the last two days. On the seventh day, the peripheral blood from all mice were collected, smeared, and then stained with Giemsa. The frequencies of MNPCEs and %PCEs were evaluated. Molecular docking was performed to know the interaction between ursolic acid and CYP3A4 by using PLANTS software. There was similar hRF spot between HcEE with ursolic acid standard reference indicated that the extract almost positively contain ursolic acid. HcEE reduced MNPCEs significantly compared to CP group (p<0.05) and combination of CP with HcEE showed reduction of %PCEs (p<0.05). Based on molecular docking analysis, ursolic acid gave lower docking score than CP against CYP3A4 (PDB ID: 2V0M) and similar binding site on amino acid residues Ala 448, Ile 369, Thr 309, and Val 313. All of these data suggest that HcEE perform protective effect against CP-induced genotoxicity.Keywords: Antigenotoxic, Hedyotis corymbosa L., cyclophosphamide, micronucleus, molecular docking
Methanolic Extract of Red Betel Leaves (Piper crocatum Ruiz & Pav) Perform Cytotoxic Effect and Antimigration Activity toward Metastatic Breast Cancer Zulharini, Meirizky; Sutejo, Ika Rahmawati; Fadliyah, Hilyatul; Jenie, Riris Istighfari
Indonesian Journal of Cancer Chemoprevention Vol 8, No 3 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev8iss3pp94-100

Abstract

Breast cancer is one type of cancer with a high mortality rate due to its metastatic property. Red betel leaves (Piper crocatum Ruiz and Pav) has been known as herbal medicine containing biophenolic, such as apigenin and luteolin derivatives which has cytotoxic activity toward cancer cells. This study is intended to explore the inhibitory effect of Piper crocatum leaves methanolic extract (PCM) on cell proliferation and migration by using 4T1 cells as model of metastatic breast cancer. By using MTT assay, PCM performed cytotoxic activity in a dose dependent manner with IC50 value of 120 μg/mL. Wound healing assay revealed that migration inhibitory activity of PCM on 4T1 cells at the concentration of 30 µg/mL. In conclusion, PCM perform cytotoxic effect and antimigration activity toward metastatic breast cancer cells.Keywords : breast cancer cells, Piper crocatum Ruiz & Pav, cytotoxic, cell mgration